Article
Orthopedics
Michael Hadjiargyrou, Leonidas Salichos, Peter Kloen
Summary: In this study, the miRNA profiles of normal bone, fracture callus, and nonunion tissues were compared. It was found that certain miRNAs were upregulated or downregulated in normal bone and healing tissues. Target genes and relevant biological processes and signaling pathways were also identified. This study enhances our understanding of the role of miRNAs in fracture repair.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Nanoscience & Nanotechnology
Haizhu Kuang, Jing Ma, Xinyu Chi, Qichen Fu, Qianzhe Zhu, Weiling Cao, Peng Zhang, Xin Xie
Summary: The researchers reported a new therapeutic tool called Exo@miR-26a that can target multiple genes by inducing translation repression and target mRNA degradation. Exo@miR-26a significantly promotes bone regeneration and further facilitates healthy bone regeneration by repressing osteoclast differentiation-related genes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Christian Deininger, Andrea Wagner, Patrick Heimel, Elias Salzer, Xavier Monforte Vila, Nadja Weissenbacher, Johannes Grillari, Heinz Redl, Florian Wichlas, Thomas Freude, Herbert Tempfer, Andreas Herbert Teuschl-Woller, Andreas Traweger
Summary: In this study, silk scaffolds with axially aligned pores were generated and treated with simulated body fluid to create a calcium phosphate coating. In vivo experiments demonstrated that silk scaffolds loaded with a low dose of rhBMP-2 and coated with calcium phosphate significantly improved bone regeneration, providing effective bone repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dong Zhang, Weidong Xiao, Changjiang Liu, Zheng Wang, Yuhang Liu, Yifeng Yu, Chao Jian, Aixi Yu
Summary: Nonunion and delayed union are common complications of diabetes mellitus that pose a serious health threat. Exosomes derived from adipose stem cells (ASCs) have been found to promote bone fracture healing in diabetes mellitus. In this study, the effects of ASCs-exos on bone marrow mesenchymal stem cells (BMSCs) and bone repair in a rat model of nonunion were evaluated. The results showed that ASCs-exos enhanced BMSC osteogenic differentiation and improved fracture repair in the rat model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Yuntong Zhang, Yang Xie, Zichen Hao, Panyu Zhou, Panfeng Wang, Shuo Fang, Lu Li, Shuogui Xu, Yan Xia
Summary: Repairing large bone defects remains a major challenge for orthopedic surgeons, but this study demonstrates a promising strategy using umbilical MSC-derived exosomes in combination with hyaluronic acid hydrogel and nanohydroxyapatite/poly-epsilon-caprolactone scaffolds to enhance bone regeneration in rats. The exosomes were found to promote angiogenesis and potentially regulate it through the miR-21/NOTCH1/DLL4 signaling axis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Endocrinology & Metabolism
Hao Zhang, Yan Hu, Xiao Chen, Sicheng Wang, Liehu Cao, Shiwu Dong, Zhongmin Shi, Yanxi Chen, Liming Xiong, Yunfei Zhang, Dianying Zhang, Baoqing Yu, Wenming Chen, Qining Wang, Peijian Tong, Ximing Liu, Jianzheng Zhang, Qiang Zhou, Feng Niu, Weiguo Yang, Wencai Zhang, Yong Wang, Shijie Chen, Jinpeng Jia, Qiang Yang, Peng Zhang, Yong Zhang, Jun Miao, Kuo Sun, Tao Shen, Bin Yu, Lei Yang, Lei Zhang, Dongliang Wang, Guohui Liu, Yingze Zhang, Jiacan Su
Summary: Osteoporotic fractures, prevalent in the elderly, bring significant social burdens. These fractures are characterized by poor bone quality, weak repair capacity, instability, and high failure rate of internal fixation. Common osteoporotic bone defects, such as proximal humerus, distal radius, tibia plateau, calcaneus, and spine, require appropriate materials for repair. The consensus from the Osteoporosis Group of Chinese Orthopaedic Association aims to standardize clinical practice in bone repair of osteoporotic fractures.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Kai Chen, Tao Yu, Xin Wang
Summary: Research found that exosome-derived miRNA from diabetic patients slows down wound healing process by suppressing vascular endothelial growth factor A expression.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Pharmacology & Pharmacy
Yang Zhao, Yujia Gong, Xianbo Liu, Jia He, Bowen Zheng, Yi Liu
Summary: This study investigated the osteogenic ability of periodontal ligament stem cell-derived exosomes (PDLSCs-Exos) and their effect on alveolar bone defect repairment. Results showed that PDLSCs-Exos significantly promoted the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. In vivo experiments revealed that the combination of PDLSCs-Exos and hydrogel led to more new bone formation in alveolar bone defects compared to the control group and the hydrogel group.
