Article
Neurosciences
Abhishek Pethe, Mira Hamze, Marina Giannaki, Bernd Heimrich, Igor Medina, Anna-Maria Hartmann, Eleni Roussa
Summary: This study investigates the effects of KCC2/NBCe1 interaction on the phosphorylation pattern and function of KCC2. The results show that KCC2 and NBCe1 interact in the mouse brain and this interaction may have pathophysiological relevance.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Physiology
Alexander M. Clifford, Martin Tresguerres, Greg G. Goss, Chris M. Wood
Summary: During exposure to acidic water, zebrafish utilize a novel mechanism of Na+ uptake that relies on the outwardly directed K+ gradient as a counter-substrate for Na+ and is sensitive to tetraethylammonium (TEA).
Article
Chemistry, Multidisciplinary
Lan Xiao, Qifan Yang, Jingjing Tan, Baofu Ma, Deliang Chen
Summary: By introducing a potential Cl- binding site in NaR, we have successfully achieved non-light modulation of NaR. The engineered NdR2 shows approximately two-fold increase in Na+ pump activity in the presence of Cl- and the lifetime of intermediates can be controlled by varying the Cl- concentration. This chemical modulation provides more diverse and precise control over cellular processes, advancing the development of optogenetic tools.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Cell Biology
Yizeng Li, Xiaohan Zhou, Sean X. Sun
Summary: Cells without a stiff cell wall, such as mammalian cells, must actively regulate their volume, with water flow determining cell volume variation. Proton and bicarbonate play significant roles in cell volume regulation, and cells need redundant mechanisms to maintain homeostasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Chia-Hao Lin, Sian-Tai Liu, Yu-Chun Wang, Yi-Ling Tsou, Huei-Jyun Hu
Summary: Maintenance of an acid-base balance is crucial for vertebrates, and fishes secrete more acid in response to acidity. This study using zebrafish as a model revealed the involvement of vitamin D in acid secretion in fishes.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
Xiaodong Peng, Linling Li, Rong Lin, Xuesi Wang, Xinmeng Liu, Yukun Li, Changsheng Ma, Yanfei Ruan, Nian Liu
Summary: This study found that empagliflozin can improve Na+ and Ca2+ handling disorders induced by ouabain in a Na+-dependent manner. The research results suggest that empagliflozin exerts its therapeutic effects by inhibiting the Na+/H+ exchanger (NHE).
CARDIOVASCULAR DRUGS AND THERAPY
(2023)
Article
Medicine, Research & Experimental
Laween Uthman, Xiaoling Li, Antonius Baartscheer, Cees A. Schumacher, Patricia Baumgart, Jeroen Hermanides, Benedikt Preckel, Markus W. Hollmann, Ruben Coronel, Coert J. Zuurbier, Nina C. Weber
Summary: Inflammation leading to oxidative stress in endothelial cells is linked to heart failure development. The study suggests that TNF-alpha can activate NHE to raise [Na+](c), triggering ROS production, and EMPA can reduce inflammation-induced ROS by inhibiting NHE activity.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Editorial Material
Urology & Nephrology
Matthew A. Bailey
Summary: Salbutamol activates NaCl cotransporter in the distal convoluted tubule and combined with high salt intake can induce hypertension in mice, which can be rescued by thiazide therapy. Phosphoproteomics identified protein phosphatase 1/inhibitor 1 as a distinct regulatory node for NaCl cotransporter activation by salbutamol. Salbutamol, commonly used in respiratory medicine, may contribute to salt sensitivity and cardiovascular risk in certain patients.
KIDNEY INTERNATIONAL
(2021)
Review
Cell Biology
Andree-Anne Marcoux, Laurence E. Tremblay, Samira Slimani, Marie-Jeanne Fiola, Fabrice Mac-Way, Alexandre P. Garneau, Paul Isenring
Summary: NKCC2 is an integral membrane protein that mediates the cotranslocation of Na+, K+, and Cl(-) ions in the TALH, allowing for the reclamation of a large fraction of filtered Na+, Cl-, Ca2+, Mg-2(+), and HCO(3)(-). Malfunction of NKCC2 can lead to various inherited disorders, illustrating its physiological importance.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Critical Care Medicine
Man Li, Junqiao Lv, Zhiqiang Wang, Liping Wang, Zhixin Qin, Chen Deng, Jinming Liu, Lin Sun
Summary: HMGB1 promotes the expression of NKCC1 and NHE1 in astrocytes and endothelial cells through activation of the HMGB1-TLR4-TRIF-NF-kappa B signaling pathway, leading to cytotoxic and ionic edema following spinal cord injury. Inhibition of HMGB1 reduces the expression of NKCC1 and NHE1 and decreases spinal cord edema. These findings are important for understanding the regulation of spinal cord edema after injury.
