期刊
FRONTIERS IN CHEMISTRY
卷 6, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2018.00657
关键词
chitosan grafted with beta-cyclodextrin; antimicrobial activity; membrane integrity; uptake; solubility
资金
- National Natural Science Foundation of China [31802228]
- University of Nursing Programs for Young Scholars with Creative Talents in Heilongjiang Province [UNPYSCT-2016133]
- Northeastern Science Inspection Station [DY201702]
- China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology [DY201702]
- Program for Science and Technology Research of China [2015BAD11B03-07]
- earmarked fund for China Agriculture Research System-35
We synthesized chitosan grafted with beta-cyclodextrin (CD-g-CS) from mono-6-deoxy-6-(p-toluenesulfonyl)-beta-cyclodextrin and chitosan. Two different amounts of immobilized beta-cyclodextrin (beta-CD) on CD-g-CS (Q(CD): 0.643 x 10(3) and 0.6 x 10(2) mu mol/g) were investigated. The results showed that the amino contents of CD-g-CS with Q(CD) = 0.643 x 10(3) and 0.6 x 10(2) mu mol/g were 6.34 +/- 0.072 and 9.41 +/- 0.055%, respectively. Agar diffusion bioassay revealed that CD-g-CS (Q(CD) = 0.6 x 10(2) mu mol/g) was more active against Staphylococcus xylosus and Escherichia coli than CD-g-CS (Q(CD) = 0.643 x 10(3) mu mol/g). Cell membrane integrity tests and scanning electron microscopy observation revealed that the antimicrobial activity of CD-g-CS was attributed to membrane disruption and cell lysis. Uptake tests showed that CD-g-CS promoted the uptake of doxorubicin hydrochloride by S. xylosus, particularly for CD-g-CS with Q(CD) = 0.6 x 10(2) mu mol/g, and the effect was concentration dependent. CD-g-CS (Q(CD) = 0.6 x 10(2) and 0.643 x 10(3) mu mol/g) also improved the aqueous solubilities of sulfadiazine, sulfamonomethoxine, and sulfamethoxazole. These findings provide a clear understanding of CD-g-CS and are of great importance for reducing the dosage of antibiotics and antibiotic residues in animal-derived foods. The results also provide a reliable, direct, and scientific theoretical basis for its wide application in the livestock industry.
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