Repeatability of wide-field choroidal thickness measurements using enhanced-depth imaging optical coherence tomography

Background To examine the repeatability of choroidal thickness measurements across a 55 degrees field, in a sample of healthy young adults using wide-field enhanced-depth imaging optical coherence tomography. Methods High-resolution wide-field volumetric enhanced-depth imaging scans were obtained from the right eye of 27 adults (mean age 27 +/- 5 years) during two sessions, separated by 19 +/- 15 days, using the follow-up feature of the Spectralis instrument, while controlling for confounding factors known to influence choroidal thickness. Semi-automatic segmentation of the choroidal boundaries was corrected by a single masked observer. This process was repeated on images from the first session of 12 randomly selected subjects, allowing the intersession (n = 27) and intraobserver (n = 12) co-efficients of repeatability for regional measures of choroidal thickness to be calculated. Results The observer-related variability in choroidal thickness was highest at the fovea (intraobserver co-efficient of repeatability [95% confidence interval], 13 [7-19] mu m, p < 0.001), then reduced gradually toward the perifovea (2 [1-4] mu m, p < 0.001), and plateaued in the near-peripheral (2 [1-3] mu m) and peripheral (4 [2-6] mu m) regions. The intersession variability improved significantly from the fovea (intersession co-efficient of repeatability [95% confidence interval], 27 [16-38] mu m, p < 0.01) and parafovea (25 [15-36] mu m, p < 0.02) toward the periphery (16 [10-23] mu m). Conclusion Wide-field choroidal thickness measurements using enhanced-depth imaging optical coherence tomography are highly repeatable in the macular region, and improve in precision in more peripheral regions in healthy young adults. A change of up to 38 and 28 mu m is required to distinguish true clinical change from measurement variability in individual measurements of macular and extra-macular choroidal thickness, respectively.