Article
Biochemistry & Molecular Biology
Emmanuel Baudouin, Juliette Puyaubert, Patrice Meimoun, Melisande Blein-Nicolas, Marlene Davanture, Michel Zivy, Christophe Bailly
Summary: This study investigated the remodeling of Arabidopsis seed phosphoproteome during imbibition using stable isotope dimethyl labeling and nanoLC-MS/MS analysis. The study identified a large repertoire of phosphopeptides and phosphoproteins involved in seed germination control, including protein kinases and key regulators like DOG1. Gene ontology analysis revealed their involvement in various biological processes. The study provides evidence of the major role of phosphosignaling in the regulation of Arabidopsis seed germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Michelle D'Aguillo, Kathleen Donohue
Summary: The timing of developmental transition can influence subsequent life stages. Habitat tracking through phenology can alter selection on postgermination traits. The results support the theoretical predictions of feedbacks between habitat tracking and traits expressed after habitat selection.
Article
Plant Sciences
Chengliang Wang, Yuanyuan Lyu, Qi Zhang, Hongye Guo, Defu Chen, Xiwen Chen
Summary: This study reports a glycosylphosphatidylinositol-anchored beta-1,3-glucanase, BG14, which degrades callose in seed embryos and plays important roles in seed longevity and dormancy in Arabidopsis.
Article
Biochemistry & Molecular Biology
Xu Wang, Marlene Davanture, Michel Zivy, Christophe Bailly, Eiji Nambara, Francoise Corbineau
Summary: This study demonstrates the importance of the proteolytic N-degron pathway in ethylene-mediated changes in seed proteomes during germination in Arabidopsis thaliana.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Valerie Delorme-Hinoux, Assane Mbodj, Sophie Brando, Anne De Bures, Christel Llauro, Fabrice Covato, Joseph Garrigue, Claude Guisset, Jacques Borrut, Marie Mirouze, Jean-Philippe Reichheld, Julio Saez-Vasquez
Summary: The transcription of 45S rDNA is crucial for ribosome biogenesis, and the Arabidopsis thaliana genome contains multiple units of 45S rDNA. However, not all units are transcribed, and there are variations in rRNA genes and ribosomes within the 45S rDNA units. A study conducted on Arabidopsis thaliana samples from different altitudes in France revealed dynamic copy numbers of 45S rDNA and identified new genotypes for the External Transcribed Spacers (ETS) regions. Interestingly, there were altitude-specific differences in genotype diversity for 5' ETS and sea level-specific differences for 3' ETS. The structural analysis also showed the preservation of specific sequences/features required for rDNA expression and rRNA processing. This study highlights the importance of natural diversity in 45S rDNA and its potential for altering ribosome composition.
Article
Plant Sciences
Jia-Xing Guo, Ru-Feng Song, Kai-Kai Lu, Yu Zhang, Hui-Hui Chen, Jia-Xin Zuo, Ting-Ting Li, Xue-Feng Li, Wen-Cheng Liu
Summary: The C-type cyclin protein CycC1;1 interacts with ABI5 to downregulate ABA-responsive genes and ABI5 itself, thereby inhibiting ABA signaling during seed germination.
Article
Plant Sciences
Hagai Shohat, Hadar Cheriker, Amir Cohen, David Weiss
Summary: The plant hormone abscisic acid (ABA) regulates seed maturation, dormancy, and germination under stress conditions. ABA-IMPORTING TRANSPORTER 1.1 (AIT1.1) in tomato is involved in radicle emergence under salinity. AIT1.1 upregulation after seed imbibition promotes ABA uptake, restricts endosperm weakening, and enhances resistance to ABA. The ait1.1 mutant shows improved germination and partial resistance to ABA under high salinity, suggesting its potential use in improving seed germination in crops.
Article
Plant Sciences
Sher Ney Chong, Pratibha Ravindran, Prakash P. Kumar
Summary: DOF6 induces MLP329 expression to enhance primary seed dormancy in Arabidopsis via a high ABA/GA ratio, and this is suppressed by RGL2 in a negative-feedback mechanism to regulate dormancy. MLP329 probably enhances the endogenous ABA/GA ratio to positively regulate primary seed dormancy.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Lipeng Zheng, Masahiko Otani, Yuri Kanno, Mitsunori Seo, Yushi Yoshitake, Kohki Yoshimoto, Kazuhiko Sugimoto, Naoto Kawakami
Summary: Seed dormancy is an adaptive trait that allows plants to survive adverse conditions and restart growth at suitable times. Controlling seed dormancy is important for crop production and food quality. The study found that SFL1 is a positive regulator of seed dormancy, affecting the initiation and termination of the dormancy program.
