期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 91, 期 -, 页码 108-114出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2018.10.011
关键词
Oysters; Mitogen-activated protein kinase; Interleukins; Lipopolysaccharide
资金
- National Science Foundation of China [31530069]
- AoShan Talents Cultivation Program
- Qingdao National Laboratory for Marine Science and Technology [2017ASTCP-OS13]
- Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture, Dalian High Level Talent Innovation Support Program [2015R020]
- Research Foundation for Talented Scholars in Dalian Ocean University
P38 mitogen-activated protein kinases are serine/threonine protein kinases reportedly involved in the innate immunity of vertebrates and invertebrates. In the present study, a P38 homolog (CgP38) was characterized from the Pacific oyster Crassostrea gigas. The full-length cDNA of CgP38 was of 1515 bp containing a 1101 bp open reading frame. A serine/threonine protein kinase (S_TKc) domain with a conserved Thr-Gly-Tyr motif and an ATRW substrate-binding site was found in the deduced amino acid sequence of CgP38. CgP38 shared a close evolutionary relationship with ChP38 from the Hong Kong oyster Crassostrea hongkongensis. The transcript levels of CgP38 in hemocytes increased significantly from 12 h to 48 h after lipopolysaccharide (LPS) stimulation and from 12 h to 24 h after Vibrio splendidus stimulation. The phosphorylation level of CgP38 in oyster hemocytes increased significantly at 2 h after LPS stimulation. CgP38 positively regulated the expression of interleukins, such as CgIL17-1, CgIL17-2, CgIL17-3, CgIL17-4 and CgIL17-6, and tumor necrosis factor CgTNF after LPS or V. splendidns stimulation. These results suggested that CgP38 participated in oyster immune response by regulating the expressions of inflammatory cytokines.
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