Article
Biochemistry & Molecular Biology
Hyunbum Kim, Laurensia Danis Anggradita, Sun-Jae Lee, Sung Sik Hur, Joonsuk Bae, Nathaniel Suk-Yeon Hwang, Seung Min Nam, Yongsung Hwang
Summary: This study revealed the molecular characteristics and high fibrotic activity of keloid dermal fibroblasts at cell-matrix interfaces. Additionally, downregulation of anabolic collagen-cross-linking enzymes through epidermal growth factor led to decreased fibrotic phenotypes and cell motility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Lucia Sanjurjo, Iris A. Schulkens, Pauline Touarin, Roy Heusschen, Ed Aanhane, Kitty C. M. Castricum, Tanja D. De Gruijl, Ulf J. Nilsson, Hakon Leffler, Arjan W. Griffioen, Latifa Elantak, Rory R. Koenen, Victor L. J. L. Thijssen
Summary: The study reveals that specific chemokine-galectin interactions can modulate the immunoregulatory function of galectins, affecting their glycan-binding activity and specificity. In particular, binding of CXCL4 changes the carbohydrate binding site of galectin-1, increasing its apoptotic activity on CD8(+) T cells.
COMMUNICATIONS BIOLOGY
(2021)
Review
Cell Biology
Maike Kuemper, Joy Steinkamp, Paola Zigrino
Summary: This review focuses on the regulation of metalloproteinases in maintaining skin homeostasis, particularly in dermal homeostasis. It discusses their activity and expression regulation mechanisms and how the dysregulation of these metalloproteinases affects disease development, particularly cancer and aging. Furthermore, it emphasizes recent discoveries and new therapeutic approaches that target metalloproteinase activity, as well as the potential of naturally derived components in regulating metalloproteinase expression and activity in disease.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Medical Laboratory Technology
Priscilla Stela Santana de Oliveira, Pablo Ramon Gualberto Cardoso, Simao Kalebe de Paula Silva, Angela Luzia Branco Pinto Duarte, Michelle Melgarejo da Rosa, Moacyr Jesus Barreto de Melo Rego, Michelly Cristiny Pereira, Ivan da Rocha Pitta, Maira Galdino da Rocha Pitta
Summary: Galectin-1 and 4 levels were found to be significantly higher in osteoarthritis patients compared to healthy controls, with galectin-1 showing even higher levels in patients with joint edema. Surprisingly, galectin-4 seems to play a role in the inflammation process of osteoarthritis, similar to the well-known galectin-1.
CLINICAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zelie Dirand, Marion Tissot, Brice Chatelain, Celine Viennet, Gwenael Rolin
Summary: The study suggests that 3D spheroid culture can effectively downregulate the fibrogenic features of keloid fibroblasts, making it an efficient model for studying the deactivation of fibrotic cells but not suitable for studying fibrogenesis in keloid.
Article
Peripheral Vascular Disease
Mika Sawane, Yuki Ogura, Ayano Nakamura, Hitomi Otaka, Hiroyasu Kidoya, Katsujiro Sato, Nobuyuki Takakura, Kentaro Kajiya
Summary: The study found that appropriate stiffness induces vascular maturation and upregulated APJ expression, while overexpression of APJ promotes the formation of large vessels, potentially providing a novel mechanism for preventing human skin aging.
Article
Biochemistry & Molecular Biology
Yi-Kai Hong, Chen-Han Wu, Yu-Chen Lin, Yu-Lun Huang, Kuo-Shu Hung, Tsung-Pin Pai, Yen-Ting Liu, Tzu-Chi Chen, Hardy Chan, Chao-Kai Hsu
Summary: This study demonstrated that ASC-J9 treatment of keloid fibroblasts can inhibit the activation of fibroblasts, reduce cell division, inflammation, ROS generation, and extracellular matrix synthesis, as well as suppress IL-6 protein secretion. Inhibiting the ROS/STAT3/IL-6 axis by ASC-J9 may represent a potential therapeutic approach to suppress cell proliferation and ECM production in keloids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Maria J. Moure, Ana Gimeno, Sandra Delgado, Tammo Diercks, Geert-Jan Boons, Jesus Jimenez-Barbero, Ana Arda
Summary: The method utilizes C-13 labeling of specific monosaccharide units to disrupt NMR chemical shift degeneracy, allowing for precise identification of the unique binding epitope within sugar oligomers when binding to protein receptors.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Virology
Jose Miguel Urra, Elisa Ferreras-Colino, Marinela Contreras, Carmen M. Cabrera, Isabel G. Fernandez de Mera, Margarita Villar, Alejandro Cabezas-Cruz, Christian Gortazar, Jose de la Fuente
Summary: The immune response to alpha-Gal may contribute to the control of COVID-19 by altering antibody titers and isotype composition to assess disease severity.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Surgery
Jingzhe Yang, Pingyang Deng, Yonggang Qi, Xinshu Feng, Hailing Wen, Fengping Chen
Summary: NEAT1 is upregulated in keloid tissues and fibroblasts, while miR-196b-5p is downregulated. NEAT1 suppresses cell viability and migration in keloid fibroblasts by regulating the miR-196b-5p/FGF2 axis.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Shuang Lin, Xiaoning He, Yuanjia He
Summary: The co-culture of ASCs/EPCs enhances gene expression related to skin and angiogenesis, and accelerates the repair of skin defects in vivo. This approach may offer a promising alternative to traditional single-seed cell therapy for vascularized skin regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Laura Duffy, John Henderson, Max Brown, Stefan Pryzborski, Nicola Fullard, Lena Summa, Jorg H. W. Distler, Richard Stratton, Steven O'Reilly
Summary: The study revealed that overexpression of Gremlin-1 activates quiescent cells to myofibroblasts and leads to functional effects. Gremlin-1 induces this process by increasing TGF-beta 1 levels and SBE luciferase activity. Inhibition of Gremlin-1 reduces collagen levels, indicating its key role in systemic sclerosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dermatology
Kevin Serror, Lauren Ferrero, Francoise Boismal, Maxime Sintes, Manuel Thery, Benoit Vianay, Emilie Henry, David Gentien, Pierre DE LA Grange, David Boccara, Maurice Mimoun, Jean-David Bouaziz, Armand Benssussan, Laurence Michel
Summary: Keloid scars are hypertrophic and proliferating pathological scars with no tendency to regression. There is heterogeneity in keloid morphologies, as well as within a keloid itself, particularly in the expression of ECM-associated genes, proliferation, migration, and traction forces of fibroblasts. This heterogeneity leads to a better understanding of keloid pathophysiology and treatment adaptation.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Medicine, Research & Experimental
Grayson Hatfield, Lioudmila Tepliakova, Jessica Tran, Huixin Lu, Michel Gilbert, Roger Y. Y. Tam
Summary: Monoclonal antibody (mAb) production using non-human cells can introduce non-human glycan epitopes, leading to potential allergic reactions. This study focuses on the binding of anti-a1-3-Gal IgE antibodies to the Fc region of mAbs with varying degrees of a1-3-galactosylation. The results suggest that bi-a1-3-galactosylation is a critical quality attribute for mAb-based biotherapeutics.
Review
Pharmacology & Pharmacy
Leyla Norouzi-Barough, Ardeshir Bayat
Summary: Validation of potential drug targets is crucial in discovering and developing new therapies for dermal fibrotic conditions like keloid disease. Integration of multi-omics data with traditional and modern technological approaches offers unique opportunities for identifying and validating novel targets in the early stages of drug development.
DRUG DISCOVERY TODAY
(2021)