期刊
ACS NANO
卷 13, 期 5, 页码 5810-5815出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.9b01408
关键词
biohybrid nanostructures; phages; His tag; self-assembly; enzyme recovery; enzymatic micropumps
类别
资金
- Volkswagen Stiftung
- DFG as part of the project SPP 1726 [253407113]
- BW Stiftung [BioMatS-10]
- Max Planck Society
Catalytically active colloids are model systems for chemical motors and active matter. It is desirable to replace the inorganic catalysts and the toxic fuels that are often used with biocompatible enzymatic reactions. However, compared to inorganic catalysts, enzyme-coated colloids tend to exhibit less activity. Here, we show that the self-assembly of genetically engineered M13 bacteriophages that bind enzymes to magnetic beads ensures high and localized enzymatic activity. These phage-decorated colloids provide a proteinaceous environment for directed enzyme immobilization. The magnetic properties of the colloidal carrier particle permit repeated enzyme recovery from a reaction solution, while the enzymatic activity is retained. Moreover, localizing the phage-based construct with a magnetic field in a microcontainer allows the enzyme-phage-colloids to function as an enzymatic micropump, where the enzymatic reaction generates a fluid flow. This system shows the fastest fluid flow reported to date by a biocompatible enzymatic micropump. In addition, it is functional in complex media including blood, where the enzyme-driven micropump can be powered at the physiological blood-urea concentrations.
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