Review
Endocrinology & Metabolism
Miao Tian, Ying-bo Han, Gui-yun Yang, Jin-long Li, Chang-sai Shi, Dan Tian
Summary: Lactoferrin (Lf) is a multifunctional protein that plays critical roles in bone remodeling by regulating the function of various cell types involved in the process. It stimulates osteoblast and chondrocyte activity while inhibiting osteoclast function, leading to accelerated fracture repair and improved bone health. These effects have driven the exploration of Lf as a potential therapeutic target for bone-related diseases. However, delivery strategies need to be developed to enhance its bioavailability at the target site. Overall, the importance of Lf in bone remodeling and its potential therapeutic applications warrant a score of 8 out of 10.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Medicine, Research & Experimental
Rui Liu, Yuping Zhong, Rui Chen, Chengchao Chu, Gang Liu, Yong Zhou, Yazhu Huang, Zhihong Fang, Huan Liu
Summary: This study demonstrates that myeloma cells hnRNPA2B1 mediates microRNAs processing, leading to activation of osteoclastogenesis and suppression of osteoblastogenesis. Clinical studies show a positive correlation between myeloma cells hnRNPA2B1 level and osteolytic bone lesions.
Review
Orthopedics
Ahmed Khalil Attia, Greg A. J. Robertson, John McKinley, Pieter P. d'Hooghe, Nicola Maffulli
Summary: This study aimed to determine the effect of orthobiologic augmentation on the outcome of surgically managed Jones fractures in athletes. The findings suggest that biologically augmented fixation can increase the fracture union rate, but has similar rates of return to play and time to return to play compared to fixation alone.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2023)
Review
Pharmacology & Pharmacy
Zhichao Li, Dandan Li, Hui Su, Haipeng Xue, Guoqing Tan, Zhanwang Xu
Summary: The article discusses the importance of the balance between bone resorption and formation for bone homeostasis, and the role of autophagy in this process. It also highlights the potential of natural products in mediating autophagy for the treatment of metabolic bone diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Engineering, Multidisciplinary
Junping Deng, Qun Song, Siyuan Liu, Wenhui Pei, Peng Wang, Liming Zheng, Caoxing Huang, Mingguo Ma, Qing Jiang, Kai Zhang
Summary: Cellulose and its derivatives are bio-composites widely used in bone and cartilage tissue engineering, but there are still shortcomings that need to be addressed for their better application in bone disease treatments.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Medicine, General & Internal
Sebastian Findeisen, Melanie Schwilk, Patrick Haubruck, Thomas Ferbert, Lars Helbig, Matthias Miska, Gerhard Schmidmaier, Michael Christopher Tanner
Summary: This study aimed to determine the influence of defect size on the outcome of atrophic and infected nonunions of the tibia or the femur. It was found that defect size was significantly correlated with the time required for bone consolidation, but had no significant effect on the consolidation rate. Therefore, for the treatment of large-sized bone defects, a prolonged follow-up period and careful consideration of early revision are necessary.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Orthopedics
Katherine R. Hixon, Anna N. Miller
Summary: Bone healing after injury is a systematic and spontaneous process, but impaired healing can still occur and may require surgical intervention. Different forms of impaired bone healing include nonunion, critical-size defects, and stress fractures. This review focuses on animal models used to study these defects in vivo and discusses current treatment methods and potential future therapies.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Surgery
Vamiq M. Mustahsan, Guangyu He, Carlos G. Helguero, Christopher L. Blum, David E. Komatsu, Srinivas Pentyala, Imin Kao, Fazel Khan
Summary: The use of an inexpensive optical feedback system (OFS) can accurately depict the placement of surgical guides and significantly improve the accuracy of 3D printed jigs.
