Article
Agronomy
Wael Toukabri, Nouha Ferchichi, Meriem Barbouchi, Dorsaf Hlel, Mohamed Jadlaoui, Haithem Bahri, Ridha Mhamdi, Hatem Cheikh M'hamed, Mohamed Annabi, Darine Trabelsi
Summary: The study revealed that the Pseudomonas thivervalensis T124 strain can benefit clover growth and development under dense stand conditions by alleviating the negative effects of reduced light and exogenous ethylene, improving clover tolerance to shade.
Article
Agricultural Engineering
Ramachandran Srinivasan, Parthiban Subramanian, Srikanth Tirumani, Kodiveri Muthukaliannan Gothandam, Mohandass Ramya
Summary: The study demonstrated that expressing the ACC deaminase gene in Chlamydomonas led to improved tolerance to nitrogen deficiency and enhanced growth activity, as well as increased lipid and fatty acid content.
BIORESOURCE TECHNOLOGY
(2021)
Article
Plant Sciences
Noris J. Flores-Duarte, Julia Perez-Perez, Salvadora Navarro-Torre, Enrique Mateos-Naranjo, Susana Redondo-Gomez, Eloisa Pajuelo, Ignacio D. Rodriguez-Llorente
Summary: The study shows that Variovorax strains can promote the growth of legume crops in poor and degraded soils, improve the physiological status of plants, and enhance their response to stress and metal accumulation. The best results were observed when the three bacteria were co-inoculated.
Article
Biochemistry & Molecular Biology
Maria A. Lebedeva, Daria A. Dobychkina, Lyudmila A. Lutova, Abir U. Igamberdiev, Lei Gao
Summary: Legume plants establish symbiotic relationship with soil bacteria called rhizobia, and the expression of CLE peptides plays a crucial role in controlling the number of symbiotic nodules. The presence of nitrate affects the expression levels of other nodulation-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Mohammad Shahid, Udai B. B. Singh, Mohammad Saghir Khan, Prakash Singh, Ratan Kumar, Raj Narian Singh, Arun Kumar, Harsh V. V. Singh
Summary: Growth and productivity of crop plants worldwide are often adversely affected by stresses, both biotic and abiotic. Ethylene production in plants is induced by most stresses, which is detrimental to their growth and survival. Therefore, managing ethylene production in plants is becoming an attractive option for countering the negative effects on crop yield and productivity.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Ravinder K. Goyal, Maria Augusta Schmidt, Michael F. Hynes
Summary: The importance of biological nitrogen fixation has decreased with the widespread use of synthetic nitrogen fertilizers during the green revolution, due to their low cost and the lack of symbiotic associations with bacteria in staple crops. Recent advancements in symbiosis and nitrogen fixation, along with recombinant DNA technology, offer opportunities to increase symbiotically-driven nitrogen consumption globally.
Article
Biotechnology & Applied Microbiology
Rajnish Prakash Singh, Ying Ma, Afreen Shadan
Summary: The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) enzyme plays a crucial role in alleviating stress in plants by reducing ethylene production. Microbial ACCD can be used as a cost-effective and environmentally friendly biocatalyst for sustainable agriculture.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biology
Beatrice Lace, Chao Su, Daniel Invernot Perez, Marta Rodriguez-Franco, Tatiana Vernie, Morgane Batzenschlager, Sabrina Egli, Cheng-Wu Liu, Thomas Ott
Summary: Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the establishment of a functional Root Nodule Symbiosis (RNS). Comparative phylogenomic studies have identified RPG as a critical genetic determinant for bacterial infection. This study provides evidence that RPG is part of a protein complex involved in membrane polarization, cytoskeleton-mediated connectivity, and polar secretion during bacterial infection.
