期刊
ELECTROCHEMISTRY COMMUNICATIONS
卷 99, 期 -, 页码 51-55出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.elecom.2019.01.003
关键词
DNA walker; DNA star trigon; Nicking endonuclease; Square wave voltammetry; Signal amplification
资金
- National Natural Science Foundation of China [81771929]
- Scientific Research Instrument Developing Project of the Chinese Academy of Sciences [YJKYYQ20170067]
Synthetic artificial DNA probes are excellent architectural scaffolds which can be assembled into various nanostructures for further applications. A DNA walker is usually designed in response to certain external stimuli, and can significantly enhance the obtained signals. Herein, we have developed a novel DNA walker biosensor for the detection of target DNA with enzymatic recycling cleavage. By using the target DNA as a driving force, a DNA star trigon nanostructure is formed on the electrode surface, which can be recognized by a nicking endonuclease. The DNA walker is then activated to move around for the cleavage reactions on the electrode. The resulting DNA biosensor shows excellent analytical performance with a low limit of detection of 1 fM. Good selectivity and practical utility are also demonstrated. This approach has many other advantages such as ease of use, low cost, mild conditions, and may hold great potential for a range of applications in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据