Article
Biology
Zeyu Li, Mengdi Li, Jianbo Wang
Summary: This study analyzed the changes in chromatin accessibility and its relationship with epigenetic modifications and gene expression during the process of allopolyploidization in Brassica napus. The results showed that there was a shock in chromatin accessibility in both allopolyploidization and natural evolutionary processes, and gene accessible chromatin regions (ACRs) increased after allopolyploidization. ACRs associated with H3K27ac or H3K4me3 modifications were more accessible than those associated with H3K27me3. Furthermore, the subgenome C-n exhibited higher chromatin accessibility compared to A(n), which was mainly due to the higher chromatin accessibility of C-n-unique genes.
COMMUNICATIONS BIOLOGY
(2022)
Review
Plant Sciences
Elizabeth Ihien Katche, Annaliese S. Mason
Summary: Resynthesized rapeseed lines have recreated the historical hybridization between Brassica rapa and B. oleracea, providing valuable genetic resources for rapeseed breeding and insights into polyploid plant meiosis evolution. This review discusses the production of these lines through interspecific crosses, their resulting fertility and agronomically important traits, meiotic instability issues, and the potential role of meiosis genes and gene regulatory networks in stabilizing meiosis. The direct use of resynthesized and semi-resynthesized Brassica napus for hybrid breeding and the study of other agronomic traits is also explored.
CRITICAL REVIEWS IN PLANT SCIENCES
(2023)
Article
Plant Sciences
Dingfan Luo, Desheng Mei, Wenliang Wei, Jia Liu
Summary: This study provides a detailed analysis of R2R3-MYB genes in rapeseed, uncovering information about their structure, evolutionary relationships, chromosome locations, gene structures, protein structures, cis-acting promoter elements, and gene duplications. The study reveals that BnaMYB genes have undergone segmental duplications and positive selection pressure. Genes within the same subfamily share similar intron-exon patterns and motifs. Additionally, cis-element analysis identified numerous drought-responsive and other stress-responsive elements in the promoter regions of the BnaMYB genes. Four genes were identified as significant upregulated under drought stress, potentially serving as important drought-responsive genes. These findings lay a foundation for understanding the complex mechanisms of BnaMYB in multiple developmental stages and pathways related to drought stress in rapeseed.
Article
Plant Sciences
Guoqiang Zheng, Xiaoyun Dong, Jiaping Wei, Zigang Liu, Ali Aslam, JunMei Cui, Hui Li, Ying Wang, Haiyan Tian, Xiaodong Cao
Summary: In this study, the DNA methylation pattern and its regulated pathway of winter rapeseed under freezing stress were explored using methylome and transcriptome analysis. The results showed that the average methylation level decreased under freezing stress, with CG methylation level being the highest. Functional enrichment analysis identified several differentially expressed genes associated with differentially methylated genes, including core transcription factors involved in plant response to stress.
Article
Multidisciplinary Sciences
Sundas Tanveer, Nosheen Akhtar, Noshin Ilyas, R. Z. Sayyed, Betty Natalie Fitriatin, Kahkashan Perveen, Najat A. Bukhari
Summary: This research examines the effects of Pseudomonas putida and salicylic acid on the growth of canola under stress and non-stress conditions. The combined application of Pseudomonas putida and salicylic acid significantly improves the plant's growth and stress tolerance. They have significant effects on germination and various morphological, physiological, and biochemical parameters.
Article
Biochemistry & Molecular Biology
Jian Li, Yujing Duan, Nianli Sun, Lu Wang, Shanshan Feng, Yujie Fang, Youping Wang
Summary: The study revealed that miR169n acts as a negative regulator of drought resistance in rapeseed by targeting the NF-YA8 gene. The target mimicry construct MIM169n enhanced the resistance of transgenic plants to drought stress, leading to improved growth and antioxidative capability.
