期刊
BIOSENSORS & BIOELECTRONICS
卷 126, 期 -, 页码 664-671出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.11.034
关键词
Antibiotic; Aptamer; Field-effect transistor; Microfluidic chip; Debye length
类别
资金
- National Natural Science Foundation of China [21707102]
- 1000 Talents Plan of China
Antibiotic residue, as emerging pollution resulting from antibiotic abuse, poses a serious threat on ecosystem and human health. Conventional methods for antibiotic detection, e.g., liquid/gas chromatography, are based on complicated instruments and time-consuming; therefore, efforts have been made to realize in situ and real-time monitoring of antibiotics. Here, a miniaturized and integratable electronic antibiotic sensor based on field-effect transistor (FET) is reported. The reduced graphene oxide (rGO) nanosheet is used as the channel material and the aptamer RNA for tobramycin is modified onto rGO as the probe. A novel sensor design with 6-mercapto-lhexanol (MCH)/1-pyrenebutanol (PBA) blocking layer (BL) for structure optimization is applied to enhance the sensor reliability and specificity. This rGO/aptamer/BL sensor shows an ultra -sensitivity to tobramycin with a lower detection limit of 0.3 nM and a quick response within 5 s, as well as a high specificity over other antibiotics such as kanamycin, streptomycin, ciprofloxacin, and tetracycline. The sensing mechanism based on the deformation of the charged aptamer probe is proposed via an in-depth analysis of the interactions between aptamer, tobramycin and rGO. In addition, sensing test performed under controlled microfluidic flow conditions demonstrates a great potential of the sensors in practical applications.
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