Article
Microbiology
Preeti Sharma, Sumanpreet Kaur, Bhupinder Singh Chadha, Raminderjit Kaur, Manpreet Kaur, Sukhraj Kaur
Summary: Enterocin 12a is a novel bacteriocin with anticancer properties against human cancer cell lines. It selectively inhibits the proliferation of cancer cells in a dose-dependent manner and induces apoptosis-like morphological changes, while having negligible activity towards non-malignant cells. Further evaluation in animal models is recommended to explore its potential as an anticancer agent.
Article
Veterinary Sciences
Huseyin Ozturk, Burak Genis, Banu Ozden Tuncer, Yasin Tuncer
Summary: This study aimed to isolate bacteriocin-producing lactic acid bacteria (LAB) from sheep and goat colostrum and characterize their properties. Thirteen bacteriocin-producing LAB strains were isolated, identified as Enterococcus mundtii and Enterococcus faecium, and found to be genetically diverse. The bacteriocins showed activity in a wide pH range, were heat resistant, but sensitive to proteolytic enzymes and alpha-amylase, and exhibited resistance to detergents. Some strains contained multiple enterocin genes. Different strains were identified as producers of specific types of bacteriocins. The strains also exhibited various metabolic enzyme activities, but lacked extracellular proteolytic and lipolytic activities.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Applied
Neelima Sharma, Rajan Sharma, Yudhishthir S. Rajput, Bimlesh Mann, Kamal Gandhi
Summary: A new method has been developed for the detection of glycomacropeptide and beta-lactoglobulin in cheese whey, using cationic 'stains all' dye after tricine SDS-PAGE, overcoming the poor sensitivity of common staining dye 'coomassie brilliant blue' towards glycomacropeptide.
Article
Microbiology
Charikleia Tsanasidou, Stamatia Asimakoula, Nikoletta Sameli, Christos Fanitsios, Elpiniki Vandera, Loulouda Bosnea, Anna-Irini Koukkou, John Samelis
Summary: Autochthonous enterocin-producing strains of dairy enterococci show potential for use as bioprotective adjunct cultures in traditional cheese technologies, with most strains being safe, resistant to certain antibiotics, and exhibiting enzymatic activities. These strains, except for one, are considered safe for use in Greek cheeses, but further in situ biotechnological evaluations are needed for confirmation.
Article
Food Science & Technology
Mina Nasiri, Shahram Hanifian
Summary: The study assessed the prevalence of enterococci in pasteurized milk samples from retail stores in Northwest Iran, finding that most isolates were identified as E. faecalis or E. faecium, carrying various virulence genes and showing high resistance to most antibiotics tested. Additionally, the isolates were able to produce biofilm, suggesting that pasteurized milk could potentially transmit multidrug-resistant virulent enterococci strains to humans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Immunology
Zhi-lin Liu, Yun-jiao Chen, Qing-lei Meng, Xin Zhang, Xue-li Wang
Summary: As a probiotic, enterococcus faecium (E. faecium) has been widely used in the livestock industry due to its high temperature resistance, gastric acid resistance, bile salt resistance, and the ability to improve animal performance, immunity, and intestinal environment. However, the improper use of antibiotics and the environmental stress of strains have led to serious drug resistance and safety concerns, as some pootentially pathogenic strains of enterococcus faecium carry virulence genes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Kenji Kudo, Naoyuki Tsukamoto, Masahiko Ito, Nobumichi Kobayashi
Summary: This study investigated the prevalence of virulence factors, drug resistance and its genetic determinants, and clonal lineages of E. faecalis and E. faecium clinical isolates in northern Japan. The results showed that E. faecalis isolates had a higher prevalence of virulence factors promoting bacterial colonization compared to E. faecium. There were evident differences in resistance to certain antibiotics and its determinants between the two species. Additionally, the first identification of optrA gene in ST480 E. faecalis in Japan was reported.
Article
Biotechnology & Applied Microbiology
Samantha Joy D. Valledor, Clarizza May Dioso, Jorge Enrique Vazquez Bucheli, Yu Jin Park, Dong Ho Suh, Eun Sung Jung, Bobae Kim, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: Enterococcus faecium ST20Kc and ST41Kc isolated from kimchi produce bacteriocins with strong activity against Listeria monocytogenes and various Enterococcus spp. The antimicrobials are proteinaceous and stable, with a bactericidal effect on targeted bacterial strains. Both strains are susceptible to antibiotics and potentially produce additional antimicrobial compounds with metabolomics analysis.
