Article
Immunology
Lauren E. Higdon, Claire E. Gustafson, Xuhuai Ji, Malaya K. Sahoo, Benjamin A. Pinsky, Kenneth B. Margulies, Holden T. Maecker, Jorg Goronzy, Jonathan S. Maltzman
Summary: Immune function changes with age, and infection with CMV accelerates these changes, resulting in increased mortality and impaired responses in elderly individuals. Following organ transplantation, CMV+ recipients show accelerated aging of CD8 T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Surgery
Arne Sattler, Lion G. Thiel, Annkathrin H. Ruhm, Yasmin Bergmann, Theresa Dornieden, Mira Choi, Fabian Halleck, Frank Friedersdorff, Dennis Eurich, Katja Kotsch
Summary: Immunosuppressants can affect the activation and functional competence of MAIT cells, particularly in liver transplant recipients, leading to impaired activation and function both in vitro and in vivo.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Immunology
Ulrich Blache, Ronald Weiss, Andreas Boldt, Michael Kapinsky, Andre-Rene Blaudszun, Andrea Quaiser, Annabelle Pohl, Tewfik Miloud, Megane Burgaud, Vladan Vucinic, Uwe Platzbecker, Ulrich Sack, Stephan Fricke, Ulrike Koehl
Summary: The adoptive immunotherapy using CAR-T cells has shown successful remissions in refractory B-cell leukemia and B-cell lymphomas, but it is important to monitor both success and potential severe side effects of this therapy. Research has been conducted to establish standardized flow cytometry assays to characterize immune cell subpopulations in the peripheral blood during CAR-T cell treatment, with the possibility of adapting these assays for quality controls in the CAR-T cell manufacturing process in the future.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Surgery
Timna Agur, Johannes Wedel, Sayantan Bose, A. G. Pramoda Sahankumari, Daniel Goodman, Sek Won Kong, Chandra C. Ghosh, David M. Briscoe
Summary: The study demonstrates the impact of mevalonate on the immunoregulatory phenotype of endothelial cells and the inhibitory effect of simvastatin on mevalonate metabolism, leading to decreased T cell proliferation and pro-inflammatory cytokine production. These findings suggest the critical role of mevalonate metabolism in sustaining endothelial cell-dependent immune mechanisms.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Robin Reschke, Philipp Gussek, Andreas Boldt, Ulrich Sack, Ulrike Koehl, Florian Lordick, Thomas Gora, Markus Kreuz, Kristin Reiche, Jan-Christoph Simon, Mirjana Ziemer, Manfred Kunz
Summary: In melanoma patients, immune monitoring of PBMC for immune-checkpoint inhibition (ICI) treatment appears to be a promising approach to identify early markers of treatment response and immune-related adverse events (irAEs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
A. K. M. Arifuzzman, Norh Asmare, Tevhide Ozkaya-Ahmadov, Ozgun Civelekoglu, Ningquan Wang, A. Fatih Sarioglu
Summary: Characterization and identification of cell populations using surface markers are crucial in various applications. However, conventional techniques are limited by the need for immunolabeling, expensive instruments, and skilled operators. This study introduces an autonomous microchip that can electronically quantify the immunophenotypical composition of a cell suspension. The microchip captures different cell subtypes in separate microfluidic chambers and electrically monitors all on-chip activity, allowing real-time determination of subpopulation fractions. The capability to analyze immune cells without labels has the potential to enable remote testing and quality control in cell manufacturing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Surgery
Qiang Fu, Kang Mi Lee, Guoli Huai, Kevin Deng, Divyansh Agarwal, Charles G. Rickert, Noel Feeney, Rudy Matheson, Hongji Yang, Christian LeGuern, Shaoping Deng, James F. Markmann
Summary: Regulatory B cells (Bregs) have been found to have potential as anti-rejection therapy in organ transplantation, particularly Bregs-TLR cells stimulated by TLR ligands that can prevent allograft rejection. These Bregs-TLR cells can suppress B cell proliferation, leading to a decline in antibody responses to antigenically disparate skin grafts. Additionally, the specificity of Breg BCR plays a crucial role in regulation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Florian Mair, Jami R. Erickson, Marie Frutoso, Andrew J. Konecny, Evan Greene, Valentin Voillet, Nicholas J. Maurice, Anthony Rongvaux, Douglas Dixon, Brittany Barber, Raphael Gottardo, Martin Prlic
Summary: Immunotherapies have shown great success in cancer treatment, but face challenges. Current treatment approaches target pathways not only in tumors but also in other tissues, complicating treatment. Single-cell analysis revealed significant overlap in immune cell composition and phenotype between tumors and inflamed tissues.