JOURNAL OF NEUROTRAUMA
(2023)
Article
Chemistry, Physical
Ying Shi, Pengju Wang, Wenliang Li, Yan Su
Summary: A comprehensive genetic algorithm is used to search for Cl-(H2O)(1-9) and NaCl(H2O)(1-9), and various properties of these systems are investigated. It is found that the introduction of Na+ stabilizes the ionic hydrogen bonds in Cl-(H2O)(n), while the introduction of Cl- weakens the interactions between Na+ and H2O in Na+(H2O)(n). The structures of NaCl(H2O)(n) obtained from the genetic algorithm correspond to contact ion pairs, and solvent-separated ion pair structures appear when n = 7. The infrared spectra analysis shows good agreement with experimental results, and charge analysis reveals the charge transfer from Cl- to H2O in NaCl(H2O)(n).
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Xin Tan, Jinliang Pan, Yangfan Wu, Peng Xu, Luye Sun, Kui Hu, Xia Qiu, Menglei Li, Mengxi Liu, Donglin Ma, Xiaohui Qiu
Summary: In this study, the formation of stable Na3Cl nanoclusters and a Na3Cl4 monolayer was directly observed using scanning tunneling microscopy and non-contact atomic force microscopy. The stability of these structures was confirmed through density functional theory calculations. The study also found that charge transfer between the nanoclusters and the metal substrate stabilized NaCl of non-1:1 stoichiometry.
Article
Biology
Jungmi Choi, Naoki Matoba, Daiki Setoyama, Daiki Watanabe, Yuichiro Ohnishi, Ryuto Yasui, Yuichirou Kitai, Aki Oomachi, Yutaro Kotobuki, Yoichi Nishiya, Michael Paul Pieper, Hiromi Imamura, Motoko Yanagita, Masamichi Yamamoto
Summary: The SGLT2 inhibitor, Empagliflozin, increases mitochondrial ATP levels in a mouse model of diabetes, leading to cardioprotective effects. Empagliflozin, an inhibitor of sodium-glucose co-transporter 2, has been shown to reduce cardiovascular events in patients with type 2 diabetes and established cardiovascular disease. The mechanism behind these effects is still unclear.
COMMUNICATIONS BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Yoshio Takei, Masaaki Ando, Marty K. S. Wong, Takehiro Tsukada
Summary: This study investigates the molecular mechanisms of transepithelial Cl- transport in the intestine of seawater-acclimated eels, and reveals that GN stimulates Cl- secretion. The DNDS-sensitive anion exchanger (AE) and Na+-HCO3- cotransporter (NBC) play a crucial role in Cl- uptake at the basolateral side, while an unknown DPC-sensitive Cl- channel (ClC) is responsible for Cl- secretion at the mucosal side.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Article
Peripheral Vascular Disease
Richard A. Preston, David Afshartous, Evelyn Caizapanta, Barry J. Materson, Rolando Rodco, Eileen Alonso, Alberto B. Alonso
Summary: NCC activity did not significantly affect sodium sensitivity in metabolic syndrome hypertension, with 35 mmol KCl producing a rapid natriuresis approximately half that of 50 mg HCTZ and greater kaliuresis.