Article
Plant Sciences
Meimei Hu, Zengde Xi, Jianbo Wang
Summary: This study utilized transcriptome, WGBS, and Chip-Seq sequencing data to explore the regulatory consequences of DNA methylation and histone modifications on gene expression in allotetraploid Brasscia napus. The results demonstrated that DNA methylation is crucial for gene expression regulation, and different epigenetic modifications have an essential function in regulating the differential expression of genes in B. napus.
Article
Plant Sciences
Franz Boideau, Gautier Richard, Olivier Coriton, Virginie Huteau, Caroline Belser, Gwenaelle Deniot, Frederique Eber, Cyril Falentin, Julie Ferreira de Carvalho, Marie Gilet, Maryse Lode-Taburel, Loeiz Maillet, Jerome Morice, Gwenn Trotoux, Jean-Marc Aury, Anne-Marie Chevre, Mathieu Rousseau-Gueutin
Summary: Meiotic recombination is an important evolutionary process in sexual organisms that generates genetic diversity. This process is tightly regulated, with most crossovers occurring in chromosomal regions with low DNA methylation levels. However, there are still islands without recombination in these regions, which can be caused by DNA methylation or structural variations.
Article
Biochemistry & Molecular Biology
Cuiping Zhang, Lijing Lu, Ruolin Gong, Xing Su, Fengbo Liu, Ru Zhang, Jihong Hu
Summary: Trihelix (TH) proteins, as plant-specific transcription factors, play important roles in plant growth, development, and stress responses. However, the function of TH genes in Brassica napus and their evolution and differentiation patterns in Brassica plants are still unclear. In this study, we identified and classified 455 TH genes in Brassica species and Arabidopsis. The analyses revealed the conservation and variation of TH protein motifs and gene structures. The expansion of TH genes in tetraploid species was found to be due to hetero-tetraploidization and gene duplication events. Comparative analysis showed significant expansion of GT-2 and SIP1 genes during evolution. Differential expression of BnaTH genes under various stresses suggests their involvement in stress responses. The presence of stress-responsive cis-elements in the promoter region indicates the potential of BnaTH genes to cope with diverse environments. qRT-PCR analyses confirmed the response of TH genes to abiotic stresses. These findings provide valuable information and candidate genes for further studying the role of TH genes in stress resistance of B. napus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zahoor Ahmad, Shazia Anjum, Milan Skalicky, Ejaz Ahmad Waraich, Rana Muhammad Sabir Tariq, Muhammad Ashar Ayub, Akbar Hossain, Mohamed M. Hassan, Marian Brestic, Mohammad Sohidul Islam, Muhammad Habib-Ur-Rahman, Allah Wasaya, Muhammad Aamir Iqbal, Ayman El Sabagh
Summary: The application of selenium can improve the yield and physiological-biochemical attributes of camelina and canola crops, alleviating the adverse effects of drought stress.