Article
Medical Laboratory Technology
Hyunji Kim, Sung Shin, Sang-Hyun Hwang, Miyoung Kim, Young-uk Cho, Seongsoo Jang
Summary: The adoption of high-sensitivity flow cytometry (HSFC) has been slow in routine laboratory settings due to concerns about result reliability and reproducibility. This study aimed to validate an HSFC protocol for detecting Tfh cells in a real-world laboratory environment. The Tfh cell panel was analytically validated using rigorous testing, and concerns about result reliability and reproducibility in real-world laboratories were addressed through systematic validation. Strategic validation of flow cytometry panels can facilitate the adoption of HSFC in clinical laboratories, even with limited resources.
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Surgery
Aurore Prunevieille, Mohamed H. Babiker-Mohamed, Colleen Aslami, Bruno Gonzalez-Nolasco, Nuala Mooney, Gilles Benichou
Summary: Studies have shown that allogeneic exosomes can activate T cells in vivo and sensitize mice to alloantigens, but only when delivered in an inflammatory environment.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Pharmacology & Pharmacy
Chelsea B. Pratt, Matt Alexander
Summary: The development of CAR-T cells is crucial for the success of adoptive T-cell immunotherapy. While the process of producing autologous CAR-T cells is time-consuming and expensive, the exploration of cost-effective allogeneic CAR-T cells is essential. High-throughput tools are used to monitor the entire process of CAR-T cell design, testing, manufacturing, and patient monitoring to ensure safety and efficacy.
DRUG DISCOVERY TODAY
(2022)
Review
Medicine, General & Internal
Katya Prakash, Aditya Chandorkar, Kapil K. Saharia
Summary: CMV-specific cell mediated immunity (CMI) testing may be more effective in predicting CMV infection risk in solid organ transplant recipients compared to the current standard of care of anti-CMV IgG. Larger prospective trials are needed to further evaluate the effectiveness of these novel assays in high-risk populations.
Article
Surgery
Glenn Michael La Muraglia, Susan Zeng, Emma S. Crichton, Maylene E. Wagener, Mandy L. Ford, Idelberto Raul Badell
Summary: Research suggests that selective CD28 blockade compared to CTLA-4-Ig can better inhibit Tfh cells, germinal centers, and DSA responses, as well as more effectively control B cell responses. CTLA-4 expression is necessary and sufficient for the improved humoral inhibition observed with selective CD28 blockade.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Cell Biology
Martijn Cordes, Karin Pike-Overzet, Erik B. B. Van Den Akker, Frank J. T. Staal, Kirsten Cante-Barrett
Summary: Flow cytometry has traditionally been used to characterize immune cells and their markers. Single-cell RNA-sequencing has revolutionized the study of immune development, allowing for the analysis of heterogenous tissues and the integration of omics data at the single-cell level. This integration contributes to our understanding of the immune system by revealing the heterogeneity within cell populations identified by surface markers and the correlation between RNA and protein data.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Dimitrios N. Sidiropoulos, Genevieve L. Stein-O'Brien, Ludmila Danilova, Nicole E. Gross, Soren Charmsaz, Stephanie Xavier, James Leatherman, Hao Wang, Mark Yarchoan, Elizabeth M. Jaffee, Elana J. Fertig, Won Jin Ho
Summary: Mass cytometry, or CyTOF, can be used to determine protein-level measurements of more than 40 markers simultaneously. In this study, single-cell trajectory inference and nonnegative matrix factorization methods were applied to CyTOF data to track the dynamics of T cell states. The continuous phenotypic shifts in T cells could be inferred from CyTOF data, and transfer learning enabled estimation of patient-specific cell states in new sample cohorts.