Article
Agriculture, Multidisciplinary
Yumin Zhang, Dan Li, Lynnette M. A. Dirk, A. Bruce Downie, Tianyong Zhao
Summary: ZmAGA1 overexpression in Arabidopsis reduces RFOs and galactinol content in seeds, leading to increased seed germination percentage under stress conditions, but decreased seed aging tolerance. Manipulation of ZmAGA1 expression can potentially regulate maize seed germination and aging tolerance simultaneously.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Yongtao Han, Elisabeth Georgii, Santiago Priego-Cubero, Christoph J. Wurm, Patrick Huether, Gregor Huber, Robert Koller, Claude Becker, Joerg Durner, Christian Lindermayr
Summary: Seed dormancy is a crucial developmental transition that affects plant adaptation and survival. In this study, it was found that the loss of function of two plant-specific histone deacetylases, HD2A and HD2B, enhanced seed dormancy in Arabidopsis. Silencing of HD2A and HD2B resulted in hyperacetylation of the DOG1 locus and increased DOG1 expression during seed maturation and imbibition. The study also revealed the interaction between HD2A, HD2B, HSI2, and HSL1. Overall, these findings suggest that HSI2 and HSL1 may recruit HD2A and HD2B to negatively regulate DOG1 expression and reduce seed dormancy, thus affecting seed development and germination.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Jiabao Huang, Lin Yang, Liu Yang, Xiaoyu Wu, Xiaoshuang Cui, Lili Zhang, Jiyun Hui, Yumei Zhao, Hongmin Yang, Shangjia Liu, Quanling Xu, Maoxuan Pang, Xinping Guo, Yunyun Cao, Yu Chen, Xinru Ren, Jinzhi Lv, Jianqiang Yu, Junyi Ding, Gang Xu, Nian Wang, Xiaochun Wei, Qinghui Lin, Yuxiang Yuan, Xiaowei Zhang, Chaozhi Ma, Cheng Dai, Pengwei Wang, Yongchao Wang, Fei Cheng, Weiqing Zeng, Ravishankar Palanivelu, Hen-Ming Wu, Xiansheng Zhang, Alice Y. Cheung, Qiaohong Duan
Summary: Flowering plants have developed self-incompatibility and interspecific barriers to prevent the production of unfavorable offspring. Self-incompatibility is achieved by the interaction between S-Locus proteins and S-Locus receptor kinases to reject self-pollen, while interspecific barriers follow the rule of unilateral incompatibility on self-incompatible pistils and unilateral compatibility on self-compatible pistils. In Brassicaceae, S-Locus proteins and S-Locus receptor kinases activate FER-mediated reactive oxygen species production to reject incompatible pollen, while diverged pollen coat proteins trigger nitric oxide to facilitate pollen growth in a preferential manner, maintaining species integrity.
Article
Plant Sciences
Cun-Zhi Jia, Jing-Jing Wang, Da-Li Chen, Xiao-Wen Hu
Summary: This research examined the seed dormancy, germination, and soil seed bank dynamics of Eruca sativa, a versatile cruciferous species. The study found that E. sativa seeds can germinate in a wide temperature range and exhibit non-deep physiological dormancy at maturity. The germination of seeds is sensitive to water and salinity stress. When buried in soil, the seeds form a persistent soil seed bank, but emergence is hindered when buried deeper than 5 cm. This species' germination characteristics allow it to adapt to the harsh low-temperature climate of the Qinghai-Tibet Plateau.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xu Wang, Malaika Maraya Gomes, Christophe Bailly, Eiji Nambara, Francoise Corbineau
Summary: The study revealed that PRT6 plays a role in ethylene-induced dormancy release in Arabidopsis thaliana seeds, with RAPs acting downstream of PRT6 and HREs acting upstream. Ethylene promotes germination by down-regulating dormancy-related genes in the GA and ABA signaling pathways, while the insensitivity of prt6 seeds to ethylene is mainly due to disturbances in GA signaling.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Plant Sciences
Domenique Andre, Jose Alfredo Zambrano, Bo Zhang, Keh Chien Lee, Mark Ruhl, Alice Marcon, Ove Nilsson
Summary: SHORT VEGETATIVE PHASE (SVP) is a negative regulator of flowering and represses FT transcription. In poplar trees, an SVP ortholog called SVL is involved in regulating growth cessation, bud set, and dormancy in response to short day and temperature cues. SVL represses FT2 transcription through binding to its promoter and decreases the amount of active GAs by repressing the transcription of GA20 oxidase.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Zoology
Brianne Edwards, Liana T. Burghardt, Katherine E. Kovach, Kathleen Donohue
