期刊
BIOELECTROCHEMISTRY
卷 125, 期 -, 页码 90-96出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2018.09.006
关键词
Poly(carboxybetaine methacrylate); Polyaniline; Antifouling; Carcinoembryonic antigen; Immunosensor
资金
- National Natural Science Foundation of China [21705088]
- Shandong Science and Technology Program [J14LB14]
- Natural Science Foundation of Shandong Province of China [ZR2016BM05, ZR2017MEE016, ZR2017BD038]
Biocompatible materials, such as zwitterionic poly(carboxybetaine methacrylate) (polyCBMA), are of extraordinary importance in growth of bioelectronics and biosensors, because they not only greatly suppress nonspecific protein adsorption, but also have rich functional groups to facilitate the fixation of biological molecules. A novel nanocomposite was synthesized herein through modification polyCBMA onto conducting polymer polyaniline (PANI) nanowire surface. The prepared polyCBMA/PANI composite, integrating the good conductivity of PANI nanowires with the excellent antifouling capability of polyCBMA, provided a wonderful matrix for the growth of ultrasensitive and low fouling biosensor. Carcinoembryonic antigen (CEA), an important biomarker for a variety of cancers, was utilized as a model test. Furthermore its antibody was fixed onto polyCBMA/PANI for the preparation of CEA biosensor. DPV was applied as a sensing principle with information at which peak potential and current the signals were recorded. The peak currents were in inverse proportion to the logarithm of CEA concentration in the range from 1.0 x 10(-14) g mL(-1) to 1.0 x 10(-10) g mL(-1), with a detect limit of 3.05 fg mL(-1). Furthermore, this low fouling, label-free biosensor has been utilized for assaying in undiluted human serum samples with resisting serious nonspecific protein adsorption, demonstrating its feasible potential application in clinical analysis of CEA. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据