Article
Biology
Alex M. Zimmer, Khatereh Shir-Mohammadi, Raymond W. M. Kwong, Steve F. Perry
JOURNAL OF EXPERIMENTAL BIOLOGY
(2020)
Article
Environmental Sciences
Preeti H. Dave, Raymond W. M. Kwong
Article
Marine & Freshwater Biology
Pankaj Puar, Som Niyogi, Raymond W. M. Kwong
AQUATIC TOXICOLOGY
(2020)
Article
Environmental Sciences
Theanuga Chandrapalan, Raymond W. M. Kwong
ENVIRONMENTAL POLLUTION
(2020)
Correction
Biology
Alex M. Zimmer, Khatereh Shir-Mohammadi, Raymond W. M. Kwong, Steve F. Perry
JOURNAL OF EXPERIMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Cosima Porteus, Yusuke Kumai, Sara J. Abdallah, Hong M. Yew, Raymond W. M. Kwong, Yihang Pan, William K. Milsom, Steve F. Perry
Summary: High external ammonia exposure affects breathing in adult zebrafish, but the response may be due to oxygen chemoreception rather than ammonia sensing. Neuroepithelial cells in gill filaments exhibit a small response to NH4Cl compared to hypercapnia. The lack of colocalization of Rh proteins with NECs in larvae suggests that the entry of ammonia into NECs is not facilitated by this family of ammonia channels.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Environmental Sciences
Yao Su, Raymond W. M. Kwong, Wenli Tang, Yanan Yang, Huan Zhong
Summary: There is evidence showing that straw return may enhance the bioavailability and risks of certain metals in crops, such as Cd, Hg, and As. Mechanisms leading to increased metal mobility and bioavailability under straw return include dissolution, complexation, and methylation, which can potentially impact the physiology and absorption of metals in plants. This study highlights the importance of re-evaluating current straw return policies, especially in metal-contaminated farmlands.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Environmental Sciences
Mohammad Naderi, Raymond W. M. Kwong
ENVIRONMENT INTERNATIONAL
(2020)
Article
Environmental Sciences
Ayaat T. Hassan, Raymond W. M. Kwong
ENVIRONMENTAL POLLUTION
(2020)
Article
Environmental Sciences
Pankaj Puar, Mohammad Naderi, Som Niyogi, Raymond W. M. Kwong
Summary: The study investigated the effects of chronic exposure to environmentally relevant sub-lethal concentrations of waterborne and dietary zinc on the development of zebrafish. Results showed increased whole body zinc in all treatment groups, with the most accumulation observed in larvae exposed to elevated levels of both waterborne and dietary zinc. Exposure to high zinc levels led to decreased calcium and manganese contents, with manganese regulation appearing more sensitive than calcium to disruption by zinc exposure. Changes in ZIP transporters, particularly zip4, zip7, and zip8, were observed following zinc exposure, with different responses based on exposure routes and timing. This study highlights the sensitivity of developing zebrafish to elevated zinc levels and the importance of considering exposure routes and life stages in toxicological assessments.
ENVIRONMENTAL POLLUTION
(2021)
Review
Environmental Sciences
Mengjie Wu, Yuelu Jiang, Raymond W. M. Kwong, Satinder Kaur Brar, Huan Zhong, Rong Ji
Summary: The study found that research on marine microplastics has evolved from the initial exploration of the presence and distribution of microplastics to a focus on the bioaccumulation and ecotoxicological effects of microplastics in marine biota. However, challenges have emerged in the past decade, including the lack of standard procedures for microplastic sampling, limited detection and identification technologies, and controversy over the potential impact of microplastics on marine ecosystems and humans.
ENVIRONMENTAL POLLUTION
(2021)
Review
Biology
Theanuga Chandrapalan, Raymond W. M. Kwong
Summary: Trace metals such as iron, copper, zinc, and manganese play essential roles in various biological processes in fish, including development, energy metabolism, and immune response. Metal absorption in fish is thought to occur through specific metal ion transporters, with a complex network of regulatory mechanisms involved in maintaining metal balance. An array of factors, such as hormonal status and environmental changes, can influence metal metabolism in fish.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Neurosciences
Mohammad Naderi, Pankaj Puar, Raha JavadiEsfahani, Raymond W. M. Kwong
Summary: This study found that early-life exposure to low concentrations of BPA and BPS increased anxiety-like behavior, while exposure to higher concentrations resulted in social deficits and impaired memory. Co-exposure with an aromatase inhibitor antagonized the effects on anxiety levels and social behaviors, while exposure to an estrogen receptor antagonist restored recognition memory in zebrafish larvae.
Article
Marine & Freshwater Biology
Mahtab Zonouzi-Marand, Mohammad Naderi, Raymond W. M. Kwong
Summary: The present study investigated the effects of exposure to cadmium selenide/zinc sulfide-containing quantum dots on the neurophysiological performance of developing zebrafish. The results showed that exposure to these quantum dots led to an increase in cadmium content, decreased swimming distance but increased swimming velocity, reduced wall-hugging behavior, and decreased locomotor activity during the dark period. Furthermore, the exposure altered the mRNA levels of genes associated with dopamine signaling and oxidative stress response.
AQUATIC TOXICOLOGY
(2022)
Review
Environmental Sciences
Joshua Moyal, Preeti H. H. Dave, Mengjie Wu, Shooka Karimpour, Satinder K. K. Brar, Huan Zhong, Raymond W. M. Kwong
Summary: In this article, the impacts of biofilm formation on microplastic properties, ecotoxicity, and fate are reviewed. The environmental factors that modulate biofilm formation and the unique components of biofilm on microplastics are summarized. The influence of biofilm formation on the physical and chemical properties of microplastics and how these changes affect their pollutant adsorption capacity are discussed.
REVIEWS OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2023)