Article
Fisheries
B. Lukman, M. N. Roslindawani, M. Azzam-Sayuti, M. A. Norfarrah, S. Annas, M. Y. Ina-Salwany, M. Zamri-Saad, A. R. Nor-Yasmin, S. Amin-Nordin, T. Barkham, M. N. A. Amal
Summary: Tilapia lake virus (TiLV) causes infection in tilapia and co-infection with Streptococcus agalactiae leads to higher mortality. This study compares the severity of single and co-infection, finding that co-infection increases mortality and disease severity in red hybrid tilapia.
Article
Fisheries
Raja Swaminathan Thangaraj, Sundar Raj Nithianantham, Lekshmi Narendrakumar, Tina Kollannoor Johny, Neeraj Sood, Pravata Kumar Pradhan, Kuldeep Kumar Lal
Summary: The experimental results showed that Indian cichlids are not susceptible to TiLV infection, as confirmed by the absence of clinical signs or TiLV-specific amplification in both in vivo and in vitro tests.
Article
Fisheries
Pravata Kumar Pradhan, Dev Kumar Verma, Shrish Chandra Yadav, Atul Krishna Dev, Thangaraj Raja Swaminathan, Anutosh Paria, Rajendran Kooloth Valappil, Neeraj Sood
Summary: After experimental infection with Tilapia tilapinevirus, it was found that Catla, Mrigal, and Silver carp are resistant to TiLV infection and unlikely to be carriers of the virus.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Entomology
Kirsten Warcup, Alex S. Torson, George D. Yocum
Summary: "Research on Sex Determination Methods of Colorado Potato Beetles" used publicly available RNA-seq data to select 5 candidate genes for sex-specific markers in adult Colorado potato beetles. The study confirmed that these 5 marker candidates exhibit a sex-specific expression pattern and can be used as PCR markers for sex determination. This method allows for simple sex detection using cDNA as the template and assigning sex to RNA-seq samples post hoc.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Plant Sciences
Shaonpius Mondal, Laura Jenkins Hladky, William M. Wintermantel
Summary: Viruses transmitted by the whitefly pose a growing threat to cucurbit production worldwide. Researchers have developed a multiplex RT-PCR method to identify and quantify the prevalence and distribution of these viruses. The study reveals differences in the prevalence of different viruses during different seasons and competitive accumulation of viruses within melon plants. This study also provides the first report of SqVYV in Arizona and offers an efficient method for virus detection and quantification.
Article
Plant Sciences
Shaonpius Mondal, Laura Jenkins Hladky, William M. Wintermantel
Summary: Viruses transmitted by whiteflies pose a growing threat to cucurbit production worldwide. A study developed a multiplex RT-PCR method and TaqMan probes for the detection and quantification of these viruses in melon samples from California and Arizona. The study found differences in the prevalence and distribution of the viruses during different seasons and within different parts of the plants.
Article
Virology
Siyuan Liu, Wei Chen, Raphael Nyaruaba, Shunlong Wang, Cihan Yang, Qun Wu, Ying Liu, Puyu Liu, Fei Wang, Jingling Wang, Zhiming Yuan, Dingwei Sun, Han Xia
Summary: Specific, sensitive, and reliable RT-qPCR assays for the detection of OYAV and EBIV have been successfully developed in this study. These assays hold great promise for their potential application in clinical and field samples in the future.
Article
Fisheries
Jerome Delamare-Deboutteville, Suwimon Taengphu, Han Ming Gan, Pattanapon Kayansamruaj, Partho Pratim Debnath, Andrew Barnes, Shaun Wilkinson, Minami Kawasaki, Chadag Vishnumurthy Mohan, Saengchan Senapin, Ha Thanh Dong
Summary: This study successfully demonstrated a rapid diagnostic and genotyping method for tilapia lake virus using a combination of specific PCR assay and ONT-based amplicon sequencing, with high accuracy and operational feasibility. The approach is suitable for deployment in regional aquatic animal health diagnostic laboratories in low- and medium-income countries, allowing for fast identification and genotyping of emerging infectious pathogens from field samples within a single day.
