期刊
BIORESOURCE TECHNOLOGY
卷 273, 期 -, 页码 538-544出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2018.11.046
关键词
Polyhydroxyalkanoates; Pseudomonas putida KT2440; Benzoate; Nitrogen; Kinetic modeling
资金
- U.S. Department of Energy (DOE), the Office of Energy Efficiency & Renewable Energy (EERE) Awards [DE-EE0007104, DE-EE0006112]
- Bioproducts, Science and Engineering Laboratory, Department of Biological Systems Engineering at Washington State University
- U.S. Department of Energy's Office of Biological and Environmental Research
- CSC
Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. In this study, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC-MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. These results provide a promising strategy for optimization of lignin to PHA yields.
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