Article
Plant Sciences
Muhammad Azhar Hussain, Dan Luo, Liu Zeng, Xiaoyu Ding, Yong Cheng, Xiling Zou, Yan Lv, Guangyuan Lu
Summary: This study explores the gene expression profiles of Brassica napus in response to low temperature stress using transcriptome data. The study identifies the enriched biological functions and metabolic pathways in response to low temperature stress and summarizes the expression patterns of transcription factors in this response. It also reveals a complex regulatory network in these pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Muhammad Ahsan Farooq, Faisal Islam, Ahsan Ayyaz, Weiqi Chen, Yamna Noor, Weizhen Hu, Fakhir Hannan, Weijun Zhou
Summary: MT-Se NPs application can enhance plant growth and reduce oxidative stress under As stress, showing potential for synergistic effects and improved enzymatic activities in plants.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Yan Yu, Jia Dong, Rui Li, Xue Zhao, Zonghe Zhu, Fugui Zhang, Kejin Zhou, Xianyong Lin
Summary: Hydrogen sulfide (H2S) plays important regulatory roles in plant growth and stress responses. This study shows that application of H2S donor or scavenger can alleviate the inhibition of rapeseed root growth caused by aluminum (Al) stress. Exogenous H2S improves aluminum tolerance by controlling H2S and ROS accumulation and enhancing aluminum exclusion through replenishing antioxidant reservoirs and facilitating pectin methylation. Further investigation is needed to understand the regulation of endogenous H2S levels and improve plant stress tolerance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Plant Sciences
Jin Hu, Mudan Luo, Xianming Zhou, Zhaoyang Wang, Li Yan, Dengfeng Hong, Guangsheng Yang, Xiaohui Zhang
Summary: This study identified a novel regulatory gene, BnaJUL1, and its target gene BnaTBCC1, which play important roles in drought tolerance in rapeseed. The findings provide valuable germplasm for breeding drought-tolerant rapeseed.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Horticulture
Naveen Naveen, Nisha Kumari, Ram Avtar, Minakshi Jattan, Sushil Ahlawat, Babita Rani, Kamla Malik, Anubhuti Sharma, Manjeet Singh
Summary: The study demonstrates that under drought stress conditions, brassinolide can enhance the physio-biochemical attributes by improving the antioxidant system and photosynthetic efficiency in drought-tolerant cultivars, while reducing oxidative stress indicators such as hydrogen peroxide.
Article
Plant Sciences
Kun Du, Yuyue Xiao, Qier Liu, Xinyue Wu, Jinjin Jiang, Jian Wu, Yujie Fang, Yang Xiang, Youping Wang
PLANT CELL REPORTS
(2019)
Letter
Biotechnology & Applied Microbiology
Jian Wu, Chen Chen, Guiyu Xian, Dongxiao Liu, Li Lin, Shengliang Yin, Qinfu Sun, Yujie Fang, Hui Zhang, Youping Wang
PLANT BIOTECHNOLOGY JOURNAL
(2020)
Article
Plant Sciences
Guangqin Cai, Chuchuan Fan, Sheng Liu, Qingyong Yang, Dongxu Liu, Jian Wu, Jianwu Li, Yongming Zhou, Liang Guo, Xuemin Wang
Article
Plant Sciences
Muhammad Uzair Qasim, Qing Zhao, Muhammad Shahid, Rana Abdul Samad, Sunny Ahmar, Jian Wu, Chuchuan Fan, Yongming Zhou
FRONTIERS IN PLANT SCIENCE
(2020)
Review
Biochemistry & Molecular Biology
Li Lin, Jian Wu, Mingyi Jiang, Youping Wang
Summary: Global warming and population growth have increased the need for plants to rescue themselves in unfavorable environments to meet food requirements. Stress signal sensing plays a crucial role in determining the appropriate response and ultimately the survival of plants. Plant mitogen-activated protein kinase (MAPK) cascades are key in regulating responses to major environmental stresses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jian Li, Keyun Lin, Shuai Zhang, Jian Wu, Yujie Fang, Youping Wang
Summary: This study conducted a genome-wide analysis of MYB-related transcription factors in rapeseed, identifying 251 BnMYB members and revealing their phylogenetic and evolutionary patterns. The research demonstrated the enrichment of important cis-acting regulatory elements in the promoter regions of BnMYB-related genes. By studying stress-responsive MYB-related genes, potential candidate genes for genetic improvement of rapeseed were identified.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Huizhen Hu, Yiwei Tang, Jian Wu, Feizhi Chen, Yidan Yang, Xuancheng Pan, Xiang Dong, Xianda Jin, Sheng Liu, Xuezhu Du
Summary: By investigating the role of BnMED16 in Brassica napus's defense mechanism against the fungal pathogen Sclerotinia sclerotiorum, it was found that BnMED16 enhances multiple signaling pathways to increase the plant's resistance, leading to activation of defense-related genes and hormone-responsive pathways, resulting in enhanced defense capabilities.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Dongxiao Liu, Jian Wu, Li Lin, Panpan Li, Saifen Li, Yue Wang, Jian Li, Qinfu Sun, Jiansheng Liang, Youping Wang
Summary: The study revealed that a Sclerotinia-resistant line of Brassica napus showed better xylem development and higher lignin content in the stems compared to a susceptible line, aiding in limiting the invasion and spread of S. sclerotiorum. Overexpression of the gene BnaC.CCR2.b increased lignin content in the stems and enhanced resistance to the pathogen. Increasing lignin content in the stems of B. napus proves to be an important strategy in controlling Sclerotinia.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Hongxia Fan, Wenwen Yang, Jiayue Nie, Chen Lin, Jian Wu, Dewei Wu, Youping Wang
Summary: The study characterized a YML079-like cupin protein (SsYCP1) from Sclerotinia sclerotiorum, showing its potential role as an effector protein contributing to the pathogenicity of the pathogen. Overexpression of SsYCP1 promoted infection, while silencing the gene inhibited it, suggesting a novel role for YML079-like cupin proteins in plant-pathogen interactions.
