期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 123, 期 -, 页码 261-268出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.11.049
关键词
Omega-3 polyunsaturated fatty acid; Selective hydrolysis; Molecular docking; Regioselectivity; Lipase
资金
- K.C. Wong Magna Fund in Ningbo University
- National Natural Science Foundation of China [21706137]
- Ningbo University [421401560, 421793030]
- Regional Demonstration Project of Marine Economic Innovation and Development
Present study employed molecular modeling method to elucidate the binding affinity of lipases with fatty acids of different chain lengths; and investigated the effects of lipases positional and fatty acids specificity on omega-3 polyunsaturated fatty acids (omega-3 PUFAs) enrichment in cod liver and linseed oils. Among the lipases studied, molecular modeling showed the active sites of Candida rugosa lipase (CRL) had a low C-Docker interactive energy for saturated (SFA) and monounsaturated (MUFA) fatty acids which predicted CRL to have highest preferences to selectively hydrolyze resulting in efficient enrichment of omega-3 PUFAs. Verification experiments showed the SFA and MUFA in the acylglycerol fraction includes monoacylglcyerols (MAG), diacyglycerols (DAG), and triacylglycerols (TAG) of CRL-hydrolyzed cod liver oil decreased from the initial 25.21 to 16.88% and 45.25 to 32.17%, respectively. In addition, CRL-hydrolyzed cod liver oil demonstrated 88.36% of omega-3 PUFAs enrichment. The regio-distribution of fatty acids in CRL-hydrolyzed cod liver oil were not significantly different than that of cod liver oil indicating the omega-3 PUFAs enrichment was due to fatty acids selectivity and not positional selectivity of CRL. (C) 2018 Elsevier B.V. All rights reserved.
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