Article
Energy & Fuels
M. Faried, M. Samer, M. A. Moselhy, R. S. Yousef, A. S. Ali, R. H. Ahmed, D. A. Marrez, A. El-Hussein, E. M. Abdelsalam
Summary: This study investigates the effects of different light sources on the growth and lipid accumulation of Chlorella sorokiniana. The results show that irradiating the microalgae with He-Ne red laser increases the biodiesel yield.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Environmental Sciences
Mohamed A. Embaby, El-sayed A. Haggag, Ahemd S. El-Sheikh, Diaa A. Marrez
Summary: The biosorption of uranium by non-living biomass of Chlorella sorokiniana was studied. The optimal pH for uranium biosorption was 2.5 and the equilibrium time was 90 min. The kinetics followed a pseudo-second-order rate equation and the biosorption process fit the Langmuir isotherm model well. The morphology of the algal biomass and functional groups on the algal surface were investigated. Thermodynamic simulations indicated that the uranium biosorption process was exothermic and spontaneous at lower temperatures.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Antonio Leon-Vaz, Luis C. Romero, Cecilia Gotor, Rosa Leon, Javier Vigara
Summary: This study investigated the effect of cadmium on the microalga Chlorella sorokiniana through a proteomic approach, revealing adjustments in various metabolic pathways and an increase in pathways such as photorespiration and antioxidant enzymes. Additionally, it was found that Chlorella sorokiniana exhibits high tolerance to cadmium exposure, suggesting its potential use in bioremediation processes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Tingting Li, Na Pang, Lian He, Yuan Xu, Xinyu Fu, Yinjie Tang, Yair Shachar-Hill, Shulin Chen
Summary: The microalga Chlorella sorokiniana adjusts metabolic flux and biomass synthesis to adapt to light conditions. Under light conditions, the microalga reduces fluxes in glucose catabolism, the tricarboxylic acid cycle, and anaplerotic reaction, while activating the RuBisCO reaction.
Article
Plant Sciences
Snezana Vojvodic, Milena Dimitrijevic, Milan Zizic, Tanja Ducic, Giuliana Aquilanti, Marina Stanic, Bernd Zechmann, Jelena Danilovic Lukovic, Dalibor Stankovic, Milos Opacic, Arian Morina, Jon K. Pittman, Ivan Spasojevic
Summary: This paper reports a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Agricultural Engineering
Kejing Song, Zhenzhen Zhou, Yaxin Huang, Lin Chen, Wei Cong
Summary: In this study, a thermotolerant strain of Chlorella sorokiniana, TR42, was generated with excellent cell growth and biomass production at high temperatures. The investigation of TR42's high-temperature resistant mechanism provides potential targets for enhancing microalgae's tolerance to high temperatures.
BIORESOURCE TECHNOLOGY
(2023)
Article
Energy & Fuels
B. Namitha, Asha Sathish, P. Senthil Kumar, K. Nithya, Shyam Sundar
Summary: This study compared the performance of Monoraphidium sp. and Chlorella sorokiniana in the energy conversion process, finding that Chlorella sorokiniana has higher yield and more favorable fatty acid profile. The use of biodiesel showed high lipid recovery and lower CO and HC emissions, but slightly higher nitrogen oxides and carbon dioxide emissions.