Article
Microbiology
Hongxia Fan, Wenwen Yang, Jiayue Nie, Wenjuan Zhang, Jian Wu, Dewei Wu, Youping Wang
Summary: A novel effector, SsERP1, was identified in Sclerotinia sclerotiorum, which promotes infection by inhibiting the plant ethylene signaling pathway. Knocking down SsERP1 effectively inhibits Sclerotinia sclerotiorum infection, suggesting a potential strategy for disease control.
Article
Plant Sciences
Li Lin, Jialin Fan, Panpan Li, Dongxiao Liu, Sichao Ren, Keyun Lin, Yujie Fang, Chen Lin, Youping Wang, Jian Wu
Summary: Sclerotinia stem rot (SSR) is one of the most devastating diseases in Brassica napus. Traditional breeding for resistance is challenging due to limited resistant germplasm. In this study, a S. sclerotiorum-inducible promoter was developed for engineering SSR-resistant Brassica crops.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Letter
Plant Sciences
Jian Wu, Huimin Liu, Sichao Ren, Panpan Li, Xue Li, Li Lin, Qinfu Sun, Long Zhang, Chen Lin, Youping Wang
Article
Biology
Kun Du, Yang Yang, Jinping Li, Ming Wang, Jinjin Jiang, Jian Wu, Yujie Fang, Yang Xiang, Youping Wang
Summary: Phosphorus stress is a significant factor limiting plant growth, and miRNA, specifically miR399, plays a role in regulating the response to nutrient stress. This study showed that overexpressing Bna-miR399c in rapeseed increased taproot length, lateral root number, biomass, and phosphorus accumulation while reducing anthocyanin content and improving chlorophyll content under low phosphorus stress. BnPHO2 was identified as one of the targets of Bna-miR399c, and its overexpression increased phosphorus rejection in rapeseed seedlings. This research provides insights into improving phosphorus uptake and transportation in rapeseed and has potential applications in germplasm innovation and the development of intelligent crops.
Article
Agronomy
Jian Wu, Shengliang Yin, Li Lin, Dongxiao Liu, Sichao Ren, Wenjing Zhang, Wencheng Meng, Peipei Chen, Qinfu Sun, Yujie Fang, Cunxu Wei, Youping Wang
Summary: In this study, host-induced gene silencing (HIGS) technology based on RNA interference (RNAi) was successfully applied to protect oilseed rape from S. sclerotiorum infection. By targeting three pathogenicity genes, stable transgenic B. napus plants were generated, resulting in the suppression of fungal growth and improved resistance to S. sclerotiorum.
Article
Agronomy
Jian Wu, Peipei Chen, Qing Zhao, Guangqin Cai, Yue Hu, Yang Xiang, Qingyong Yang, Youping Wang, Yongming Zhou