JOURNAL OF FISH DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Hyuna Park, Wonjong Jung, Hyeongseok Jang, Kak Namkoong, Kwon-Young Choi
Summary: This study developed a simple and rapid thermolysis method that can bypass the need for viral RNA extraction and purification. The method integrates thermolysis, RT, and digital PCR into a single chip for large-scale viral analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Sri Rajiv Kumar Roy, Jidapa Yamkasem, Puntanat Tattiyapong, Win Surachetpong
Summary: The study indicates that small fish are more susceptible to TiLV infection compared to large fish, with severe pathological changes observed mainly in the liver, spleen, and intestine after infection.
Article
Environmental Sciences
Xiaodi Duan, Chiqian Zhang, Ian Struewing, Xiang Li, Joel Allen, Jingrang Lu
Summary: This study evaluated the efficacy of an early-warning system that uses cyanotoxin-encoding genes to predict harmful cyanobacterial blooms in Harsha Lake, Ohio. The results showed that the abundances and transcript levels of cyanotoxin-encoding genes were significantly correlated with total cyanotoxin concentrations and accurately predicted cyanotoxin production. This early-warning system can be a useful tool for lake managers in predicting and preventing harmful cyanobacterial blooms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Fisheries
Tao He, Yu-Zhou Zhang, Li-Hong Gao, Bo Miao, Ji-Shu Zheng, De-Cheng Pu, Qing-Qing Zhang, Wei-Wei Zeng, De-Shou Wang, Sheng-Qi Su, Song Zhu
Summary: As an emerging pathogen, tilapia lake virus (TiLV) has caused severe socio-economic impacts and remains a devastating factor in wild and farmed tilapia. Early diagnosis and timely reporting of TiLV are crucial. This study reports on a TiLV infection event in a tilapia farm, where naturally diseased tilapia with clinical signs and multiple tissue lesions were observed. TiLV was identified in liver, spleen, and kidney of the diseased tilapia. The isolated TiLV was found to have high sequence similarity to other isolates but placed in a unique cluster in phylogenetic analysis. Experimental infection with the isolated TiLV resulted in high mortality and similar clinical signs and tissue lesions in healthy Nile tilapia. Vacuolation, necrosis, inclusion bodies, and tissue lesions were observed in experimentally infected tilapia. Liver, spleen, and brain were identified as the main target tissues.
Article
Virology
Daiki Kanbayashi, Takako Kurata, Atsushi Kaida, Hideyuki Kubo, Seiji P. Yamamoto, Kazutaka Egawa, Yuki Hirai, Kazuma Okada, Yuko Kaida, Ryo Ikemori, Takahiro Yumisashi, Ayami Ito, Takeshi Saito, Yoshihiko Yamaji, Yuka Nishino, Ryosuke Omori, Haruyo Mori, Kazushi Motomura, Kazuyoshi Ikuta
Summary: This study analyzed samples from patients with rubella and found that virus shedding peaked 0-2 days after rash onset and decreased over time. The viral RNA load and presence of infectious rubella virus were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and virus isolation. These findings contribute to improved algorithms for rubella surveillance and diagnosis.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Fisheries
Nai-tong Yu, Wei-wei Zeng, Zhongguo Xiong, Zhi-xin Liu
Summary: This study successfully cloned TiLV ORF10 into the pcDNA3.1 vector as a vaccine candidate and showed that vaccination with pcDNA3.1-ORF10 can significantly upregulate the transcription levels of immune-related genes, reduce virus replication, and enhance protective effects against TiLV in tilapia.
AQUACULTURE REPORTS
(2022)
Article
Infectious Diseases
Jidapa Yamkasem, Sri Rajiv Kumar Roy, Matepiya Khemthong, Ian A. Gardner, Win Surachetpong
Summary: The use of pool testing for tilapia lake virus detection can efficiently and accurately estimate within-farm prevalence. Bayesian modelling can help to provide more accurate estimations of the prevalence of tilapia lake virus.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Fisheries
Pattarasuda Rawiwan, Matepiya Khemthong, Puntanat Tattiyapong, David Huchzermeyer, Win Surachetpong
Summary: The study found that using RNAlater (R) and deep-freezing at -20 degrees C are the best practices for maintaining TiLV genomic RNA, with minimal degradation over a period of 365 days.
Article
Fisheries
Jidapa Yamkasem, Chutchai Piewbang, Somporn Techangamsuwan, Felipe Pierezan, Esteban Soto, Win Surachetpong
Summary: The results indicate that ornamental Malawi cichlid can be infected with Tilapia lake virus (TiLV), leading to severe symptoms and mortality. The infected fish show various clinical signs and histopathological changes, suggesting that ornamental African cichlids could be potential carriers for TiLV.
