Article
Plant Sciences
Fabienne Gehrke, Paola Ruiz-Duarte, Angelina Schindele, Sebastian Wolf, Holger Puchta
Summary: The application of the CRISPR/Cas system as a biotechnological tool for genome editing has revolutionized plant biology. Recently, the repertoire was expanded by CRISPR-Kill, enabling CRISPR/Cas-mediated tissue engineering through genome elimination by tissue-specific expression. This new system not only provides spatial control by tissue-specific expression but also allows temporal control of CRISPR-mediated cell death in Arabidopsis thaliana.
Review
Biochemistry & Molecular Biology
Yingxiao Zhang, Brian Iaffaldano, Yiping Qi
Summary: CRISPR-derived biotechnologies have revolutionized genetic engineering in plants, but still face challenges such as editing efficiency and off-target mutagenesis.
PLANT COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Aryaman Girish Patwardhan, Sateesh Belemkar
Summary: Alzheimer's disease is a chronic illness with pathological hallmarks of amyloid plaques and neurofibrillary tangles, genetic predisposition, and current treatments being mainly palliative. New therapeutic approaches like stem cell therapy and gene editing are seen as promising directions for the future.
Review
Cell & Tissue Engineering
Gabrielle M. Robbins, Minjing Wang, Emily J. Pomeroy, Branden S. Moriarity
Summary: NK cells have the potential to be a crucial part of cancer immunotherapy, but face challenges such as in vivo persistence and the tumor microenvironment. Enhancing NK cell resistance to tumor inhibitory signaling through genome engineering may improve their efficacy in cancer treatment.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Gemma Llargues-Sistac, Laia Bonjoch, Sergi Castellvi-Bel
Summary: The use of next-generation sequencing (NGS) technologies has greatly advanced our understanding of the mutational landscape of complex human diseases such as cancer. Haploid human cell models, such as the HAP1 cell line, have emerged as valuable tools for functional gene studies, especially in combination with CRISPR-Cas9 gene editing technology. This review explores the recent applications of the HAP1 cell line in functional genetic studies and high-throughput genetic screens, highlighting its potential to enhance our understanding of gene function and the genetic basis of human diseases identified through NGS technologies, and its implications for clinical practice and patient care.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Genetics & Heredity
Zohreh Harsij, Zahra Ghafoorzadeh, Elahe Goharian
Summary: A biotechnological revolution is triggered by the versatility, accuracy, and efficiency of CRISPR-Cas systems. Diagnosing nucleic acid biomarkers using CRISPR is a highly sensitive method. It can detect a wide range of infectious and non-infectious diseases, mutations, and CRISPR deletions associated with genetic disorders. Additionally, this technology has applications in protein and micromolecule testing. However, despite its many contributions to biomedical science, CRISPR technology has negative impacts on the health of living organisms, environmental conditions, and population structures.
Review
Chemistry, Physical
Noshad Peyravian, Maziar Malekzadeh Kebria, Jafar Kiani, Peiman Brouki Milan, Masoud Mozafari
Summary: This review focuses on the recent advancements of microfluidic systems to deliver CRISPR/Cas systems in clinical and therapy investigations, highlighting the limitations of conventional delivery tools and the advantages of microfluidic systems in in vitro delivery.
Article
Multidisciplinary Sciences
Chong Li, Jonas Simon Fleck, Catarina Martins-Costa, Thomas R. Burkard, Jan Themann, Marlene Stuempflen, Angela Maria Peer, Abel Vertesy, Jamie B. Littleboy, Christopher Esk, Ulrich Elling, Gregor Kasprian, Nina S. Corsini, Barbara Treutlein, Juergen A. Knoblich
Summary: The study develops a high-throughput CRISPR screening system in cerebral organoids to identify vulnerable cell types and gene regulatory networks associated with autism spectrum disorder. The researchers found that perturbing members of the chromatin remodelling complex can affect cell fate determination in progenitor cells.
Review
Biotechnology & Applied Microbiology
Dongdong Zhao, Xinna Zhu, Hang Zhou, Naxin Sun, Ting Wang, Changhao Bi, Xueli Zhang
Summary: Effective metabolic pathways are crucial for efficient cell factories, and utilizing CRISPR technology in pathway engineering can enhance efficiency, reduce costs, and simplify target customization. This technology has been proven to be robust and effective in various organisms.
METABOLIC ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Beata Balla, Florin Tripon, Claudia Banescu
Summary: Genome engineering allows precise manipulation of DNA sequences in cells, starting from Meganucleases to more advanced tools like ZFNs, TALENs, and CRISPR. CRISPR, guided by RNA recognition and precise DNA cleavage, has revolutionized genome engineering with applications in epigenetics and functional genomics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Ana Paula Mulet, Magdalena Ripoll, Lorena Betancor
Summary: Biocatalysis has emerged as a promising alternative to conventional chemical processes, and CRISPR-based technologies have the potential to enhance whole-cell bacterial biotransformations for more sustainable production of industrially important products.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Review
Cell Biology
Maysam Mansouri, Martin Fussenegger
Summary: Cell therapy approaches utilizing engineered mammalian cells for on-demand production of therapeutic agents in the patient's body have advanced beyond proof-of-concept in translational medicine. Customized therapeutic cells can sense user-defined signals, process them, and respond programmably. Various methods of controlling cell behavior, including open-loop and closed-loop systems, are discussed, along with therapeutic applications of engineered cells in early disease diagnosis and treatment. Emerging technologies like digital devices also show potential for future incorporation into cell-based therapies.
Article
Oncology
Ingunn M. Stromnes, Ayaka Hulbert, Meagan R. Rollins, Ryan S. Basom, Jeffrey Delrow, Patrick Bonson, Adam L. Burrack, Sunil R. Hingorani, Philip D. Greenberg
Summary: This study investigates the role of immune checkpoints in mediating functional dysfunction of engineered T cells in pancreatic ductal adenocarcinoma (PDA). The findings suggest that blockade of PD-1 signaling alone is not enough to overcome the dysfunction of TCR engineered T cells in PDA. Contributions from both the differentiation pathways induced during the T cell engineering process and intratumoral suppressive mechanisms render engineered T cells dysfunctional.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Review
Engineering, Biomedical
Diana Stoycheva, Hasan Simsek, Willi Weber, Anja E. Hauser, Enrico Klotzsch
Summary: B cells play a promising role in immunotherapy for cancer and autoimmune diseases. Understanding the development, functions, and interactions with the microenvironment of B cells is crucial for the development of targeted therapies.
ACTA BIOMATERIALIA
(2021)
Article
Oncology
Haibo Sun, Thomas G. Martin, John Marra, Denice Kong, Jonathon Keats, Sandrine Mace, Marielle Chiron, Jeffrey L. Wolf, Jeffrey M. Venstrom, Raja Rajalingam
Summary: The study revealed that specific genetic makeup of patients can influence their response to Isa-lenalidomide-dexamethasone therapy, with patients carrying KIR3DL2 and HLA-A3/11 plus high-affinity FCGR3A-158V allele showing improved progression-free survival. Conversely, patients with KIR2DL1 and HLA-C2C2 combination may have a lower response to this treatment.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
