Article
Biotechnology & Applied Microbiology
Kaylanne Montenegro, Claudia Flores, Ana Paula Alves Nascimento, Beatriz Oliveira Farias, Andressa S. Goncalves Brito, Mariana Magaldi, Aline Gimenez, Ivano de Filippis, Maysa Mandetta Clementino, Kayo Bianco, Enrico Saggioro, Paulo Barrocas
Summary: The occurrence and clonal relationships of carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital and municipal wastewater treatment plants (WWTPs) were investigated in this study. The results showed that among the 18 Kl. pneumoniae strains recovered, 39% were classified as multidrug-resistant (MDR), 61.1% were extensively drug-resistant (XDR), and 83.3% exhibited carbapenemase activity. Three carbapenemase-encoding genes (bla(KPC), bla(NDM), and bla(OXA-370)) were identified, and five sequence types (ST11, ST37, ST147, ST244, and ST281) were determined. It is important to monitor antimicrobial resistance in WWTPs effluents and use advanced treatment technologies to minimize the spread of bacterial load and antibiotic resistance genes in aquatic ecosystems.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Infectious Diseases
Constantinos Tsioutis, Vanessa M. Eichel, Nico T. Mutters
Summary: This critical review aimed to evaluate the effectiveness of measures to control the spread of KPC-KP in healthcare settings, finding that successful control during outbreaks doesn't necessarily indicate the effectiveness of individual infection control measures, due to low-quality evidence and lack of controlled intervention studies. The distribution of studies, reported cases, and high mortality rates highlight that KPC-KP remains a major healthcare problem globally.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Jee Hong Kim, Yun Young Cho, Ji Young Choi, Yu Mi Wi, Kwan Soo Ko
Summary: This study investigated the characteristics of KPC-2-producing Klebsiella pneumoniae isolates from a hospital in South Korea. It identified two main clones, ST11 and ST307, with different resistance profiles and virulence levels. Continuous monitoring of these clones, which show different characteristics including resistance and virulence, is necessary.
Article
Multidisciplinary Sciences
Thitiya Yungyuen, Piriyaporn Chongtrakool, Wanatpreeya Phongsamart, Unchalee Puangprasart, Chalermsri Pummangura, Dararat Samretwit, Somporn Srifuengfung
Summary: Through the study of enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), we characterized the clonal relationships among 62 Streptococcus pneumoniae serotype 19A isolates from different patients in 21 hospitals. Comparing the results with multilocus sequence types (MLST), we found that ERIC-PCR is a valuable complementary tool for the investigation of S. pneumoniae serotype 19A isolates. However, there is less concordance between these two methods.
Article
Pharmacology & Pharmacy
Qiuxia Lin, Menglu Wu, Hanbing Yu, Xiaojiong Jia, Hua Zou, Deyu Ma, Siqiang Niu, Shifeng Huang
Summary: In Chongqing, China, the study revealed that CRE infections were associated with high mortality rates and poor outcomes, with CPE patients more likely to exhibit antibiotic resistance. Independent predictors for CPE infection were ICU admission and hepatobiliary system diseases. Although there was no significant difference in desirability of outcome ranking (DOOR) outcomes between the two groups, CPE patients had prolonged ICU stays.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
Lin Huang, Min Cao, Yanyan Hu, Rong Zhang, Yufei Xiao, Gongxiang Chen
Summary: The trend of fosfomycin resistance among KPC-KP strains is increasing in China, with the two major mechanisms of resistance being plasmid-mediated fosfomycin resistance gene fosA3 and mutation of the target gene glpT.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Ana Claudia Souza Rodrigues, Marilene Rodrigues Chang, Ivson Cassiano de Oliveira Santos, Ana Paula D'Alincourt Carvalho-Assef
Summary: This study investigated the molecular epidemiology of blaKPC-encoding Klebsiella pneumoniae in three public hospitals in Brazil, revealing various resistance genes, new clone types, and the spread of K. pneumoniae among different hospitals in the same region.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Agriculture, Dairy & Animal Science
Tianle Xu, Xinyue Wu, Hainan Cao, Tianxu Pei, Yu Zhou, Yi Yang, Zhangping Yang
Summary: This study investigated the distribution of virulence gene expression and antimicrobial susceptibility in Klebsiella pneumoniae isolated from dairy cows with clinical mastitis in Jiangsu Province. The study revealed potential links between virulence gene expression and antimicrobial resistance. The results provide important insights into the spread of infection by K. pneumoniae and resistance in the case of bovine mastitis, as well as potential target genes involved in the links between virulence determinants and multidrug resistance.
