4.4 Article

Methane Production from the Co-digestion of Algal Biomass with Crude Glycerol by Anaerobic Mixed Cultures

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WASTE AND BIOMASS VALORIZATION
卷 11, 期 5, 页码 1873-1881

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SPRINGER
DOI: 10.1007/s12649-018-0542-0

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Microalgae; Biodiesel waste; Anaerobic digestion; Biogas

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The optimization of factors affecting methane production from the co-digestion of algal biomass with crude glycerol was investigated using response surface methodology with a central composite design. The investigated parameters included algal biomass, crude glycerol and inoculum concentration. The inoculum and algal biomass concentration had a significant individual effect on the methane production (p <= 0.05). The interactive effect on methane production was found to be between algal biomass and crude glycerol (p <= 0.05). The optimal conditions were 20.02 g-VS/L of crude glycerol, 9.76 g-VS/L of inoculum concentration and 5.50 g-VS/L of algal biomass. Under optimum conditions, the maximum methane production of 58.88 mL-CH4/L was obtained. The difference between the observed methane production (58.88 mL-CH4/L) and the predicted methane (68.94 mL-CH4/L) was 14.59%. Under the optimum conditions, the energy production of 92.47 J/g-VSadded was obtained. The polymerase chain reaction-denaturing gradient gel electrophoresis analysis indicated that the methane producers present in the fermentation broth were Methanosarcina sp., Methanoregula sp., Methanospirillum sp. and Methanoculleus sp.

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