Article
Plant Sciences
Aung Htay Naing, Hui Yeong Jeong, Sung Keun Jung, Chang Kil Kim
Summary: Overexpression of the acdS gene in Petunia hybrida significantly reduced ethylene production under stress conditions, leading to greater stress tolerance in the transgenic plants. The improved stress tolerance was associated with better plant growth and physiological performance in the transgenics compared to the wild type plants. These results suggest that overexpression of acdS in ornamental plants can help overcome the detrimental effects of ethylene on plant growth under different abiotic stresses and benefit the floricultural industry.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Pooja Singh, Shiv Shanker Pandey, Basant Kumar Dubey, Rashmi Raj, Deepti Barnawal, Ashish Chandran, Laiq Ur Rahman
Summary: The study demonstrated that transgenic Geranium plants showed significant increases in plant height, biomass, leaf: stem ratio, and oil quantity compared to wild-type plants, as well as enhanced tolerance to salt and drought stress. Analysis of various parameters revealed that transgenic Geranium lines exhibited decreased ACC content under stress conditions, leading to increased ethylene production and improved response to salt and drought stress.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Review
Biochemistry & Molecular Biology
Dhanashree Vijayra Bomle, Asha Kiran, Jeevitha Kodihalli Kumar, Lavanya Senapathyhalli Nagaraj, Chamanahalli Kyathegowda Pradeep, Mohammad Azam Ansari, Saad Alghamdi, Ahmed Kabrah, Hamza Assaggaf, Anas S. Dablool, Mahadevamurthy Murali, Kestur Nagaraj Amruthesh, Arakere Chunchegowda Udayashankar, Siddapura Ramachandrappa Niranjana
Summary: Soil salinity stress is a major obstacle to food production worldwide, with strategies such as plant breeding and genetic engineering improving plant resistance to salinity stress to meet future food demand. The use of PGPR with ACC deaminase activity has shown promise in mitigating the impact of saline conditions and improving seed germination and seedling vigor. The variation of antioxidants and osmolytes in ACC deaminase-producing PGPR treated plants is also examined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Kemo Jin, Hongbo Li, Xiaoqing Li, Haigang Li, Ian C. Dodd, Andrey A. Belimov, William J. Davies, Jianbo Shen
Summary: This study found that inoculation with rhizobacteria can increase shoot biomass and root length density of maize under localized nutrient supply conditions, while also preventing the increase in root ethylene production, leading to enhanced fine root proliferation and nutrient uptake. The findings highlight the potential of regulating plant ethylene signaling through rhizosphere microorganisms for sustainable agriculture practices.
FOOD AND ENERGY SECURITY
(2021)
Article
Microbiology
Ajay Madhusudan Sorty, Fani Ntana, Martin Hansen, Peter Stougaard
Summary: Plants exposed to abiotic stress produce ACC, but plant growth-promoting bacteria (PGPB) can reduce ACC concentration. This study found that small molecules in wheat root exudates can influence the expression of the acdS and acdR genes in P. hormoni G20-18(T).
Article
Plant Sciences
Rocio Reyero-Saavedra, Sara Isabel Fuentes, Alfonso Leija, Gladys Jimenez-Nopala, Pablo Pelaez, Mario Ramirez, Lourdes Girard, Timothy G. Porch, Georgina Hernandez
Summary: This study aims to isolate and characterize symbiotic mutants in common bean (Phaseolus vulgaris) in order to understand the symbiotic nitrogen fixation (SNF) process in this important food crop. Three non-nodulating mutants were identified and their impairments in different symbiotic steps were characterized. The responsible mutated gene is being mapped for further understanding of SNF in common bean.
Article
Plant Sciences
Chaitanya Kumar Jha, Priyanka Sharma, Arpit Shukla, Paritosh Parmar, Rohit Patel, Dweipayan Goswami, Meenu Saraf
Summary: Ethylene has dual effects in plants, being harmful and beneficial, while microbes help reduce ethylene levels in plants by producing ACC deaminase, thereby promoting plant growth.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Microbiology
Francisco X. Nascimento, Anabel G. Hernandez, Bernard R. Glick, Marcio J. Rossi
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Maria J. Tavares, Ulrich Gueldener, Ana Mendes-Ferreira, Nuno P. Mira
Summary: Resequencing of S. ludwigii UTAD17 genome using PacBio resulted in 20 contigs totaling 13 Mb of assembled DNA, representing 95% of the DNA content. Annotation of the assembled genome predicted 4644 protein-encoding genes, including 213 proteins unique to this species.
Article
Environmental Sciences
Francisco X. Nascimento, Bernard R. Glick, Marcio J. Rossi
Summary: The study found that Achromobacter xylosoxidans SOLR10 and A. insolitus AB2 have plant hormone catabolic and plant growth promotion activities, and conducted genome analysis to reveal the genetic mechanisms behind their multiple plant hormone catabolism abilities. The inoculation of cucumber plants with these strains resulted in increased plant growth and development, indicating their beneficial properties. Additionally, genomic analysis showed the presence of various plant hormone catabolism gene clusters in the genomes of these strains, suggesting their role in plant-microbe interactions.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2021)
Article
Microbiology
Pedro R. Brandao, Maria T. B. Crespo, Francisco X. Nascimento
Summary: This study conducted a detailed analysis of the genus Komagataeibacter through phylogenomic analysis, revealing the phylogenomic relationships and important genes involved in cellulose biosynthesis. The results suggest the reclassification of several type strains as a new genus, Novacetimonas, and propose Novacetimonas hansenii species as the representative of this novel genus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Francisco X. Nascimento, Paola Uron, Bernard R. Glick, Admir Giachini, Marcio J. Rossi
Summary: Bacteria producing ACC deaminase can promote plant growth, enhance plant resistance to salt stress, and inhibit the growth of certain phytopathogens. The genome of these bacteria contains genetic elements involved in nutrient acquisition, stress tolerance, root exudate metabolism, and plant defense resistance, making them versatile in beneficial plant-microbe interactions.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Bernard R. Glick, Francisco X. Nascimento
Summary: The expression of ACC deaminase by plant-associated bacteria plays a crucial role in modulating plant ACC and ethylene concentrations, overcoming negative effects of inhibitory levels of ACC and ethylene on plant-microbe interactions, growth, and development. Pseudomonas strains with ACC deaminase activity are widely prevalent in the plant microbiome and are of interest for sustainable agricultural and biotechnological solutions. Understanding the properties and actions of ACC deaminase-producing Pseudomonas is key for enhancing plant growth, protecting plants from stress, and promoting rhizobial nodulation.
