期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 228, 期 -, 页码 26-33出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2018.11.002
关键词
Fish-meal; Immune; Protein synthesis; Low salinity; Toll pathway
资金
- China Postdoctoral Science Foundation [2018 M633234]
- China Agriculture Research Systerm-48 (CRAS 48)
- Project of Marine Fishery Science and Technology of Guangdong Province [A201601C11]
- Fundamental Research Funds for the Central Universities [161gpy36]
- Natural Science Foundation of Guangdong Province [2017A030313195]
- Project of Science and Technology of Guangzhou City [201803020006]
- Project of Modern Agriculture and Marine Biological Industry Support Programs of Shenzhen City [20170428140437749]
An 8-week feeding trial was conducted to evaluate the effect of fish-meal replacement on growth performance, protein synthesis and immune response of juvenile Pacific white shrimp, Litopenaeus vannamei reared at low salinity (7 parts per thousand). Five isonitrogenous and isolipidic diets were formulated to contain graded levels (25, 20, 15, 10 and 5%) of fish-meal. High quality alternative solutions were performed, crystalline amino acids, phytase, mannan oligosaccharides and some micro-nutrients were supplemented in the low fish-meal diets. Each diet was randomly assigned to triplicate tanks, each tank with 30 shrimp (mean weight 0.3 g), the shrimp were fed 3 times a day. Weight gain and survival were decreased with the decreasing dietary fish meal levels. When dietary fish-meal decreased, the gene expression of TOR, Raptor and eIF4E2 in hepatopancreas were decreased with the decreasing fish meal levels, eIF4E2 in intestine was decreased while 4E-BP was increased with the decreasing fish meal levels. The mRNA level of SOD in hepatopancreas decreased, and the expression of GPx and CAT increased with the decreasing FM levels. The Toll pathway was affected by dietary FM levels, the expression of Toll2, TNFSF, MyD88, Rho and p38 in intestine were increased with the decreasing FM levels. The results indicated that at low salinity condition, fish meal level lower than 15% would inhibit the protein synthesis and harm to the health of shrimp.
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