Article
Chemistry, Analytical
Jie Zhang, Zengchen Liu, Fengshou Tian, Yahong Chen
Summary: A fluorescent compound derived from coumarin and hemicyanine was synthesized and characterized in this paper, showing higher ratiometric fluorescence response activity toward NAD(P)H compared to other commonly coexisting compounds in the cell microenvironment. The fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H. This ratiometric fluorescent probe provides a theoretical basis for sensing NAD(P)H in vitro and in vivo, and was used to build a sensitive detection platform of NAD(P)H-dependent enzyme activity based on the fluorescence method.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Yongwoo Jang, Taegyu Park, Eunyoung Kim, Jong Woo Park, Dong Yeop Lee, Seon Jeong Kim
Summary: By utilizing the cellular redox system, a CNT yarn supercapacitor with maximum area capacitance was developed and demonstrated excellent performance under physiological conditions. Implanted NAD/BQ/CNT yarn electrodes in a rat's abdominal cavity showed stable in vivo electrical performance of a supercapacitor. These findings suggest a promising platform for implantable energy storage devices using redox biomolecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Wenyi Qin, Ann Jeffers, Shuzi Owens, Prashant Chauhan, Satoshi Komatsu, Guoqing Qian, Xia Guo, Mitsuo Ikebe, Steven Idell, Torry A. Tucker
Summary: Research has shown that NOX1 and NOX4 are critical determinants of MesoMT, and downregulation of NOX1 and NOX4 play important roles in blocking THB- and Xa-mediated MesoMT.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Hematology
Jessica Venugopal, Jintao Wang, Chiao Guo, Daniel T. Eitzman
Summary: Pharmacological IL-1R antagonism improves anaemia in a murine SCD model. IL-1 beta stimulation of platelets promotes erythrocyte sickling, possibly mediated by platelet-derived TGF-beta-induced reactive oxygen species generation through erythrocyte NADPH oxidase.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stefania Olla, Chiara Siguri, Antonella Fais, Benedetta Era, Massimo Claudio Fantini, Amalia Di Petrillo
Summary: This study investigates the mechanisms underlying the antioxidant properties of quercetin and its ability to target endogenous oxidant enzymes. The results demonstrate that quercetin acts as a free radical scavenger by donating electrons and effectively inhibits the activity of various oxidative enzymes by binding to them with high affinity. Molecular docking and subsequent molecular dynamics confirm the binding of quercetin to these enzymes, with different stability phases observed depending on the enzyme bound. The findings suggest that quercetin has potential as a natural therapy for diseases related to oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Nutrition & Dietetics
Andrea Benzi, Alessia Grozio, Sonia Spinelli, Laura Sturla, Andreas H. Guse, Antonio De Flora, Elena Zocchi, Joerg Heeren, Santina Bruzzone
Summary: Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in energy metabolism regulation as both a coenzyme and substrate for various enzymes. CD38, as an enzyme synthesizing Ca2+-mobilizing second messenger and a major NAD(+)-consumer, can impact NAD(+) levels. This review explores recent studies on the modulation of CD38 expression and activity, and the resulting changes in NAD(P)(H) levels in adipose tissue.
Article
Biochemistry & Molecular Biology
Cuiting Liao, Li Zhang, Rong Jiang, Juanjuan Xu, Jiarui Tang, Kai Hu, Shifang Jiang, Longhui Li, Yongqiang Yang, Jiayi Huang, Li Tang, Longjiang Li
Summary: The expression of NADK is positively correlated with APAP-induced acute liver injury, and inhibition of NADK can alleviate liver injury by increasing hepatic NAD(+) level and improving antioxidant capacity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Neurosciences
Mariajose Metcalfe, Brian T. David, Brett C. Langley, Caitlin E. Hill
Summary: Spinal cord injury leads to tissue damage and functional deficits. Administering NR can increase NAD+ levels in the injured spinal cord, protect tissue, and improve motor function.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Chemistry, Physical
Hao Wang, Jinxing Chen, Qing Dong, Xu Sun, Qiong Liu, Dan Li, Erkang Wang, Jin Wang
Summary: Most nanozyme research focuses on oxidase and peroxidase capabilities, but this study explores N, P, or S doped carbon nanotubes (CNTs) as enzyme mimics of NADH oxidase and Cyt c reductase. Doping N significantly enhances the NADH oxidase-like activity of CNTs, increasing the maximum initial velocity by 4.28 times. NAD(+) is produced as a biologically active product during NADH oxidation, while oxygen is selectively reduced to water or hydrogen peroxide, similar to natural NADH oxidase. Additionally, carbon nanotubes can promote electron transfer from NADH to Cyt c, mimicking the properties of Cyt c reductase.