Article
Food Science & Technology
Renpeng Du, Wenxiang Ping, Jingping Ge
Summary: The novel bacteriocin enterocin HDX-2 exhibited high stability and strong bactericidal activity against a variety of bacteria, with mechanisms such as cell lysis, alterations in cell morphology and intracellular activities, and induction of K+ and inorganic phosphate leakage in bacteria.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Muzamil Rashid, Sunil Sharma, Arvinder Kaur, Amarjeet Kaur, Sukhraj Kaur
Summary: The increasing awareness of the negative health effects of synthetic preservatives has led to a higher demand for safe food preservation techniques and bio preservatives. This study evaluated the effectiveness of the biopreservative Enterococcus faecium Smr18 and its enterocin ESmr18 against Salmonella enterica contamination in chicken samples. The enterocin ESmr18 showed broad-spectrum antibacterial activity and did not cause any cytolytic effect on human red blood cells.
Article
Multidisciplinary Sciences
Thomas F. Oftedal, Dzung B. Diep
Summary: A cost-effective and rapid method for detecting Enterococcus faecium in urine using a fluorescently labelled bacteriocin was developed. This method showed an increase in fluorescent signals of 25-73-fold compared to control samples containing Escherichia coli or Staphylococcus aureus. Bacteriocins have the potential to act as specific probes for detecting specific bacteria in biological samples.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Yunshu Feng, Nan Yin, Zhijiang Zhou, Ye Han
Summary: A composite film named BC-E was prepared using bacterial cellulose (BC) of Gluconacetobacter xylinus and cellfree supernatant (CFS) of Enterococcus faecium TJUQ1, showing significantly improved mechanical properties compared to BC. The BC-E film exhibited higher tensile strength, elongation at break, and water resistance than BC, and effectively reduced the number of Listeria monocytogenes and total mesophilic bacteria in ground meat during storage.
Article
Biotechnology & Applied Microbiology
S. Lengliz, M. S. Abbassi, A. Rehaiem, N. Ben Chehida, T. Najar
Summary: This study identified potential probiotic lactococcus bacteria from rabbits, with one Enterococcus durans isolate showing promising application prospects in rabbit husbandry. The isolates also displayed relevant resistance phenotypes and virulence factors, highlighting their potential as autochthonous probiotics.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Edibe Rabia Ozkan, Talha Demirci, Nihat Akin
Summary: Nine Enterococcus faecium isolates from Tulum cheese in Turkey were analyzed for probiotic potential through various tests, with one isolate showing promising probiotic properties and deemed worthy of further in vivo investigation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Nashwa Tarek, Ahmed F. Azmy, Ahmed S. Khairalla, Medhat Abdel-Fattah, Ohoud A. Jefri, Mohamed Shaban, Abdelaziz A. A. El-Sayed, Ahmed O. El-Gendy
Summary: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. The bacterium NT04 was identified as Enterococcus faecium through 16S rRNA gene sequencing. Genomic analysis confirmed the presence of genes encoding diverse metabolic and probiotic properties in strain NT04. No pathogenicity islands or plasmid insertions were found. The genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
Article
Biotechnology & Applied Microbiology
Ozge Yuceer, Banu Ozden Tuncer
JOURNAL OF FOOD SAFETY
(2015)
Article
Polymer Science
Cigdem Karabacak Atay, Merve Gokalp, Banu Ozden Tuncer, Tahir Tilki
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
(2017)
Article
Microbiology
Mine Avci, Banu Ozden Tuncer
POLISH JOURNAL OF MICROBIOLOGY
(2017)
Article
Food Science & Technology
B. Ozden, M. Akcelik
Article
Biology
Nefise Akkoc, Banu Ozden, Beguem G. Tan, Mustafa Akcelik
Article
Chemistry, Multidisciplinary
Cigdem Karabacak, Tahir Tilki, Banu Ozden Tuncer, Mustafa Cengiz
RESEARCH ON CHEMICAL INTERMEDIATES
(2015)
Article
Biology
Bann Ozden Tuncer, Zeliha Ay, Yasin Tuncer
TURKISH JOURNAL OF BIOLOGY
(2013)
Article
Food Science & Technology
Banu Ozden Tuncer, Yasin Tuncer
JOURNAL OF FOOD AND NUTRITION RESEARCH
(2011)
Article
Food Science & Technology
Burcu Ozkalp, Banu Ozden, Yasin Tuncer, Pinar Sanlibaba, Mustafa Akcelik
Article
Biology
Cagla Tukel, Pinar Sanlibaba, Banu Ozden, M. Akcelik
ACTA BIOLOGICA HUNGARICA
(2006)
Article
Biotechnology & Applied Microbiology
Yasin Tuncer, Banu Ozden
ROMANIAN BIOTECHNOLOGICAL LETTERS
(2010)
Article
Biology
Mehtap Uestuen, Banu Oezden, Oemer Simsek, Mustafa Akcelik
TURKISH JOURNAL OF BIOLOGY
(2009)