期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 54, 期 1, 页码 75-79出版社
ELSEVIER
DOI: 10.1016/j.ijantimicag.2019.03.012
关键词
Chloramphenicol acetyltransferase; Intrinsic resistance; Naturally occurring variant; Vibrio parahaemolyticus
资金
- National Natural Science Foundation of China [3150 0 061]
- National Key Basic Research Development Plan of China (973 Program) [2015CB554202]
- Sanming Project of Medicine in Shenzhen [SZSM201811071]
Intrinsic resistance of bacteria to antibiotics plays an increasingly significant role in antibiotic resistance. However, the breadth of intrinsic resistance has not been fully elucidated. Here we identified a novel class of chloramphenicol acetyltransferase (type C CAT or CATC) in Vibrio parahaemolyticus and its closely related species V. alginolyticus, V. antiquarius, and V. diabolicus. The catC genes encoding the CATC clade are distributed among the four Vibrio species and are consistently found in the same conserved genomic regions. Based on their prevalence, these genes are considered to be intrinsic in V. parahaemolyticus, V. alginolyticus, V. antiquarius, and V. diabolicus. We also demonstrated that naturally occurring variants of CATC can confer diverse resistance levels against chloramphenicol in Escherichia coli. Furthermore, the enzyme kinetics of CATC variant proteins supported the diversity of their resistance phenotypes. This work provides insights into the distribution and resistance phenotypes of a novel class of intrinsic resistance genes in bacteria. (C) 2019 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
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