Review
Pharmacology & Pharmacy
Yumeng Zhu, Ping Xu, Xuan Huang, Wen Shuai, Li Liu, Shuai Zhang, Rui Zhao, Xiuying Hu, Guan Wang
Summary: Nicotinamide phosphoribosyltransferase (NAMPT) plays a significant role in mammals by synthesizing nicotinamide mononucleotide (NMN) for metabolic homeostasis and cell survival, serving as a key protein in the host's defense mechanism. Neurodegenerative diseases pose a serious threat to human health, but effective drugs are limited. NAMPT has active biological functions in neurogenesis, making it a powerful therapeutic target for neurodegenerative diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Nutrition & Dietetics
Qin Song, Xiaofeng Zhou, Kexin Xu, Sishi Liu, Xinqiang Zhu, Jun Yang
Summary: Nicotinamide mononucleotide (NMN) is a precursor for NAD+ biosynthesis, and supplementation has shown to increase NAD+ concentration and alleviate aging-related disorders. While most studies have been conducted in cell or animal models, several human clinical trials are currently evaluating the safety and physiological effects of NMN supplementation.
ADVANCES IN NUTRITION
(2023)
Article
Multidisciplinary Sciences
JuKyung Lee, Han Na Suh, Saeyoung Ahn, Hye Bin Park, Jeong Yoon Lee, Hyung Jin Kim, Sang Hee Kim
Summary: This study developed electrocatalytic sensors for analyzing NADH in whole blood, which exhibited stability and a limit of detection of 3.5 μM. These sensors have the potential for extensive applications.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Yu-jie Xing, Biao-hu Liu, Shu-jun Wan, Yi Cheng, Si-min Zhou, Yue Sun, Xin-ming Yao, Qiang Hua, Xiang-jian Meng, Jin-han Cheng, Min Zhong, Yan Zhang, Kun Lv, Xiang Kong
Summary: Dap protects cardiac myocytes from damage caused by hyperglycemia by suppressing NADPH oxidase-mediated oxidative stress, improving cardiac dysfunction and reducing myocardial fibrosis and apoptosis levels.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Ryo Matsuyama, Tomoyo Omata, Michiharu Kageyama, Ryota Nakajima, Masanobu Kanou, Kei Yamana
Summary: Nicotinamide adenine dinucleotide (NAD(+)) is an essential coenzyme for energy production. Associations between NAD(+) and aging-related diseases have been reported, and NAD(+) precursors are acknowledged as anti-aging supplements. However, there have been few studies on the link between aging or aging-related diseases and human blood NAD(+) concentration due to the instability and difficulty in measuring NAD(+). This study aimed to develop a simpler quantitative NAD(+) measurement method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yan Liu, Chengting Luo, Ting Li, Wenhao Zhang, Zhaoyun Zong, Xiaohui Liu, Haiteng Deng
Summary: The study demonstrates that NMNH is a potent enhancer of NAD(+) and suppresses glycolysis, the TCA cycle, and cell growth. NMNH, produced through a chemical method, is more effective than NMN in increasing NAD(+) levels in vivo and in vitro, mediated by NMNAT.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Critical Care Medicine
H. Ibrahim Korkmaz, Magda M. W. Ulrich, Gulbahar Celik, Wessel N. Van Wieringen, Paul P. M. Van Zuijlen, Paul A. J. Krijnen, Hans W. M. Niessen
JOURNAL OF BURN CARE & RESEARCH
(2020)
Article
Hematology
Guus A. de Waard, Maurits R. Hollander, Danique Ruiter, Thomas ten Bokkel Huinink, Romain Meer, Nina W. van der Hoeven, Elisa Meinster, Jeroen A. M. Belien, Hans W. Niessen, Niels van Royen
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)
Review
Endocrinology & Metabolism
Sanne J. van der Veen, Carla E. M. Hollak, Andre B. P. van Kuilenburg, Mirjam Langeveld
JOURNAL OF INHERITED METABOLIC DISEASE
(2020)
Article
Immunology
Liza Rijvers, Marie-Jose Melief, Jamie van Langelaar, Roos M. van der Vuurst de Vries, Annet F. Wierenga-Wolf, Steven C. Koetzier, John J. Priatel, Tineke Jorritsma, S. Marieke van Ham, Rogier Q. Hintzen, Marvin M. van Luijn
JOURNAL OF IMMUNOLOGY
(2020)
Article
Genetics & Heredity
Sanna Puusepp, Karit Reinson, Sander Pajusalu, Andre B. P. van Kuilenburg, Doreen Dobritzsch, Jeroen Roelofsen, Werner Stenzel, Katrin Ounap
MOLECULAR GENETICS AND METABOLISM REPORTS
(2020)
Article
Oncology
Jatta Saarenheimo, Nesna Wahid, Natalja Eigeliene, Ravichandra Ravi, Gajja S. Salomons, Matilde Fernandez Ojeda, Raymon Vijzelaar, Antti Jekunen, Andre B. P. van Kuilenburg
Summary: This study describes the application of upfront DPD screening in Finnish patients, revealing that 8% of patients carried pathogenic DPYD variants causing DPD deficiency, including a novel intragenic deletion. The high prevalence of exon 4 deletion among Finnish patients emphasizes the importance of full-scale DPYD gene analysis and the use of genotype preemptive screening to detect DPD-deficient patients before fluoropyrimidine therapy.
