Article
Biochemistry & Molecular Biology
Jiahui Ding, Paulomi Aldo, Cai M. Roberts, Paul Stabach, Hong Liu, Yuan You, Xuemin Qiu, Jiwon Jeong, Anthony Maxwell, Brett Lindenbach, Demetrios Braddock, Aihua Liao, Gil Mor
Summary: The study identified ISG20 as a key component in controlling Zika virus infection and successfully developed a recombinant ISG20-Fc protein that could potentially serve as an antiviral treatment option for high-risk populations, especially pregnant women.
Article
Virology
Xiaoyong Chen, Dage Sun, Sujie Dong, Huanjie Zhai, Ning Kong, Hao Zheng, Wu Tong, Guoxin Li, Tongling Shan, Guangzhi Tong
Summary: ISG20 plays a role in suppressing pseudorabies virus replication by enhancing IFN signaling, and its expression is upregulated following PRV infection. The PRV protein UL24 can suppress the transcription of ISG20, thus antagonizing its antiviral effect. These findings provide insights into the interplay between host and PRV.
Article
Pharmacology & Pharmacy
Youichi Suzuki
Summary: Chikungunya virus (CHIKV) is a enveloped RNA virus that causes a disease called Chikungunya fever (CHIKF), which is transmitted by infected Aedes mosquitos. Although CHIKF was previously only considered endemic in limited regions of Africa and Asia, the recent global reemergence of CHIKV has increased awareness of this infectious disease, making it a growing public health threat. Currently, there are no specific drugs or licensed vaccines available to treat CHIKV infection. This review focuses on the molecular mechanisms of how the interferon (IFN) response suppresses CHIKV, particularly the role of interferon-stimulated genes (ISGs) in restricting CHIKV replication.
ANTIVIRAL RESEARCH
(2023)
Article
Microbiology
Jinchao Xing, Chen Hu, Siqi Che, Yixin Lan, Lihong Huang, Lele Liu, Youqin Yin, Huanan Li, Ming Liao, Wenbao Qi
Summary: The UAF1 protein is involved in the replication of Japanese encephalitis virus (JEV) and regulates autophagy, which promotes JEV replication. This study provides new insights into the functional annotation of UAF1 and its antiviral effect against JEV.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Jennifer Deborah Wuerth, Friedemann Weber
Summary: Sandfly fever Sicilian virus (SFSV) in the Phlebovirus genus dampens but doesn't completely abolish type I and type III IFN induction through its NSs protein, allowing for substantial ISG expression in infected cells.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Federico Marziali, Mathilde Delpeuch, Anuj Kumar, Romain Appourchaux, Jeremy Dufloo, Kevin Tartour, Lucie Etienne, Andrea Cimarelli
Summary: IFITMs are a family of interferon-inducible proteins that inhibit a broad range of viruses by interfering with viral-to-cellular membrane fusion. By comparing 21 IFITMs from different animal species for their ability to inhibit HIV-1, functional diversity was identified, not driven by known domains and only partly explained through protein stability. Chimeras between active and inactive IFITMs suggest that the cross talk between individual IFITM domains is important for optimal antiviral activity.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Sambamurthy Chandrasekaran, Joshua A. Kochanowsky, Emily F. Merritt, Joseph S. Lagas, Ayesha Swannigan, Anita A. Koshy
Summary: The study shows that neurons are capable of clearing intracellular Toxoplasma gondii parasites through immune-regulated GTPases. Both murine and human neurons can mount cytokine-dependent defense mechanisms against T. gondii infection.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jacob Borold, Davide Eletto, Idoia Busnadiego, Nina K. Mair, Eva Moritz, Samira Schiefer, Nora Schmidt, Philipp P. Petric, W. Wei-Lynn Wong, Martin Schwemmle, Benjamin G. Hale
Summary: The study identified BRD9 as a critical factor for IFN-induced ISG expression, and genetic knockout or degradation of BRD9 can limit ISG expression and attenuate the antiviral activity of IFN. BRD9 acts at the transcriptional level through interactions with factors like STAT2, possibly serving as an important regulatory factor for inhibiting ISG expression.
