期刊
ANALYTICA CHIMICA ACTA
卷 1048, 期 -, 页码 50-57出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.10.018
关键词
Immunoassay; Tetrabromobisphenol A; Derivative; Tetrabromobisphenol A bis(2-hydroxyethyl) ether; Electrochemical competitive immunosensor
资金
- National Natural Science Foundation of China [21577051, 21407066]
- Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
A competitive immunosensor was established using an electrochemical amperometric strategy for sensitive detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE), an important derivative of tetrabromobisphenol A (TBBPA). In this system, the amplified electrochemical signal towards the reduction of hydrogen peroxide (H2O2) was recorded by amperometric method. Meanwhile, the synthetized catalase functionalized AuNPs-loaded self-assembled polymer nanospheres showed an excellent electrocatalytic ability to catalyse H2O2, which was beneficial for strengthening the electrochemical signals. Under the optimized conditions, this method displayed: (i) low detection limits (0.12 ng/mL, 7 times lower than the traditional ELISA with the same antibody); (ii) satisfactory accuracy (recoveries, 78-124%; RSD, 2.1-8.3%) and good agreement with the corresponding ELISA; (iii) low sample consumption (6 mu L) and low cost. The proposed approach was applied for investigation of TBBPA-DHEE from environmental waters, and our results indicated that this immunosensor has great potential to detect the trace pollutants in aquatic environments. (C) 2018 Elsevier B.V. All rights reserved.
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