SURGICAL INNOVATION
(2023)
Review
Endocrinology & Metabolism
Michael Hadjiargyrou
Summary: The balance between osteoclastic bone resorption and osteoblastic bone formation is crucial for maintaining a healthy skeleton. Osteoporosis, characterized by increased bone resorption and decreased bone formation, leads to weaker bones and a higher risk of fractures. Antiresorptive drugs are commonly used for treatment, but their long-term use may increase the risk of atypical femoral fractures.
Article
Biology
Edwin Dickinson, Melody W. Young, Charles J. Kim, Michael Hadjiargyrou, Michael C. Granatosky
Summary: The ability to securely grasp substrates of variable diameter is critical for arboreal animals. This study examines the pulling and gripping performance of a model parrot species across a range of substrate diameters. The results show that parrots have optimal grip force on small perches, consistent with their preferential perching habits in the wild. Relative to other arboreal specialists, the grasping performance of parrots is comparable, but lower than that of raptorial birds. Further research is needed to understand the adaptive evolutionary context of grasping performance.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Alexander A. Chirokikh, Sardar M. Z. Uddin, Nadine Areikat, Rachel Jones, Edie Duque, Carly Connor, Michael Hadjiargyrou, Panayotis K. Thanos, David E. Komatsu
Summary: Methylphenidate (MP) is commonly used to treat Attention-Deficit/Hyperactivity Disorder (ADHD), but many patients with ADHD also experience depression and anxiety. Therefore, concurrent administration of selective serotonin reuptake inhibitors such as fluoxetine (FLX) is common. This study aimed to investigate the effects of MP and FLX on bone morphology and biomechanical properties in adolescent rats.
Article
Pharmacology & Pharmacy
Shebin Tharakan, Shams Khondkar, Sally Lee, Serin Ahn, Chris Mathew, Andrei Gresita, Michael Hadjiargyrou, Azhar Ilyas
Summary: In this study, alginate/collagen hydrogels loaded with MC3T3-E1 osteoblasts were 3D printed with Sr-Ca polyphosphate. The results showed that a concentration of 2 mg/mL of Sr-Ca polyphosphate induced the greatest degradation of scaffolds after 7 days and the least swelling after 24 h, while exposure of osteoblasts to 1 mg/mL of Sr-Ca polyphosphate caused severe cytotoxic effects. Overall, low doses of Sr-Ca polyphosphate showed potential as an additive in biomaterials for bone tissue engineering, but the cytotoxic effects due to its pH must be closely monitored.
Article
Biology
Carlyn Annunziata, Haniyeh Fattahpour, Daniel Fong, Michael Hadjiargyrou, Pejman Sanaei
Summary: In vitro tissue engineering scaffolds consist of pores that allow cells to migrate and are supplied with nutrient-rich culture medium. Cell proliferation in elastic scaffolds depends on the deformation of the scaffold pores, which is influenced by the fluid dynamics and nutrient flow rate. This mathematical model and simulation offer insights into the cellular growth within elastic scaffolds.
BULLETIN OF MATHEMATICAL BIOLOGY
(2023)
Review
Geriatrics & Gerontology
Bridget Boston, Deepak Ipe, Bogdan Capitanescu, Andrei Gresita, Stephen Hamlet, Robert Love, Michael Hadjiargyrou, Chien-Ling Huang, Iulian Nusem, Rodica Ileana Miroiu, Aurel Popa-Wagner, Patrick Hans-Heinrich Warnke, Eugen Bogdan Petcu
Summary: Medication-related osteonecrosis of the jaw (MRONJ) refers to the non-healing jawbone ulcerative-necrotic lesion appearing after dental therapy or minor trauma in patients treated previously with certain medications. Studies have been conducted to explore MRONJ's classification, clinical features, and pathophysiology, as well as its management and new trends in treatment. Conservative therapy may not be effective for severe cases of MRONJ, but new methods that promote local angiogenesis and vascularization show promise in limited trials.
