期刊
CELLULAR & MOLECULAR IMMUNOLOGY
卷 16, 期 11, 页码 851-867出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41423-019-0209-1
关键词
Apoptotic cell clearance; EMT; Metastasis; Exosomal PTEN; PPAR gamma ligands
类别
资金
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT, Future Planning [2015R1A2A1A15053112, 2017R1A2B2004864, 2010-0027945]
- National Research Foundation of Korea [2015R1A2A1A15053112, 2017R1A2B2004864] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Apoptotic cell clearance by phagocytes is essential in tissue homeostasis. We demonstrated that conditioned medium (CM) from macrophages exposed to apoptotic cancer cells inhibits the TGF beta 1-induced epithelial-mesenchymal transition (EMT), migration, and invasion of cancer cells. Apoptotic 344SQ (ApoSQ) cell-induced PPAR gamma activity in macrophages increased the levels of PTEN, which was secreted in exosomes. Exosomal PTEN was taken up by recipient lung cancer cells. ApoSQ-exposed CM from PTEN knockdown cells failed to enhance PTEN in 344SQ cells, restore cellular polarity, or exert anti-EMT and anti-invasive effects. The CM that was deficient in PPAR gamma ligands, including 15-HETE, lipoxin A4, and 15d-PGJ2, could not reverse the suppression of PPAR gamma activity or the PTEN increase in 344SQ cells and consequently failed to prevent the EMT process. Moreover, a single injection of ApoSQ cells inhibited lung metastasis in syngeneic immunocompetent mice with enhanced PPAR gamma/PTEN signaling both in tumorassociated macrophages and in tumor cells. PPAR gamma antagonist GW9662 reversed the signaling by PPAR gamma/PTEN; the reduction in EMT-activating transcription factors, such as Snai1 and Zeb1; and the antimetastatic effect of the ApoSQ injection. Thus, the injection of apoptotic lung cancer cells may offer a new strategy for the prevention of lung metastasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据