Article
Biotechnology & Applied Microbiology
Manisha Nanda, Krishna Kumar Jaiswal, Vinod Kumar, Mikhail S. Vlaskin, Pankaj Gautam, Vivekanand Bahuguna, P. K. Chauhan
Summary: Green oleaginous microalgae are promising candidates for the integration of heavy metal bioremediation with biofuel production. In this study, Chlorella sorokiniana was used for lead mitigation and biodiesel production, showing high tolerance to lead exposure and altered lipid profiles under stress conditions. This research provides valuable insights into the potential applications of oleaginous microalgae in environmental remediation and sustainable biofuel production.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Agricultural Engineering
Youping Xie, Zhen Zhang, Ruijuan Ma, Xinyu Liu, Miao Miao, Shih-Hsin Ho, Jianfeng Chen, Yoong Kit Leong, Jo-Shu Chang
Summary: This study focuses on the heterotrophic cultivation of Chlorella sorokiniana FZU60 to improve the production efficiency of lutein. Through flask cultivation and fermenter experiments, it was found that the feeding strategy 3F achieved the highest lutein production and productivity, surpassing reported values.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Hong Fang, Junjie Liu, Ruijuan Ma, Yiping Zou, Shih-Hsin Ho, Jianfeng Chen, Youping Xie
Summary: This study isolated and identified the coding genes of CsLCYB and CsLCYE in the lutein-enriched microalga Chlorella sorokiniana FZU60. Bioinformatics analysis revealed the key structural domains of CsLCYB and CsLCYE. CsLCYB efficiently beta-cyclized lycopene to produce beta-carotene, while CsLCYE exhibited strong epsilon-monocyclase activity for delta-carotene production and weak -bicyclic activity for -carotene production. Co-expression of CsLCYB and CsLCYE resulted in the major production of alpha-carotene, suggesting the potential for high-level lutein production in C. sorokiniana FZU60.
Article
Ecology
Oskar A. Palacios, Jose C. Espinoza-Hicks, Alejandro A. Camacho-Davila, Blanca R. Lopez, Luz E. de-Bashan
Summary: This study characterized the metabolites exuded during the mutualistic interaction between the microalga Chlorella sorokiniana and the microalgae growth-promoting bacteria Azospirillum brasilense. The results showed that different strains of C. sorokiniana exuded different carbon compounds, and the type of carbon source was more important for sustaining the growth of the partner bacteria.
Article
Biochemistry & Molecular Biology
Agata Piasecka, Andrea Baier
Summary: This study investigated the impact of three trophic modes on the growth parameters and biochemical composition of Chlorella species. The results showed that mixotrophic cultivation using an agro-industrial by-product stimulated the growth of all species, yielding high amounts of protein-rich biomass. The study also provided insights into the proteome of Chlorella cells cultured under molasses supplementation conditions.
Article
Agricultural Engineering
Mohamad Padri, Nittaya Boontian, Neung Teaumroong, Pongdet Piromyou, Chatlada Piasai
Summary: This study shows that the co-culture of a microalga and bacterium can effectively treat wastewater and enhance biodiesel production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Marine & Freshwater Biology
Antonio Leon-Vaz, Rosa Leon, Inmaculada Giraldez, Jose Maria Vega, Javier Vigara
Summary: The microalga Chlorella sorokiniana was tested for bioremediation of heavy metals pollution, showing significant inhibition on growth and ammonium consumption at high concentrations of Cu2+ and As (III). It exhibited outstanding Cd accumulation ability, with antioxidant enzymes and enzymes involved in glutamate synthesis showing different levels of upregulation in response to Cd and As stress, except for the downregulation of GS expression.
