期刊
EXPERIMENTAL AND MOLECULAR PATHOLOGY
卷 106, 期 -, 页码 78-88出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2018.11.013
关键词
Endometrial carcinoma; Cancer stem cell; SAGE-Seq; Tumorigenicity; Chemo-resistance
类别
资金
- KAKENHI [15H04722, 171101540]
- Japan Agency for Medical Research and Development, AMED
- Ono Cancer Research Fund
- Northern Advancement Center for Science & Technology of Hokkaido Japan
- Grants-in-Aid for Scientific Research [15H04722] Funding Source: KAKEN
Uterine endometrial carcinoma is one of the common cancers in females. Cancer stem-like cells (CSCs)/cancerinitiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. However, the heterogeneity of CSCs/CICs is still elusive, and we therefore analyzed CSCs/CICs at the clonal level. We previously established sphere-cultured CSCs/CICs from primary human uterine endometrial carcinoma, and we isolated several clones from CSCs/CICs in this study. Interestingly, we established two types of clones based on the growth pattern. The clones were termed sphere clones (S clones) and leukemia-like clones (LL clones). Functional analysis revealed that S clones are resistant to chemotherapy, whereas LL clones are sensitive to chemotherapy. On the other hand, S clones are less tumorigenic, while LL clones are highly tumorigenic. Transcriptome analysis using serial analysis of gene expression sequencing (SAGE-Seq) revealed distinctive gene expression profiles in S clone cells and LL clone cells. The results indicate that CSCs/CICs are composed of functionally heterogenic subpopulations including highly tumorigenic clones and treatment-resistant clones and that the characteristics of CSCs/CICs might be determined by the characteristics of different clones that compose CSCs/CICs.
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