期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 311, 期 2, 页码 L375-L388出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00132.2016
关键词
sugen/hypoxia; fibrosis; apoptosis; endothelial nitric oxide synthase; autophagy
资金
- Indiana University Health-Indiana University School of Medicine Strategic Research Initiative
- Gilead Sciences Research Scholars Program in PAH
- VA Merit [1I01BX002042-01A2]
- National Institutes of Health (NIH) [5TL1TR001107-02, NIH R01HL086939]
- American Heart Association Midwest Affiliates Scientist Development Grant [13SDG17140066]
17 beta-Estradiol (E-2) exerts protective effects on right ventricular (RV) function in pulmonary arterial hypertension (PAH). Since acute exercise-induced increases in afterload may lead to RV dysfunction in PAH, we sought to determine whether E-2 allows for superior RV adaptation after an acute exercise challenge. We studied echocardiographic, hemodynamic, structural, and biochemical markers of RV function in male and female rats with sugen/hypoxia (SuHx)-induced pulmonary hypertension, as well as in ovariectomized (OVX) SuHx females, with or without concomitant E-2 repletion (75 mu g.kg(-1).day(-1)) immediately after 45 min of treadmill running at 75% of individually determined maximal aerobic capacity (75% aerobic capacity reserve). Compared with males, intact female rats exhibited higher stroke volume and cardiac indexes, a strong trend for better RV compliance, and less pronounced increases in indexed total pulmonary resistance. OVX abrogated favorable RV adaptations, whereas E-2 repletion after OVX markedly improved RV function. E-2's effects on pulmonary vascular remodeling were complex and less robust than its RV effects. Postexercise hemodynamics in females with endogenous or exogenous E-2 were similar to hemodynamics in nonexercised controls, whereas OVX rats exhibited more severely altered postexercise hemodynamics. E-2 mediated inhibitory effects on RV fibrosis and attenuated increases in RV collagen I/III ratio. Proapoptotic signaling, endothelial nitric oxide synthase phosphorylation, and autophagic flux markers were affected by E-2 depletion and/or repletion. Markers of impaired autophagic flux correlated with endpoints of RV structure and function. Endogenous and exogenous E-2 exerts protective effects on RV function measured immediately after an acute exercise challenge. Harnessing E-2's mechanisms may lead to novel RV-directed therapies.
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