Article
Plant Sciences
Jiajia Wang, Liang Cao, Xia Gao, Huifang Gao, Xialin Chen, Zhenzhong Wang, Wei Xiao
Summary: This study investigated the chemical composition and pharmacokinetics of Jiuwei Xifeng granules (JWXF) and found that it contains 75 components, including alkaloids, terpenes, and organic acids. The main compounds, gastrodin, gentiopicroside, and sweroside, showed rapid absorption, elimination, and dose-dependent pharmacokinetics in rats.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Plant Sciences
Fan He, Yu-Feng Huang, Wei Dai, Xian-You Qu, Jing-Guang Lu, Chi-Chou Lao, Wen-Hui Luo, Dong-Mei Sun, Mei Wei, Sheng-Yuan Xiao, Ying Xie, Liang Liu, Hua Zhou
Summary: In this study, the TOF-SIMS image analysis technology was used for the first time to detect Chinese herbal medicine. Taking Coptis rhizome as an example, the distribution of nine active compounds in the cross-section of Coptis rhizome was studied. The results showed that these compounds had different distribution patterns in the cross-section and their contents increased with the growth years.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Tinglan Zhang, Jun Zhang, Fangfang Chen, An Liu, Jinzhu Jiang, Zhiyong Yan, Xianju Liu
Summary: In this study, we analyzed the triterpenoid compounds in different fresh tissues of Pulsatilla chinensis using UHPLC-Q-TOF-MS and UHPLC-QQQ-MS techniques. A total of 132 triterpenoids were identified, including new aglycones and new ways of rhamnose linking. We established an analytical method for triterpenoid content and found significant distribution differences in types and contents of triterpenoids among different tissues. We also identified 15 differential components between aboveground and underground tissues. This study provides an efficient analysis strategy for triterpenoids in P. chinensis and important insights into the biosynthetic pathway of triterpenoid saponins.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Applied
Ajay Anand, Anusha Komati, Kumar Katragunta, Hussain Shaik, Narendra Kumar Nagendla, Madhusudana Kuncha, Mohana Krishna Reddy Mudiam, Katragadda Suresh Babu, Ashok Kumar Tiwari
Summary: Boiled rhizome of Nymphaea nouchali shows significant antihyperglycemic and antioxidative stress potentials, making it a promising food material for managing hyperglycemia and oxidative stress.
Article
Plant Sciences
Guanghou Wang, Hongjin Wang, Ziwei Lin, Lijuan Hou, Jia Yue Wang, Lixin Sun
Summary: This study aimed to investigate the phytochemical profile of alkaloids in N. officinalis total alkaloids and determine their pharmacokinetic behaviors. The results showed that 55 alkaloids were identified in N. officinalis total alkaloids, with 25 alkaloids absorbed by rats after oral administration. The established LC-ESI-MS/MS method was successfully applied for the simultaneous determination of major active alkaloids in rat plasma, providing valuable insights for pre-clinical and clinical investigations.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Chemistry, Applied
Xi Juan Zhao, Peng Mei Guo, Wen Hui Pang, Tao Tan, Yao Hai Zhang, Bi Ning Jiao
Summary: This study investigated the secondary metabolites of Jindou kumquat and identified the dominant characteristic metabolites. The findings provide a basis for further evaluation and utilization of Fortunella germplasms.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Agriculture, Multidisciplinary
Karolina Tkacz, Angel Gil-Izquierdo, Sonia Medina, Igor Piotr Turkiewicz, Raul Dominguez-Perles, Paulina Nowicka, Aneta Wojdylo
Summary: Sea buckthorn juice is rich in phytochemicals such as oxylipins, amino acids, and carotenoids, which have potential health benefits including anti-cholinergic, anti-diabetic, anti-obesity, anti-inflammatory, and antioxidant properties. Selective correlation between enzyme inhibition and antioxidant activity suggests the importance of choosing juice from specific berry cultivars for consumers seeking specific health benefits.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Food Science & Technology
Yibin Lan, Min Liu, Xinke Zhang, Siyu Li, Ying Shi, Changqing Duan
Summary: The environmental conditions of wine regions determine the chemical composition and sensory characteristics of young Marselan wines. Using analytical methods such as gas chromatography-mass spectrometry and high-performance liquid chromatography, it was found that the concentrations of volatile compounds can differentiate young Marselan wines from different regions. The compounds and sensory attributes of Marselan wines are related to the specific environmental conditions of the wine-producing regions.
Article
Pharmacology & Pharmacy
Junyi Bai, Tingting Xiong, Xiao Wang, Yanfen Cheng, Ruifeng Luo, Xiangdong Yang, Chaomei Fu
Summary: This study investigates the therapeutic effects and potential mechanisms of Lian-Zhi-Fan (LZF) decoction in the treatment of ulcerative colitis (UC) using a metabolomics approach. The results demonstrate that LZF ameliorates weight loss, disease activity index (DAI) score, and colon inflammation in UC rats. Metabolomics analysis identifies 14 potential biomarkers, five of which have diagnostic significance. These biomarkers are mainly involved in amino acid metabolism, fat metabolism, and energy production.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Dong-Shin Kim, Sun Lee, Suk Man Park, Su Hyun Yun, Han-Seung Gab, Sang Suk Kim, Hyun-Jin Kim
Summary: The study analyzed 10 citrus varieties to evaluate their chemical profiles and antioxidant activities, finding significant variations in content of sugars, organic acids, flavonoids, limonoids, and carotenoids among different varieties. These differences led to variations in antioxidant capacity and quality parameters. The study provides valuable information for understanding the relationship between citrus quality and metabolite profiles, which can be useful for the development and improvement of new citrus varieties.
