期刊
BIOELECTROCHEMISTRY
卷 101, 期 -, 页码 1-7出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.06.007
关键词
Glucose biosensor; Self-assembled monolayer; Amperometcy; Impedance spectroscopy; Langmuir-Blodgett films
资金
- Japan Society for the Promotion of Science (ISPS) [2136006]
- Faculty of Marine Technology, Tokyo University of Marine Science and Technology
A novel glucose biosensor with an immobilized mediator was studied using electrochemical impedance spectroscopy (EIS) and amperometry measurements. The biosensor has a characteristic ultrathin form and is composed of a self-assembled monolayer anchoring glucose oxidase (GOx) covered with Langmuir-Blodgett (LB) films of Prussian blue (PB). The immobilized PB in the LB films acts as a mediator and enables the biosensor to work under a low potential (0.0 V vs. Ag/AgCI). In the EIS measurements, a dramatic decrease in charge transfer resistance (R-ct) was observed with sequential addition of glucose, which can be attributed to enzymatic activity. The linearity of the biosensor response was observed by the variation of the sensor response (1/R-ct) as a function of glucose concentration in the range 0 to 25 mM. The sensor also showed linear amperometric response below 130 mM glucose. The organic-inorganic system of GOx and PB nanodusters demonstrated bifunctional sensing action, both amperometry and EIS modes, as well as long sensing stability for 4 days. (C) 2014 Elsevier B.V. All rights reserved.
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