期刊
ANALYTICAL CHEMISTRY
卷 91, 期 4, 页码 2847-2853出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b04792
关键词
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资金
- Walter C. Sumner Memorial Fellowship
- Natural Sciences and Engineering Research Council (Postgraduate Scholarship-Doctoral Program)
- Canadian Institutes of Health Research [FDN-148415]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2016-06090]
The spread of antibiotic-resistant bacteria poses a global threat to public health. Conventional bacterial detection and identification methods often require pre-enrichment and/or sample preprocessing and purification steps that can prolong diagnosis by days. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most widespread antibiotic-resistant bacteria and is the leading cause of hospital-acquired infections. Here, we have developed a method to specifically capture and detect MRSA directly from patient nasal swabs with no prior culture and minimal processing steps using a microfluidic device and antibody-functionalized magnetic nanoparticles. Bacteria are captured based on antibody recognition of a membrane-bound protein marker that confers beta-lactam antibiotic resistance. MRSA identification is then achieved by the use of a strain-specific antibody functionalized with alkaline phosphatase for electrochemical detection. This approach ensures that only those bacteria of the target strain and resistance profile are measured. The method has a limit of detection of 845 CFU/mL and excellent discrimination against high concentrations of common nontarget nasal flora with a turnaround time of under 4.5 h. This detection method was successfully validated using clinical nasal swab specimens (n = 30) and has the potenuill to be tailored to various bacterial targets.
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