AQUATIC TOXICOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Pingkang Qian, Kun Gao, Zheng Gao, Chunye Xue, Xiaoli Hu, Qiaoqiao Liu, Jingwei Dong, Xiangyuan Deng
Summary: The study showed that myo-inositol (MI) can promote the growth and regulate the biochemical compositions of Chlorella sorokiniana, while having a negative impact on photosynthetic activity. Adjusting the MI concentration can affect the content of chlorophyll a and enhance algal photosynthesis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Agricultural Engineering
Ranjna Sirohi, Ashutosh Kumar Pandey, Panneerselvam Ranganathan, Shikhangi Singh, Aswathy Udayan, Mukesh Kumar Awasthi, Anh Tuan Hoang, Chaitanya Reddy Chilakamarry, Sang Hyoun Kim, Sang Jun Sim
Summary: There has been increasing attention on the use of photobioreactors for microalgae cultivation. Understanding the state-of-the-art designs, advantages, and limitations is crucial for classifying and applying different photobioreactors effectively.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Aswathy Udayan, Ranjna Sirohi, Nidhin Sreekumar, Byoung-In Sang, Sang Jun Sim
Summary: Microalgae are unicellular photosynthetic organisms that can produce high-value metabolites, making them potential candidates for nutraceutical and industrial products. Research on cost-effective cultivation systems and harvesting methods is crucial for increasing biomass yield and reducing downstream processing costs to fully utilize the potential of microalgae in industry.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Ayon Tarafdar, Ranjna Sirohi, Palanisamy Athiyaman Balakumaran, R. Reshmy, Aravind Madhavan, Raveendran Sindhu, Parameswaran Binod, Yogesh Kumar, Deepak Kumar, Sang Jun Sim
Summary: Bisphenol A (BPA) is a toxic endocrine disrupting chemical with immunosuppressive activity, disrupting the endogenous hormones and requiring proper detection and remediation methods. More research is needed on integrated remediation technologies and the impact of food processing operations on BPA.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Inhwa Song, Sunbin Kim, Jongrae Kim, Hyeonjun Oh, Junhwan Jang, Su Jin Jeong, Kwangryul Baek, Weon-Sun Shin, Sang Jun Sim, EonSeon Jin
Summary: This study successfully generated Chlamydomonas reinhardtii mutants that are rich in both carotenoids and fatty acids by editing the AGP gene and zep mutant. These mutants can be used to produce microalgal oil enriched with macular pigments.
MICROBIAL CELL FACTORIES
(2022)
Review
Plant Sciences
Sabeela Beevi Ummalyma, Ranjna Sirohi, Aswathy Udayan, Pooja Yadav, Abhay Raj, Sang Jun Sim, Ashok Pandey
Summary: Microalgae are cell factories that can be used to produce renewable energy, food, feed, pharmaceuticals, and nutraceuticals. The challenges of mass cultivation and biomass recovery limit the industrial application of microalgae. Cultivating microalgae in wastewater has been encouraged for sustainable bioeconomy. Wastewater from the food industry provides a less-toxic growth medium for low-cost microalgal biomass production. Microalgae are potential biocatalysts for wastewater bioremediation and offer a universal alternative for resource recovery from wastewater.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Agricultural Engineering
Jaemin Joun, Ranjna Sirohi, Sang Jun Sim
Summary: This study compared the culture method using sodium acetate and glucose as organic carbon sources with autotrophy in Haematococcus pluvialis. In the 12-day culture, mixotrophy with sodium acetate and glucose increased biomass by 40.4% and 77.1% respectively compared to autotrophy, but the mechanisms were different. Mixotrophy with glucose increased biomass by directly supplying substrate and ATP to the TCA cycle while inhibiting photosynthesis. Gene expressions related to glycolysis and carbon fixation pathway were confirmed in autotrophy and mixotrophy with glucose and acetate. The proposed metabolism in mixotrophy with acetate and glucose was analyzed through autotrophic and heterotrophic metabolism.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Young Joon Sung, Byung Sun Yu, Ha Eun Yang, Dong Hoon Kim, Ju Yeon Lee, Sang Jun Sim
Summary: This study provides an in-depth analysis of microalgae-based biohydrogen production, focusing on large-scale cultivation systems and efficient downstream processes. Strategies to reduce carbon emissions and improve productivity simultaneously are discussed by considering each production process. This research has significant implications for achieving environmental and economic sustainability.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Jeong Seop Lee, Young Joon Sung, Dong Hun Kim, Ju Yeon Lee, Sang Jun Sim