Article
Microbiology
Jacqueline Findlay, Laurent Poirel, Mario Juhas, Patrice Nordmann
Summary: This study evaluated the impact of KPC variants on CZA resistance, finding increased susceptibility to cephalosporins and carbapenems but increased resistance to ceftazidime and piperacillin-tazobactam. The KPC variants exhibited increased affinity towards ceftazidime and slightly decreased sensitivity to avibactam, impacting CZA resistance while concurrently negatively impacting carbapenemase activities.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Public, Environmental & Occupational Health
Tian Wei, Chengyun Zou, Jie Qin, Jianmin Tao, Li Yan, Jiangjun Wang, Hong Du, Fang Shen, Yanqin Zhao, Haiying Wang
Summary: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) infections in older patients present clinical and molecular characteristics that require attention. The presence of resistance and virulence genes pose challenges for treatment selection and infection control measures.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Eva Gato, Paula Guijarro-Sanchez, Isaac Alonso-Garcia, Rosa Pedraza-Merino, Adrian Conde, Emilio Lence, Soraya Rumbo-Feal, Andrea Pena-Escolano, Cristina Lasarte-Monterrubio, Tania Blanco-Martin, Ana Fernandez-Gonzalez, M. del Carmen Fernandez-Lopez, Romina Maceiras, Marta Martinez-Guitian, Juan Carlos Vazquez-Ucha, Luis Martinez-Martinez, Concepcion Gonzalez-Bello, Jorge Arca-Suarez, Alejandro Beceiro, German Bou
Summary: By studying high-risk Klebsiella pneumoniae strains, we found that imipenem/relebactam was more effective than ceftazidime/avibactam in preventing resistance mutations. In some strains, imipenem/relebactam resistance developed faster and reached higher levels compared to ceftazidime/avibactam. Lineages that evolved in the presence of ceftazidime/avibactam showed KPC substitutions associated with high-level resistance, while lineages that evolved in the presence of imipenem/relebactam showed specific substitutions related to imipenem/relebactam resistance.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Qiwei Chen, Lizhang Liu, Xiaofang Hu, Xu Jia, Xiaowei Gong, Youjun Feng, Man Huang
Summary: The study focuses on the diversity of KPC-2-producing plasmids in highly transmissible ST11 clones of carbapenem-resistant K. pneumoniae (CRKP). The researchers report a clinical case of a rare ST437 CRKP isolate, K186, which produces KPC-2. Whole-genome sequencing revealed three distinct resistance plasmids, one of which, pK186_KPC, is a small IncN-type plasmid of 26,012bp. The study further suggests that the carbapenem-resistant pK186_KPC might have arisen from the integration of ancestral IncN and IncFII plasmids.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Lucia Henrici De Angelis, Noemi Poerio, Vincenzo Di Pilato, Federica De Santis, Alberto Antonelli, Maria Cristina Thaller, Maurizio Fraziano, Gian Maria Rossolini, Marco Maria D'Andrea
Summary: Phage therapy for bacterial infections is being reconsidered, with a focus on understanding the molecular targets of phages. In a study on resistance to the lytic phage phi BO1E in Klebsiella pneumoniae, a mutant with a single mutation in the CPS biosynthesis gene was found to be less virulent, indicating a potential benefit of phage resistance for the host in the context of phage-bacteria-host interactions.
Article
Infectious Diseases
Yao Zhai, Daixi Li, Pengcheng Du, Zhao Zhang, Zilong He, Yatao Guo, Yusheng Chen, Yu Kang, Songnian Hu, Zhancheng Gao
Summary: The study identified two new KPC-harbouring plasmids in Klebsiella pneumoniae isolates from a hospital in Fujian, China. These plasmids carried multiple antibiotic resistance genes and were mainly found in sequence type 11 K. pneumoniae isolates. Analysis indicated a high level of similarity among the sequenced isolates, suggesting potential clonal dissemination of KPC-producing K. pneumoniae. The study highlights the importance of plasmid analysis in monitoring and controlling antibiotic resistance spread.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
Hong-Fu Li, Li-Xia Zhang, Wen-Lan Zhang, Jing Li, Ya-Qian Li, Tong-Ping Hu
Summary: The clinical, microbiological and molecular epidemiological characteristics of Klebsiella pneumoniae (KPN) caused pyogenic liver abscess (PLA) in Inner Mongolia, China were analysed in this study. The results showed a higher detection rate of KPN in the puncture fluid compared to blood and urine samples. The dominant KPN sequence type (ST) in the Baotou region was found to be ST23. This research provides valuable insights into the virulence and treatment of PLA caused by KPN.
INFECTION AND DRUG RESISTANCE
(2023)