Article
Chemistry, Applied
Liuwei Zhao, Caijun Ren, Xiaofeng Xue, Huanxian Lu, Kai Wang, Liming Wu
Summary: This study characterized monofloral safflower honey (MSH) from a metabolomic perspective and proposed safflomin A as a reliable chemical marker for its authentication. Commercial non-safflower honeys did not test positive for safflomin A, indicating its potential as a fingerprinting chemical marker for safflower honey authentication.
Article
Chemistry, Analytical
Yiwen Huang, Hui Lu, Zhong Li, Yuan Zeng, Qi Xu, Yang Wu
Summary: Ursodeoxycholic acid has gained attention for its preventive effect on SARS-CoV-2 infection. A novel gradient RP-HPLC method coupled to CAD was developed for the simultaneous separation and quantification of nine impurities in ursodeoxycholic acid, with high sensitivity and reliability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Laximan Velip, Vivek Dhiman, Bhoopendra Singh Kushwah, Vijaya Madhyanapu Golla, S. Gananadhamu
Summary: Urapidil, an antihypertensive drug, was investigated under various stress conditions according to ICH guidelines. A stability indicating HPLC method and LC-Q-TOF-MS were used to separate, identify, and evaluate the toxicity of five impurities. The study findings are important for understanding the stability of the drug and the mechanism of impurity formation.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Dian Liu, Yang Han, Han Zhou, Hongli Jin, Hongjian Kang, FeiFei Huang, Yanfang Liu, Xinmiao Liang
Summary: The study introduced an offline preparative three-dimensional HPLC method to efficiently purify alkaloids, successfully isolating 24 indole alkaloids from Gelsemium elegans Benth. including four new alkaloids and one first-identified compound. This method is not only effective for systematic purification of alkaloids from this plant but also has the potential for alkaloid preparation from other plants.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Yu Yang, Yanan Zhang, Caiyan He, Mengyuan Xie, Huitai Luo, Ying Wang, Jun Zhang
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2018)
Article
Chemistry, Analytical
Luo Hui-Tai, Huang Xiao-Lan, Wu Hui-Qin, Zhu Zhi-Xin, Huang Fang, Lin Xiao-Shan
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2012)
Article
Biochemical Research Methods
Xi Zhou, Huiqin Wu, Xiaolan Huang, Fang Hang, Huitai Luo
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2019)
Article
Chemistry, Applied
Xi Zhou, Huiqin Wu, Xiaolan Huang, Fang Hang, Huitai Luo
Article
Chemistry, Analytical
Bin Xie, Huitai Luo, Xiaolan Huang, Fang Huang, Qiuyan Zhang, Xuefeng Wu, Xi Zhou, Huiqin Wu
Summary: This study validated a simple, quick, sensitive, and reliable method for simultaneous quantification of six 2-(2-phenylethyl) chromones in rat plasma after oral administration of agarwood ethanol extract. The method showed good linearity, precision, accuracy, and stability of the analytes. This validated method could serve as a reference for further pharmacodynamic research and clinical applications of agarwood.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Bin Xie, Xue-Feng Wu, Hui-Tai Luo, Xiao-Lan Huang, Fang Huang, Qiu-Yan Zhang, Xi Zhou, Hui-Qin Wu
Summary: This study identified and characterized 73 non-volatile constituents of Pogostemon cablin Benth using HPLC-Q-TOF-MS, and classified the samples into herbal decoction pieces and processed medicinal materials. Potential chemical markers were screened to distinguish the two groups, providing valuable information for further quality evaluation of PCB.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Luo Hui-Tai, Huang Xiao-Lan, Wu Hui-Qin, Zhang Qiu-Yan, Zhu Zhi-Xin, Huang Fang, Lin Xiao-Shan
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Luo Huitai, Huang Xiaolan, Wu Huiqin, Zhang Qiuyan, Zhu Zhixin, Huang Fang, Lin Xiaoshan, Ma Yefen, Deng Xin
CHINESE JOURNAL OF CHROMATOGRAPHY
(2017)
Article
Chemistry, Analytical
Luo Huitai, Huang Xiaolan, Wu Huiqin, Zhu Zhixin, Huang Fang, Lin Xiaoshan, Zhang Qiuyan, Ma Yefen
CHINESE JOURNAL OF CHROMATOGRAPHY
(2016)
Article
Chemistry, Analytical
Luo Huitai, Xie Mengting, Huang Xiaolan, Wu Huiqin, Zhu Zhixin, Huang Fang, Lin Xiaoshan, Ouyang Gangfeng
CHINESE JOURNAL OF CHROMATOGRAPHY
(2015)
Article
Chemistry, Analytical
Yang Yun-Yun, Deng Jie-Wei, Luo Hui-Tai, Wu Qing-Hui, Guo Peng-Ran
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2010)
Article
Chemistry, Analytical
Huang Xiao-Lan, Luo Hui-Tai, Wu Hui-Qin, Chen Wen-Rui, Zou Zhi-Fei, Huang Fang, Lin Xiao-Shan, Zhu Zhi-Xin
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2009)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)