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2021)
Article
Genetics & Heredity
Nina Lenherr, John Christodoulou, John Duley, Doreen Dobritzsch, Lynette Fairbanks, Alexandre N. Datta, Isabel Filges, Nicolas Guertler, Jeroen Roelofsen, Andre B. P. van Kuilenburg, Claudia Kemper, Erin E. West, Gabor Szinnai, Martina Huemer
Summary: Arts syndrome is caused by mutations in the PRPS1 gene, leading to symptoms such as hearing loss, optic atrophy, muscular hypotonia, developmental impairment, and early respiratory infections. Treatment with S-adenosylmethionine (SAM) and nicotinamide riboside has shown improvements in clinical phenotype and T-cell survival and function.
MOLECULAR GENETICS AND METABOLISM REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Linghe Wu, Linde Woudstra, Tariq A. Dam, Tjeerd Germans, Albert C. van Rossum, Hans W. M. Niessen, Paul A. J. Krijnen
Summary: The study found that viral myocarditis induces transient changes in myocardial electrical conduction that are strongly related to cardiac cellular inflammation, particularly infiltration of lymphocytes, macrophages, and neutrophils. These changes did not significantly correlate with lesion size and fibrosis.
CARDIOVASCULAR PATHOLOGY
(2021)
Article
Immunology
Matthew DeBerge, Connor Lantz, Shirley Dehn, David P. Sullivan, Anja M. van der Laan, Hans W. M. Niessen, Margaret E. Flanagan, Daniel J. Brat, Matthew J. Feinstein, Sunjay Kaushal, Lisa D. Wilsbacher, Edward B. Thorp
Summary: This study reveals the significant proinflammatory roles of myeloid cell expression of HIF-1α and HIF-2α during myocardial infarction. While HIF-2α suppresses anti-inflammatory macrophage mitochondrial metabolism, HIF-1α promotes cardioprotective factor MerTK cleavage through glycolytic reprogramming. The combined loss of myeloid HIF-1α and HIF-2α leads to catastrophic consequences, including macrophage necroptosis, impaired fibrogenesis, and cardiac rupture.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Medicine, Legal
Romy du Long, Judith Fronczek, Hans W. M. Niessen, Allard C. van der Wal, Hans H. de Boer
Summary: Determining the significance of myocardial inflammation for the cause of death can be challenging. Our study reviewed the histological spectrum of myocardial inflammation between three groups of autopsy cases, defined by their reported cause of death. Only a diffuse increase of inflammatory cells could reliably discriminate between an incidental presence of inflammation or a potentially significant one. Determining the cause of death in case of myocardial inflammation requires a comprehensive approach.
FORENSIC SCIENCES RESEARCH
(2022)
Article
Medicine, Research & Experimental
Liza Rijvers, Jamie van Langelaar, Laurens Bogers, Marie-Jose Melief, Steven C. Koetzier, Katelijn M. Blok, Annet F. Wierenga-Wolf, Helga E. de Vries, Jasper Rip, Odilia Bj Corneth, Rudi W. Hendriks, Roland Grenningloh, Ursula Boschert, Joost Smolders, Marvin M. van Luijn
Summary: Recent clinical trials have shown promising results for the next-generation BTK inhibitor evobrutinib in the treatment of multiple sclerosis. These findings demonstrate a functional link between BTK activity and disease-relevant B cells and offer valuable insights into how next-generation BTK inhibitors could modulate the clinical course of patients with MS.
Article
Pharmacology & Pharmacy
Andre B. P. van Kuilenburg, Carla E. M. Hollak, Ana Travella, Melisa Jacobs, Lucas D. Gentilini, Rene Leen, Karen M. M. Ghauharali van der Vlugt, Femke S. Beers Stet, Susan M. I. Goorden, Sanne van der Veen, Marcelo Criscuolo, Mariana Papouchado
Summary: This study found that the biosimilar AGABIO had similar amino acid sequence, glycosylation, enzymatic activity, and stability to agalsidase beta. It also showed comparable effects in cultured Fabry fibroblasts and Fabry mice. These findings support the biosimilarity of AGABIO to agalsidase beta.
Article
Urology & Nephrology
Sanne J. van der Veen, Mohamed el Sayed, Carla E. M. Hollak, Marion M. Brands, C. Khya S. Snelder, S. Matthijs Boekholdt, Liffert Vogt, Susan M. I. Goorden, Andre B. P. van Kuilenburg, Mirjam Langeveld
Summary: Fabry disease is a heterogeneous X-linked lysosomal storage disease with varying manifestations in the kidneys, heart, and brain even among patients of the same sex and classification. The study investigates the association between plasma lysoGb3 levels and disease manifestations in untreated patients with Fabry disease, and finds that higher levels of lysoGb3 are associated with disease progression in various organs.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
S. N. van der Crabben, F. L. Komdeur, E. J. Nossent, R. H. Lekanne Deprez, E. A. Broekhuizen, C. van der Werf, A. M. C. Vermeer, H. W. M. Niessen, A. C. Houweling
Summary: Sudden death, especially at a young age, may be caused by underlying genetic factors such as cardiomyopathies and arrhythmia syndromes. Identifying genetic causes allows for genetic testing and cardiological surveillance in at-risk relatives. Autopsy, genetic testing, and cardiological screening play a crucial role in evaluating sudden death cases and identifying potential genetic causes.