Graphene nanogap electrodes in electrical biosensing

Graphene nanogap electrodes are reported here for the first time in an electrical biosensor for the detection of biomolecular interactions. Streptavidin-biotin was chosen as a model system for evaluating the sensor's performance. High-affinity interactions of streptavidin-gold nanoparticles (strep-AuNPs) to the biotin-functionalized nanogap localizes AuNPs, thereby bridging the gap and resulting in changes in device conductance. Biosensing performance was optimized by varying the gap size, AuNP diameter, and streptavidin coverage on AuNPs. The sensitivity and limit of detection (LOD) of streptavidin detection with the optimized parameters were determined to be 0.3 mu A/nM and 0.25 pM, respectively. The proposed platform suggests high potential as a portable point-of-use biosensor for the detection of other affinity-based biomolecular interactions, such as antigen-antibody, nucleic acid, or chemo-selective interactions.