Article
Urology & Nephrology
Lola Jacquemont, Gaelle Tilly, Michelle Yap, Tra-My Doan-Ngoc, Richard Danger, Pierrick Guerif, Florent Delbos, Bernard Martinet, Magali Giral, Yohann Foucher, Sophie Brouard, Nicolas Degauque
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2020)
Article
Immunology
Florian Dubois, Sophie Limou, Melanie Chesneau, Nicolas Degauque, Sophie Brouard, Richard Danger
EUROPEAN JOURNAL OF IMMUNOLOGY
(2020)
Article
Immunology
Melanie Chesneau, Hoa Le Mai, Richard Danger, Sabine Le Bot, Thi-Van-Ha Nguyen, Josselin Bernard, Cyrielle Poullaouec, Pierrick Guerrif, Sophie Conchon, Magali Giral, Beatrice Charreau, Nicolas Degauque, Sophie Brouard
JOURNAL OF IMMUNOLOGY
(2020)
Article
Medicine, Research & Experimental
Benjamin Gaborit, Bernard Vanhove, Marie-Anne Vibet, Aurelie Le Thuaut, Karine Lacombe, Vincent Dubee, Florence Ader, Virginie Ferre, Eric Vicaut, Jeremie Orain, Morgane Le Bras, Anne Omnes, Laetitia Berly, Alexandra Jobert, Pascale Morineau-Le Houssine, Karine Botturi, Regis Josien, Laurent Flet, Nicolas Degauque, Sophie Brouard, Odile Duvaux, Alexandra Poinas, Francois Raffi
Summary: Early inhibition of SARS-CoV-2 entry and replication using polyclonal neutralizing antibodies shows promising therapeutic potential by providing immediate immunity and blocking viral infection in ACE-2-positive human cells. The study aims to determine the safety, optimal dose, and clinical benefits of XAV-19 in patients with COVID-19-induced moderate pneumonia, with the goal of potentially introducing it as a new therapeutic option in combination with antiviral and immunotherapies.
Review
Biochemistry & Molecular Biology
Nicolas Degauque, Alain Haziot, Sophie Brouard, Nuala Mooney
Summary: In the inflammation and infection caused by SARS-CoV-2, endothelial cells play a crucial role in regulating the recruitment of immune cells and interacting with monocytes, T cells, and B cells. Endothelial cells function as an integrative and active platform for immune responses, providing opportunities for therapeutic intervention.
Review
Medicine, General & Internal
A. Neel, N. Degauque, S. Bruneau, C. Braudeau, M. Bucchia, A. Caristan, D. De Mornac, V Genin, A. Glemain, C. Oriot, M. Rimbert, S. Brouard, R. Josien, M. Hamidou
Summary: AAV is a group of rare systemic immune-mediated diseases characterized by small vessel necrotizing vasculitis and respiratory tract inflammation. Research in animal models and in humans has identified the roles of ANCA, neutrophils, T helper cells, B cells, complement activation, and other factors in the pathogenesis of AAV. New investigation tools may lead to a better understanding of the disease mechanisms and potential therapeutic targets.
REVUE DE MEDECINE INTERNE
(2022)
Article
Immunology
Gaelle Tilly, Marion Cadoux, Alexandra Garcia, Jeremy Morille, Sandrine Wiertlewski, Claire Pecqueur, Sophie Brouard, David Laplaud, Nicolas Degauque
Summary: The study found that 12 months of treatment with teriflunomide in patients with RRMS did not affect B cell or CD4 T cell compartments, but had a specific impact on CD8 T cells, including decreased proliferation and reduced production of inflammatory factors. DHODH inhibition also affected the migratory velocity of memory CD8 T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Surgery
Jesper Hjortdal, Matthew D. Griffin, Marion Cadoux, W. John Armitage, Max Bylesjo, Peadar Mac Gabhann, Conor C. Murphy, Uwe Pleyer, Derek Tole, Bertrand Vabres, Malcolm D. Walkinshaw, Pierre-Antoine Gourraud, Matilde Karakachoff, Sophie Brouard, Nicolas Degauque
Summary: This study investigated the acute rejection of corneal transplants and found that there were no activation signatures within the B cell and T cell repertoire at the time of diagnosis. However, in recipients of full-thickness corneal transplants, acute rejection was associated with differences in B cell maturity and regulatory CD4(+) T cell frequency compared to stable allografts.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Urology & Nephrology