Article
Biochemistry & Molecular Biology
Nicole E. Bowen, Joshua Temple, Caitlin Shepard, Adrian Oo, Fidel Arizaga, Priya Kapoor-Vazirani, Mirjana Persaud, Corey H. Yu, Dong-Hyun Kim, Raymond F. Schinazi, Dmitri N. Ivanov, Felipe Diaz-Griffero, David S. Yu, Yong Xiong, Baek Kim
Summary: Mutations in SAMHD1, specifically the R366C/H mutants found in colon cancer and leukemia, lead to loss of dNTPase activity which may contribute to the elevated dNTP levels commonly observed in cancer cells. These mutants retain other functions but fail to reduce dNTP levels and restrict HIV-1 replication.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Virology
Xiu-Qing Li, Lei Zeng, Dong-Ge Liang, Yan-Li Qi, Guo-Yu Yang, Kai Zhong, Bei-Bei Chu, Jiang Wang
Summary: PRV belongs to the alphaherpesvirus subfamily and can establish and maintain a lifelong latent infection in pigs. The intermittent active cycle of PRV poses great challenges to the prevention and control of PRV disease and causes serious economic losses to the pig breeding industry.
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Bruno A. Rodriguez-Rodriguez, Grace O. Ciabattoni, Ralf Duerr, Ana M. Valero-Jimenez, Stephen T. Yeung, Keaton M. Crosse, Austin R. Schinlever, Lucie Bernard-Raichon, Joaquin Rodriguez Galvan, Marisa E. McGrath, Sanjay Vashee, Yong Xue, Cynthia A. Loomis, Kamal M. Khanna, Ken Cadwell, Ludovic Desvignes, Matthew B. Frieman, Mila B. Ortigoza, Meike Dittmann
Summary: The authors develop a neonatal mouse model for studying SARS-CoV-2 transmission and show that deletion of ORF8 leads to a shift in viral replication and delayed transmission. Small animal models have posed challenges in studying SARS-CoV-2 transmission, with most researchers using golden hamsters or ferrets. However, mice offer advantages such as lower cost, wider availability, and existing reagents and genetic tools. The neonatal mouse model established here allows for transmission of clinical SARS-CoV-2 isolates and can be used to study viral and host determinants.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Hong-My Nguyen, Shreyas Gaikwad, Mariam Oladejo, Manas Yogendra Agrawal, Sanjay K. Srivastava, Laurence M. Wood
Summary: Type 1 interferons are central in regulating the immune system's response and induce interferon-stimulated genes (ISGs). Among them, ISG15 has been extensively studied for its role in cancer as both a promoter and inhibitor of tumor growth. This review provides an update on recent observations and findings related to ISG15 in cancer, including its molecular mechanisms and therapeutic applications.
Article
Multidisciplinary Sciences
Zhongshun Liu, Congwei Jiang, Zhangmengxue Lei, Sihan Dong, Linlin Kuang, Chenxu Huang, Ying Gao, Mu Liu, Hui Xiao, Patrick Legembre, Jae U. Jung, Huaping Liang, Xiaozhen Liang
Summary: Type I interferons (IFNs) are the first line of defense against invading pathogens. This study identified a previously unknown protein, PINLYP, that interacts with TBK1 to induce the production of type I IFN. Loss of PINLYP impaired the activation of IRF3 and the production of IFN induced by various viruses and Toll-like receptor ligands. Mice lacking PINLYP were more susceptible to lethal virus infection, highlighting the importance of PINLYP in the host defense against viral infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Yanxia Huang, Yu Su, Li Shen, Zhiting Huo, Cancan Chen, Tao Sun, Xu Tian, Ning Li, Chao Yang
Summary: This study reveals that host lncRNA ZAP-IT1 induced by type I IFN signaling has a strong restriction on ZIKV infection, as well as other viral infections, which may benefit the development of antiviral therapeutics.
Article
Virology
Connor G. G. Bamford, Elihu Aranday-Cortes, Ricardo Sanchez-Velazquez, Catrina Mullan, Alain Kohl, Arvind H. Patel, Sam J. Wilson, John McLauchlan
Summary: The cellular gene ARHGEF3 has been found to inhibit the replication of HCV and other Flaviviridae viruses, and this activity is conserved between humans and rhesus macaques.