Summary: A hybrid system for fabricating an easy-to-scale-up photosynthetic bioreactor (PBR) was developed, and the design of the baffle and the conditions of the gas sparger were optimized to improve biomass productivity. The newly developed PBR showed improved PHB production feasibility.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Byung Sun Yu, Ha Eun Yang, Ranjna Sirohi, Sang Jun Sim
Summary: This study investigates the effects of microalgae-based biomineralization on biomass productivity and CO2 fixation rate. The results show that this method significantly improves biomass production and CO2 fixation rate by allowing cells to conduct photosynthesis more efficiently.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Ha Eun Yang, Byung Sun Yu, Sang Jun Sim
Summary: The aim of this study was to increase the productivity of biomass with high astaxanthin content by developing a mutant strain of Haematococcus pluvialis with strong environmental tolerance. The mutant M5 strain, selected through gamma irradiation, showed improved biomass and astaxanthin production under high-salinity and high-light intensity conditions. M5 demonstrated an increase in biomass and astaxanthin productivity by 86.70% and 66.15% respectively, compared to untreated cells.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
So Young Lee, Jeong Seop Lee, Sang Jun Sim
Summary: Wastewater treatment plants emit a large amount of greenhouse gases, but mixotrophic microalgae cultivation using wastewater has been proposed as a solution for CO2 mitigation and wastewater treatment. This study used waste soy sauce as a medium for Chlorella sorokiniana UTEX 2714 cultivation and found that using a 20% (v/v) 10-fold diluted soy sauce medium increased biomass and fatty acid methyl ester (FAME) productivity by 1.93 and 1.76 times, respectively. High biomass productivity was achieved under high-intensity light with a high soy sauce content, although it inhibited cell growth in photoautotrophic environments. Furthermore, the combination of industrial flue gas treatment with wastewater led to a 42% improvement in biomass production. These results suggest that mixotrophic microalgal cultivation has the potential to address climate change and water pollution while producing valuable products and contributing to a sustainable society.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biophysics
Sojin Song, Jong Uk Lee, Myeong Jin Jeon, Soohyun Kim, Chan-Nyoung Lee, Sang Jun Sim
Summary: Alzheimer's disease is a neurodegenerative condition that is challenging to diagnose early due to the lack of robust biomarkers and limited access to neuroimaging and cerebrospinal fluid tests. Exosomes in body fluids are being explored as diagnostic biomarkers that reflect neuropathological features. A programmable curved plasmonic nanoarchitecture-based biosensor has been developed to accurately diagnose Alzheimer's disease.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Plant Sciences
Jaemin Joun, Ranjna Sirohi, Byung Sun Yu, Ha Eun Yang, Sang Jun Sim
Summary: Microalgae cultivation using wastewater is an effective method for wastewater treatment and carbon footprint reduction. Wastewater provides essential nutrients for microalgae growth, while greenhouse gas emissions can be mitigated through Carbon Capture, Utilization, and Storage technology. This review comprehensively discusses the application of microalgae in capturing carbon and treating wastewater, highlighting the potential for improving productivity, reducing costs, and promoting environmental sustainability.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Ranjna Sirohi, V. Vivekanand, Ashutosh Kumar Pandey, Ayon Tarafdar, Mukesh Kumar Awasthi, Amita Shakya, Sang Hyoun Kim, Sang Jun Sim, Hoang A. Tuan, Ashok Pandey
Summary: The transition from fossil fuels to non-conventional sources is necessary for addressing the global energy crisis and environmental issues. Organic waste from various industries can be utilized to produce gaseous fuels through different biochemical strategies. However, current biogas and biohydrogen production practices are inefficient and require interventions to improve biofuel yield. Adding biochar has been shown to enhance gaseous fuel production by adsorbing inhibitors and activating gas-producing microorganisms. This review explores the effect of biochar on gaseous biofuel production and highlights the need for a circular bioeconomy approach.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Chemistry, Multidisciplinary
Sang Ki Kim, Jong Uk Lee, Myeong Jin Jeon, Soo-Kyung Kim, Sang-Hyun Hwang, Min Eui Hong, Sang Jun Sim
Summary: A colorimetric-fluorescent dual-mode lateral flow immunoassay sensor incorporating metal-enhanced fluorescence (MEF) was developed for the rapid and sensitive serological detection of SARS-CoV-2 IgG.