期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 95, 期 2, 页码 373-377出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.16-0156
关键词
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资金
- National Institutes of Allergy and Infectious Diseases [5T32AI007151, 5 T32AI07001-36, R01AI089819]
- UNC Summer Undergraduate Research Gold Fellowship for Connor Belson
- GlaxoSmithKline
- Population Research Infrastructure Program through - Eunice Kennedy Shriver National Institute of Child Health and Human Development [R24 HD050924]
- National Science Foundation [BCS-1339949]
- PATH MVI
Estimates of malaria transmission intensity (MTI) typically rely upon microscopy or rapid diagnostic testing (RDT). However, these methods are less sensitive than nucleic acid amplification techniques and may underestimate parasite prevalence. We compared microscopy, RDT, and polymerase chain reaction (PCR) for the diagnosis of Plasmodium falciparum parasitemia as part of an MTI study of 800 children and adults conducted in Lilongwe, Malawi. PCR detected more cases of parasitemia than microscopy or RDT. Age less than 5 years predicted parasitemia detected by PCR alone (adjusted odds ratio = 1.61, 95% confidence interval = 1.09-2.38, Wald P = 0.02). In addition, we identified one P falciparum parasite with a false-negative RDT result due to a suspected deletion of the histidinerich protein 2 (hrp2) gene and used a novel, ultrasensitive PCR assay to detect low-level parasitemia missed by traditional PCR. Molecular methods should be considered for use in future transmission studies as a supplement to RDT or microscopy.
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