Review
Biochemistry & Molecular Biology
Tandrila Das, Jacob S. Yount, Howard C. Hang
Summary: S-palmitoylation is a reversible posttranslational lipid modification of proteins, regulating protein activity, stability, trafficking, and interactions. Recent studies have identified many S-palmitoylated immunity-associated proteins in immune cells, which can drive therapeutic advances in modulating immune responses.
Article
Immunology
Adrienne M. Antonson, Adam D. Kenney, Helen J. Chen, Kara N. Corps, Jacob S. Yount, Tamar L. Gur
Summary: Research found that mild or moderately pathogenic IAV infection during pregnancy does not induce inflammation in the developing fetal brain, and did not observe the hallmarks of mimetic-induced MIA, highlighting the importance of using live pathogen infection models for MIA study.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Elena Kudryashova, Ashley Zani, Geraldine Vilmen, Amit Sharma, Wuyuan Lu, Jacob S. Yount, Dmitri S. Kudryashov
Summary: This study found that human defensin HNP1 can bind to the spike protein of SARS-CoV-2 and has strong antiviral activity. Defensins can interfere with spike protein-mediated membrane fusion, viral infection, and interaction with the ACE2 receptor. The activity of defensins is influenced by serum, but they can still effectively inactivate the virus at high concentrations. These findings suggest that defensins may be valuable tools in developing strategies to prevent SARS-CoV-2 infection.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Virology
Mijia Lu, Yuexiu Zhang, Piyush Dravid, Anzhong Li, Cong Zeng, K. C. Mahesh, Sheetal Trivedi, Himanshu Sharma, Supranee Chaiwatpongsakorn, Ashley Zani, Adam Kenney, Chuanxi Cai, Chengjin Ye, Xueya Liang, Jianming Qiu, Luis Martinez-Sobrido, Jacob S. Yount, Prosper N. Boyaka, Shan-Lu Liu, Mark E. Peeples, Amit Kapoor, Jianrong Li
Summary: The study developed a recombinant vesicular stomatitis virus (mtdVSV)-based SARS-CoV-2 vaccine candidate, which was proven to be safe and highly efficacious in mouse and hamster experiments. It induced high levels of neutralizing antibodies and showed promise in providing protection against SARS-CoV-2 infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cong Zeng, Abdul A. Waheed, Tianliang Li, Jingyou Yu, Yi-Min Zheng, Jacob S. Yount, Haitao Wen, Eric O. Freed, Shan-Lu Liu
Summary: SERINC3 and SERINC5, known as host restriction factors against HIV, were found to exhibit additional antiviral activities by enhancing the expression of genes encoding type I interferons and nuclear factor kappa B signaling. SERINC5 interacts with MAVS and TRAF6, leading to enhancement of antiviral activities and inhibition of HIV-1 and rVSV infection. These findings suggest a novel function of SERINC proteins in modulating host inflammatory signaling and antiviral responses.
Article
Infectious Diseases
Qin-Yan Chen, Hui-Hua Jia, Xue-Yan Wang, Yun-Liang Shi, Lu-Juan Zhang, Li-Ping Hu, Chao Wang, Xiang He, Tim J. Harrison, J. Brooks Jackson, Li Wu, Zhong-Liao Fang
Summary: This study investigated the evolutionary data of HBV genomes using next generation sequencing and found that mutations in HBV may revert to wild type in natural infection. Sequencing of HBV genomes revealed specific mutations in some subjects in 2007, which were absent in 2019.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Immunology
BradleyS Barrett, David H. Nguyen, Joella Xu, Kejun Guo, Shravida Shetty, Sean T. Jones, Kaylee L. Mickens, Caitlin Shepard, Axel Roers, Rayk Behrendt, Li Wu, Baek Kim, Mario L. Santiago
Summary: SAMHD1 is an antiretroviral factor in vivo that inhibits acute retrovirus infection in mice, and its immunological phenotype is influenced by sex and the presence of inflammation. SAMHD1 knockout mice showed higher viral loads and weaker immune responses compared to wild-type mice. This study highlights the importance of SAMHD1 in antiretroviral immunity and suggests that gut barrier dysfunction may affect its antiviral function.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Microbiology
Colton Martens, Lisa A. Dorn, Adam Kenney, Shyam A. Bansal, Jacob Yount, Federica A. Accornero
Summary: This study identifies BEX1 as a novel antiviral protein that plays a cardioprotective role in the heart during viral infection. It regulates cardiac immune responses to limit viral replication and its absence accelerates virus-induced heart failure and pathological remodeling, while its overexpression confers protection.