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Liana T. Burghardt, Brendan Epstein, Joseph Guhlin, Matt S. Nelson, Margaret R. Taylor, Nevin D. Young, Michael J. Sadowsky, Peter Tiffin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Microbiology
Brendan Epstein, Reda A. Abou-Shanab, Abdelaal Shamseldin, Margaret R. Taylor, Joseph Guhlin, Liana T. Burghardt, Matthew Nelson, Michael J. Sadowsky, Peter Tiffin
Article
Ecology
Bradley J. Tomasek, Liana T. Burghardt, Robert K. Shriver
Article
Ecology
Liana T. Burghardt, Brendan Epstein, Peter Tiffin
Review
Plant Sciences
Liana T. Burghardt
Article
Plant Sciences
Liana T. Burghardt, Diana Trujillo, Brendan Epstein, Peter Tiffin, Nevin D. Young
Article
Biotechnology & Applied Microbiology
Liana T. Burghardt, Brendan Epstein, Michelle Hoge, Diana I. Trujillo, Peter Tiffin
Summary: The research examines the influence of environmental factors on the nitrogen-fixing mutualism between leguminous plants and rhizobial bacteria. It finds that host genotype, nitrogen addition, rhizobial density, and community complexity all affect selection on rhizobial strains. Rhizobial density has a stronger impact on strain frequency compared to nitrogen addition. Higher density leads to less diverse but more beneficial nodule communities, particularly in the context of more selective host genotypes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Brendan Epstein, Liana T. Burghardt, Katy D. Heath, Michael A. Grillo, Adam Kostanecki, Tuomas Hamala, Nevin D. Young, Peter Tiffin
Summary: This study investigated the coevolution between legumes and rhizobia using population genomic analysis. The results showed that there were genotype-by-genotype effects on rhizobial fitness and some rhizobial genes displayed signatures of recent positive selection. However, neither annotated host symbiosis genes nor genes responsible for this variation showed enrichment for positive or balancing selection. This suggests that stabilizing selection dominates the selection acting on symbiotic traits, and the variation in these traits is under mutation-selection balance. Furthermore, the study found that the symbiosis may not be a major driver of variation in plant growth in multistrain contexts.
Article
Biology
Rebecca T. Batstone, Liana T. Burghardt, Katy D. Heath
Summary: This study examines the (co)evolution of host-associated microbiomes and finds that microbial and host fitness tend to trade-off rather than generating conflict. The results highlight the importance of quantifying microbial relative fitness for understanding microbiome evolution and improving host fitness.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Review
Microbiology
Liana Burghardt, George C. diCenzo
Summary: Rhizobial bacteria have diverse habitats and engage in competitive interactions in different environments. Advanced measurement tools and sequencing technologies have been utilized to study competition mechanisms in plant systems. However, there is still limited knowledge about competition in certain environments. An ecological framework can provide a better understanding of the evolutionary ecology of rhizobia and facilitate the development of sustainable beneficial associations with hosts.
CURRENT OPINION IN MICROBIOLOGY
(2023)
Article
Ecology
Alejandra Martinez-Berdeja, Miki Okada, Martha D. D. Cooper, Daniel E. E. Runcie, Liana T. T. Burghardt, Johanna Schmitt
Summary: The timing of seasonal rains affects phenology and fitness of plant populations in Mediterranean climates. In this study, Arabidopsis thaliana was used to investigate the effects of rainfall onset on germination phenology and fitness. Results showed that germination timing was influenced by the timing of rainfall onset, and the fitness of the plants was affected by germination timing. High dormancy and early flowering genotypes had a fitness advantage in a Mediterranean climate.
FUNCTIONAL ECOLOGY
(2023)
Article
Plant Sciences
Mannix Burns, Brendan Epstein, Liana T. Burghardt
Summary: Leguminous plants have symbiotic relationships with rhizobia, with other bacterial endophytes also inhabiting nodules. Genetic variation within legume hosts can have direct and indirect effects on the establishment of nodule microbiomes. Further studies can help engineer and optimize nodule endophyte communities for beneficial qualities.
PHYTOBIOMES JOURNAL
(2021)
Editorial Material
Plant Sciences
Liana T. Burghardt
Meeting Abstract
Zoology
Kathleen Donohue, Brianne Edwards, Liana Burghardt
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2017)