期刊
BIOLOGICAL CHEMISTRY
卷 396, 期 3, 页码 235-244出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2014-0241
关键词
adipocyte differentiation; fat metabolism; MEF2s; microRNA
资金
- Major National Scientific Research Projects [2015CB943102]
- National Nature Science Foundation of China [31172185]
MicroRNAs are small non-coding RNAs that partially bind to the 3' untranslated (3'UTR) regions of target genes in animals and regulate protein production of the target transcripts. MiR-103 has been confirmed to play a critical role in lipid metabolism, however, the target genes and signaling pathway regulated by miR-103 is still unclear. In our experiment, we observed a positive function of miR-103 on the adipogenic differentiation of 3T3-L1 pre-adipocyte. Furthermore, we proved that this function of miR-103 worked through activating AKT/mTOR signal pathway and impairing target gene MEF2D. By inhibiting and over-expressing the MEF2D gene, we found that MEF2D had a negative role in regulating adipocyte key genes, and this function of MEF2D could be impaired by miR-103. In conclusion, we found that miR-103 can promote 3T3-L1 cells differentiation by targeting MEF2D and activating AKT/mTOR signal pathway. These results will shed a light on further study of microRNAs.
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