Article
Virology
Roberth Anthony Rojas Chavez, Devlin Boyt, Nathan Schwery, Changze Han, Li Wu, Hillel Haim
Summary: Infection by HIV-1 leads to the formation of antibodies that target the viral Env proteins and can render the virus inactive. The targets of these antibodies vary among infected individuals. This study suggests that antibodies against the base of the Env protein are commonly produced during infection, but their selective pressure is weak. As a result, these antibodies do not completely eliminate the sensitive forms of the virus from the population, but maintain their frequency at a low level since the beginning of the AIDS pandemic. Interestingly, the changes in Env do not occur at the sites targeted by the antibodies, but at a distinct region called the fusion peptide-proximal region (FPPR), which regulates their exposure.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Stacia Phillips, Tarun Mishra, Shaubhagya Khadka, Dana Bohan, Constanza E. Espada, Wendy Maury, Li Wu
Summary: Posttranscriptional modification of viral and cellular RNAs by N-6-methyladenosine (m(6)A) is important in regulating virus replication and cellular immune response. This study analyzed the m(6)A profile of human lung epithelial cells infected with SARS-CoV-2, and found differential methylation of mRNA and noncoding RNA. These modifications may have important functions in the cellular response to infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Genetics & Heredity
Hui-Hua Jia, Qin-Yan Chen, Zhi-Hua Jiang, Xue-Yan Wang, Wen-Jia Zhang, Xiang He, Tim J. Harrison, J. Brooks Jackson, Li Wu, Zhong-Liao Fang
Summary: This study investigated the molecular evolution of hepatitis B virus (HBV) genotype I over a 15-year period using both NGS and clone-based sequencing. Results showed the presence of novel subgenotypes and recombinant strains between genotype I1 and subgenotype C. The intrahost viral evolutionary rate was calculated to be 3.88E-4 substitutions per site per year, and the genetic diversity ranged from 0.0022 to 0.0041.
Article
Oncology
Zhi-Hua Jiang, Qin-Yan Chen, Hui-Hua Jia, Xue-Yan Wang, Lu-Juan Zhang, Xiao-Qian Huang, Tim J. Harrison, J. Brooks Jackson, Li Wu, Zhong-Liao Fang
Summary: This study investigated the association between HBV double mutations and HCC. The study suggests that the change in the values of mean intrahost quasispecies genetic distances in HCC is smaller, indicating low host immune pressure. Increasing viral loads and novel mutations may increase the risk for HCC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yingke Tang, Ryan T. Behrens, Corine St Gelais, Siqi Wu, Saravanan Vivekanandan, Ehud Razin, Pengfei Fang, Li Wu, Nathan Sherer, Karin Musier-Forsyth
Summary: Human lysyl-tRNA synthetase (LysRS) is re-localized from cytoplasm to nucleus in HIV-1 infected cells through phosphorylation. Phosphorylation promotes HIV-1 transcription, and nuclear pS207-LysRS generates Ap4A, which also activates HIV-1 transcription. Additionally, MSC-derived peptide stabilizes LysRS MSC binding and inhibits HIV-1 replication.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Stacia Phillips, Alice Baek, Sanggu Kim, Shuliang Chen, Li Wu
Summary: This study describes a method to manipulate the expression of m6A demethylases in HIV-1 producer cells, allowing for the production of HIV-1 with altered m6A levels. The RNA from purified virions is analyzed to determine the m6A levels, which are then used in downstream assays to investigate the function of m6A modification of viral RNA.
Article
Multidisciplinary Sciences
Matthew J. Mears, Michael J. Wallace, Jacob S. Yount, Lorri A. Fowler, Penny S. Jones, Peter J. Mohler, Loren E. Wold
Summary: The implementation of precautionary measures like physical isolation, social distancing, and masks has been crucial in controlling the spread of COVID-19. This paper highlights the innovative repurposing of lab materials and personnel to address the shortage of virus transport media, emphasizing the ability of standard research labs to meet pandemic-related needs. The collaboration at The Ohio State University Wexner Medical Center allowed for efficient production and distribution of VTM tubes, facilitating mass COVID-19 testing.
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)