期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 124, 期 -, 页码 50-59出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.11.104
关键词
Padina gymnospora; Sulfated polysaccharide; Oxidative stress; Antioxidants; Reactive oxygen species
资金
- Atomic Energy Regulatory Board [60/08/2015]
In this present study, isolation, characterization and protective effect of sulfated polysaccharide (SP) isolated from the brown algae Padina gymnospora was investigated. SP was isolated and characterized through FT-IR, H-1 NMR, TGA, GC-MS and CHN analysis. The molecular weight of SP was found to be 16 kDa. The isolated SP contains 29.4 +/- 035% of sulfate, 27 +/- 0.11% of fucose, 0.05 +/- 0.12% of protein, respectively. Furthermore, SP exhibits its excellent radical scavenging effects were evaluated by DPPH, ABTS radical scavenging and reducing power assays. Moreover, pretreatment with SP significantly mitigates H2O2 induced cytotoxicity in L-929 cells in a dose dependent manner. Furthermore, SP pretreatment ameliorates oxidative stress induced apoptosis and DNA damage, alleviates the generation of intracellular reactive oxygen species (ROS) and restores mitochondrial membrane potential (MMP) in L-929 cells through its antioxidant potential. Together, these results suggest that SP can be exploited as a natural antioxidant in the food and pharmaceutical industries. (C) 2018 Elsevier B.V. All rights reserved.
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