Article
Biotechnology & Applied Microbiology
Francisco Quintas-Nunes, Marcio J. Rossi, Francisco X. Nascimento
Summary: The study characterized and analyzed the genomic properties of a diazotrophic bacterium, Kosakonia radicincitans MUSA4, isolated from banana trees in Brazil. The strain exhibited several plant-growth-promoting traits and had the potential to significantly enhance cucumber plant growth. Detailed genomic analysis revealed the presence of genes associated with plant colonization, stress resistance, and nitrogen fixation in strain MUSA4. The strain also possessed strain-specific genes that may be linked to its evolutionary history in Brazilian mangrove environments.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
Karin Jelenko, Eva Cepec, Francisco X. Nascimento, Janja Trcek
Summary: Gluconacetobacter entanii is an acetic acid bacterium used in vinegar production. The previous strains of G. entanii have lost their culturable form, so we propose G. entanii AV429 as a new strain for future research.
Article
Plant Sciences
Francisco Quintas-Nunes, Pedro. R. R. Brandao, Maria. T. T. Barreto Crespo, Bernard. R. R. Glick, Francisco. X. X. Nascimento
Summary: This study presents a functional and genomic characterization of strain NFX-FRZ, a eukaryotic microalga belonging to the Micractinium genus, isolated from the rhizosphere of a plant in Portugal. The results show that strain NFX-FRZ belongs to a novel species, promotes tomato plant growth by synthesizing plant growth-promoting compounds and contains genes involved in phytohormone biosynthesis and signaling. This research provides insights into the importance of eukaryotic microalgae as plant growth-promoting agents and contributes to the understanding of phytohormone biosynthesis, signaling, and plant-microalgae interactions.
Article
Engineering, Chemical
Arooj Fatima, Paloma Ortiz-Albo, Luisa A. Neves, Francisco X. Nascimento, Joao G. Crespo
Summary: The production of bacterial cellulose as an alternative material has gained attention for its potential use in various processes and applications. This study focuses on producing custom bacterial cellulose membranes with relevant characteristics for air/gas filtration. Different bacterial strains were cultivated and characterized to determine their suitability for producing cellulose membranes. The resulting membranes were freeze-dried and extensively characterized, showing distinctive morphological properties. The cultivation conditions affected the membrane properties and gas filtration behavior. The membranes demonstrated adequate mechanical properties and permeance for N2, CO2, and O2, suggesting their potential use in air/gas filtration.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Microbiology
Constanca D. F. Bertrand, Rodrigo Martins, Francisco Quintas-Nunes, Pedro Reynolds-Brandao, Maria T. B. Crespo, Francisco X. Nascimento
Summary: This study reports the characterization and genomic analysis of Saccharopolyspora sp. NFXS83, a marine bacterium isolated from seawater from the Sado estuary in Portugal. The NFXS83 strain produced multiple functional and stable extracellular enzymes under high-salt conditions, showed the ability to synthesize auxins such as indole-3-acetic acid, and produced diffusible secondary metabolites capable of inhibiting the growth of Staphylococcus aureus. These findings indicate that Saccharopolyspora sp. NFXS83 has a significant potential for a wide range of marine biotechnological applications.
Article
Engineering, Electrical & Electronic
Guilherme B. Pintarelli, C. T. S. Ramos, J. R. da Silva, M. J. Rossi, D. O. H. Suzuki
Summary: The study investigated the effects of irreversible electroporation on wine yeast and methods of verification. It was found that irreversible electroporation can effectively control yeast numbers without compromising food palatability under specific conditions.
IEEE SENSORS JOURNAL
(2021)