Tra-My Doan Ngoc, Gaelle Tilly, Richard Danger, Orianne Bonizec, Christophe Masset, Pierrick Guerif, Sarah Bruneau, Alexandre Glemain, Jean Harb, Marion Cadoux, Anais Vivet, Hoa Le Mai, Alexandra Garcia, David Laplaud, Roland Liblau, Magali Giral, Stephanie Blandin, Magalie Feyeux, Laurence Dubreuil, Claire Pecqueur, Matthew Cyr, Weiming Ni, Sophie Brouard, Nicolas Degauque
Summary: Our study reveals that humoral rejection of kidney transplants is associated with the accumulation of TEMRA CD8(+) T cells in blood and kidney graft biopsies. TEMRA CD8(+) T cells from kidney transplant recipients show enhanced migratory properties compared to EM CD8(+) T cells, with increased adhesion to activated endothelium and transmigration in response to CXCL12 chemokine. Furthermore, IL-15 stimulation promotes the migration of TEMRA and EM CD8(+) T cells induced by CXCL12 and enhances the generation of functional PSGL1, which interacts with P-selectin and facilitates adhesion to activated endothelium. Notably, inhibiting the migration of TEMRA CD8(+) T cells from kidney transplant recipients by targeting VLA-4 or LFA-1 suggests the potential therapeutic benefit of targeting these cells in transplant recipients.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Medicine, General & Internal
Hoa Le Mai, Nicolas Degauque, Sabine Le Bot, Marie Rimbert, Karine Renaudin, Richard Danger, Florent Le Borgne, Clarisse Kerleau, Gaelle Tilly, Anais Vivet, Florent Delbos, Alexandre Walencik, Magali Giral, Sophie Brouard
Summary: The study found that the percentage and absolute number of CD28-CD8+ T cells were significantly increased in kidney transplant patients with antibody-mediated rejection (ABMR). Moreover, CD28-CD8+ T cells from patients with ABMR showed a more rigorous response to stimulation compared to their CD28+ counterparts. These findings suggest that differentiated CD28-CD8+ T cells, with increased frequency, number, and function, may play a role in the pathobiology of ABMR.
Article
Immunology
Hoa Le Mai, Nicolas Degauque, Marine Lorent, Marie Rimbert, Karine Renaudin, Richard Danger, Clarisse Kerleau, Gaelle Tilly, Anais Vivet, Sabine Le Bot, Florent Delbos, Alexandre Walencik, Magali Giral, Sophie Brouard
Summary: This study found that kidney allograft rejection is associated with an imbalance between immune cells with effector/memory functions and those with regulatory properties, manifested as a decrease in the percentage of circulating B cells and an increase in the ratio of CD28-CD8+ T cells to Tregs.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Tess Van Meerhaeghe, Antoine Neel, Sophie Brouard, Nicolas Degauque
Summary: The role of B cells in the regulation of CD8 T cell survival, proliferation, and memory formation has been a topic of debate. B cells can act as antigen-presenting cells for CD8 T cells due to their high expression of MHC class I molecules. In various conditions, such as viral infections, autoimmune diseases, cancer, and allograft rejection, B cells have been shown to modulate CD8 T cell function. However, B-cell depletion therapies can impair CD8 T cell responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Meeting Abstract
Surgery
H. L. Mai, N. Degauque, S. Le Bot, M. Rimbert, K. Renaudin, R. Danger, F. Le Borgne, C. Kerleau, F. Delbos, M. Giral, S. Brouard
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Meeting Abstract
Surgery
Gaelle Tilly, Tra-My Doan-Ngoc, Orianne Bonizec, Christophe Masset, Richard Danger, Pierrick Guerif, Sarah Bruneau, Alexandre Glemain, Jean Harb, Magali Giral, Claire Pecqueur, Sophie Brouard, Nicolas Degauque
TRANSPLANT INTERNATIONAL
(2021)
Meeting Abstract
Surgery
Hoa Mai, Sabine Le Bot, Marie Rimbert, Karine Autain-Renaudin, Richard Danger, Florent Leborgne, Clarisse Kerleau, Nicolas Degauque, Florent Delbos, Magali Giral, Sophie Brouard
TRANSPLANT INTERNATIONAL
(2021)
