期刊
FOOD CHEMISTRY
卷 276, 期 -, 页码 707-713出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.10.047
关键词
Monoclonal antibody; Immunochromatographic assay; Toltrazuril; Metabolite; Feed; Egg; Chicken
资金
- Natural Science Foundation of Jiangsu Province
- MOE [BX20151038, BK20180029, BK20180605, BE2016307, BK20140003, BE2013613, BE2013611]
- MOF
In this study, a sensitive monoclonal antibody (mAb) against toltrazuril (Tol) was developed based on a novel hapten. The 50% inhibitory concentrations (IC50) of toltrazuril and its metabolites ranged from 2.19 ng/mL to 4.21 ng/mL. Based on this mAb, a colorimetric paper-based sensor was developed for the rapid screening of Tol and its metabolites in samples. The proposed assay has cutoff values of < 20 mu g/kg for Tol and 50 mu g/kg for Tol sulfone when evaluated with the naked eye, and the results could be obtained in 15 min. Quantitative results were obtained with a strip scan reader, with limits of detection < 2.60 mu g/kg for Tol and its metabolites in real samples. The sensitivity of both qualitative and quantitative detection meets the European Union requirements. Therefore, this strip assay provides a useful tool for the on-site detection and rapid initial screening of Tol and its derivatives in feed, egg, and chicken samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据