期刊
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
卷 193, 期 1, 页码 78-85出版社
AMER THORACIC SOC
DOI: 10.1164/rccm.201504-0733OC
关键词
cytomegalovirus; lung transplantation; immunologic monitoring
资金
- Biomarker Factory, LLC (a Duke University/LabCorp joint company)
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR001117]
Rationale: Cytomegalovirus (CMV), which is one of the most common infections after lung transplantation, is associated with chronic lung allograft dysfunction and worse post-transplantation survival. Current approaches for at-risk patients include a fixed duration of antiviral prophylaxis despite the associated cost and side effects. Objectives: We sought to identify a specific immunologic signature that predicted protection from subsequent CMV. Methods: CMV-seropositive lung transplantation recipients were included in the discovery (n = 43) and validation (n = 28) cohorts. Polyfunctional CMV-specific immunity was assessed by stimulating peripheral blood mononuclear cells with CMV pp65 or IE-1 peptide pools and then by measuring T-cell expression of CD107a, IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and IL-2. Recipients were prospectively monitored for subsequent viremia. A Cox proportional hazards regression model that considered cytokine responses individually and in combination was used to create a predictive model for protection from CMV reactivation. This model was then applied to the validation cohort. Measurements and Main Results: Using the discovery cohort, we identified a specific combination of polyfunctional T-cell subsets to pp65 that predicted protection from subsequent CMV viremia (concordance index 0.88 [SE, 0.087]). The model included both protective (CD107a(-)/IFN-gamma(+)/IL-2(+)/TNF-alpha(+) CD4(+) T cells, CD107a(-)/IFN-gamma(+)/IL-2(+)/TNF-alpha(+) CD8(+) T cells) and detrimental (CD107a(+)/IFN-gamma(+)/IL-2(-)/TNF-alpha(-) CD8(+) T cells) subsets. The model was robust in the validation cohort (concordance index 0.81 [SE, 0.103]). Conclusions: We identified and validated a specific T-cell polyfunctional response to CMV antigen stimulation that provides a clinically useful prediction of subsequent cytomegalovirus risk. This novel diagnostic approach could inform the optimal duration of individual prophylaxis.
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