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY
(2023)
Article
Orthopedics
Michael Hadjiargyrou, Leonidas Salichos, Peter Kloen
Summary: In this study, the miRNA profiles of normal bone, fracture callus, and nonunion tissues were compared. It was found that certain miRNAs were upregulated or downregulated in normal bone and healing tissues. Target genes and relevant biological processes and signaling pathways were also identified. This study enhances our understanding of the role of miRNAs in fracture repair.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Review
Health Care Sciences & Services
Daniela Senior, Rania Ahmed, Eliz Arnavut, Alexandra Carvalho, Wen Xuan Lee, Kenneth Blum, David E. Komatsu, Michael Hadjiargyrou, Rajendra D. Badgaiyan, Panayotis K. Thanos
Summary: The majority of animal studies on methylphenidate (MP) use methods such as intraperitoneal injections, subcutaneous injections, or the oral gavage route. However, the most clinically relevant route is oral administration. Intraperitoneal injections provide an immediate and maximum dose, but only represent a limited window of the drug's effects. Subcutaneous injections do not accurately reflect oral exposure, and the oral-gavage method has adverse effects and may be stressful for animals. The use of a two-bottle drinking method allows for free consumption of MP and more accurately mirrors human treatment, enabling the study of MP's effects on development, behavior, neurochemistry, and brain function.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Polymer Science
Shebin Tharakan, Iman Raja, Annette Pietraru, Elina Sarecha, Andrei Gresita, Eugen Petcu, Azhar Ilyas, Michael Hadjiargyrou
Summary: Osteosarcoma, a malignant bone tumor, poses significant risks to patient mortality and morbidity. Conventional invasive treatments have drawbacks, prompting researchers to explore the use of hydrogels for targeted therapy. Hydrogels loaded with chemotherapeutic drugs have demonstrated promising results in eradicating tumor cells and promoting bone regeneration. Stimuli-responsive hydrogels can react to the tissue microenvironment, facilitating controlled drug release with modulated biomechanical properties. This narrative review discusses in vitro and in vivo studies on different hydrogels, including stimuli-responsive ones, for the treatment of osteosarcoma, as well as potential future applications for patient treatment.
Article
Physiology
Charles J. Kim, Chanpreet Singh, Christine Lee, Kevin DiMagno, Madison O'Donnell, Marina Kaczmarek, Arhum Ahmed, Jessica Salvo-Schaich, Alexis Perez, William Letsou, Maria-Alicia Carrillo Sepulveda, Raddy L. Ramos, Michael Hadjiargyrou
Summary: Research indicates that Mustn1 is involved in skeletal muscle development and is associated with myogenic differentiation and myofusion. The role of Mustn1 in glucose homeostasis is unclear. By deleting Mustn1 in skeletal muscle, it was found that male mice had lower glycemia and altered gene expression related to glucose metabolism and insulin sensitivity at 2 months of age. However, these differences became insignificant after 4 months. There were no differences observed in female mice. These findings suggest a link between Mustn1 expression and glucose homeostasis during skeletal muscle development.
PHYSIOLOGICAL REPORTS
(2023)
Article
Chemistry, Physical
Andrei Gresita, Iman Raja, Eugen Petcu, Michael Hadjiargyrou
Summary: Successfully reconstructing bone and restoring its dynamic function is a significant challenge. Traditional bone grafting methods carry risks and alternatives are needed. In this study, collagen-coated hyperelastic bone scaffolds were found to support cell adhesion and proliferation, but exhibited reduced structural support after 7 days in culture.
Article
Medicine, Research & Experimental
Sandhya Ganesan, Ryan P. Tantone, David E. Komatsu, Lawrence C. Hurst
Summary: This retrospective cohort study analyzed the prevalence of Dupuytren's disease (DD) in diabetic patients using data from the TriNetX Research Database. The study found that patients with type 2 diabetes had a higher prevalence of DD compared to patients with type 1 diabetes. Among diabetic patients, those taking insulin had a higher prevalence of DD compared to those taking metformin. The prevalence of DD also varied based on HbA1c levels.
COMMUNICATIONS MEDICINE
(2023)