Article
Virology
Pitchaporn Waiyamitra, Chutchai Piewbang, Somporn Techangamsuwan, Woei Chang Liew, Win Surachetpong
Summary: This study experimentally challenged Mozambique tilapia with a virulent strain of TiLV and found that high concentration TiLV caused skin issues, enteritis, and a mortality rate of 77.78% in this fish species. Severe histopathological changes were observed in the infected fish, confirming the susceptibility of Mozambique tilapia to TiLV infection.
Article
Fisheries
Jidapa Yamkasem, Puntanat Tattiyapong, Win Surachetpong
Summary: A new TaqMan probe-based quantitative PCR assay was developed for detecting Tilapia parvovirus, showing good repeatability and reproducibility with a detection limit of 10 copies/mu l. The assays had comparable sensitivity to a previously developed SYBR Green qPCR and were more sensitive than conventional PCR, without cross-reactivity with other viruses and bacteria. These assays offer high sensitivity and specificity in detecting low concentrations of TiPV DNA in infected tilapia samples, providing a valuable diagnostic tool for experimental and field samples.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Mikolaj Adamek, Alexander Rebl, Marek Matras, Christian Lodder, Sahar El Rahman, Magdalena Stachnik, Krzysztof Rakus, Julia Bauer, Alberto Falco, Verena Jung-Schroers, Chutchai Piewbang, Somporn Techangamsuwan, Win Surachetpong, Michal Reichert, Jens Tetens, Dieter Steinhagen
Summary: The emergence of viral diseases in fish, such as tilapia lake virus (TiLV), can cause high mortality and disrupt aquaculture production. A study found that different genetic strains of tilapia have varying levels of resistance to TiLV, with one strain exhibiting a survival rate of nearly 100%. The resistant strain showed lower viral loads and pro-inflammatory responses, suggesting that a stronger antiviral response and reduced pathology contribute to its resistance. These findings suggest the possibility of using TiLV-resistant strains as a cost-effective solution, but integrated measures of biosecurity, diagnostics, and vaccination should still be employed.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jidapa Yamkasem, Tharinthon Prasartset, Puntanat Tattiyapong, Kwanrawee Sirikanchana, Skorn Mongkolsuk, Esteban Soto, Win Surachetpong
Summary: This study investigated the persistence of Tilapia Lake Virus (TiLV) in different water samples, and found that the virus decreased in concentration over a period of 14 days, with a loss of infectivity observed within 3 to 7 days. These findings have implications for improving biosecurity practices in tilapia farms.
JOURNAL OF FISH DISEASES
(2022)
Article
Food Science & Technology
Pattarasuda Rawiwan, Yaoyao Peng, I. Gusta Putu Bayu Paramayuda, Siew Young Quek
Summary: With the increase in world population and global climate changes, ensuring adequate food supply is crucial. This review explores the potential of red seaweed proteins as an alternative protein source and discusses their applications in food and nutraceutical industries, environmental impact, affordability, and safety concerns. The findings suggest that red seaweed proteins have potential as sustainable alternatives to terrestrial proteins, but further research is needed to improve their extraction and enhance their functionalities for various applications.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Fisheries
Theerawut Phusantisampan, Jidapa Yamkasem, Puntanat Tattiyapong, Malinee Sriariyanun, Win Surachetpong
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Puntanat Tattiyapong, Sirikorn Kitiyodom, Teerapong Yata, Krittayapong Jantharadej, Mikolaj Adamek, Win Surachetpong
Summary: The newly developed chitosan nanoparticle TiLV immersion vaccine showed promising efficacy in laboratory and field trials, with improved relative percent survival and specific antibody response in vaccinated fish. The use of this nanovaccine is convenient and suitable for mass vaccination to protect fish from TiLV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Tharinthon Prasartset, Win Surachetpong
JOURNAL OF FISH DISEASES
(2023)
Review
Fisheries
Bei Wang, Kim D. Thompson, Eakapol Wangkahart, Jidapa Yamkasem, Melba G. Bondad-Reantaso, Puntanat Tattiyapong, Jianchang Jian, Win Surachetpong
Summary: In recent years, emerging and re-emerging diseases have had a significant economic impact on the tilapia industry. Without effective management and appropriate biosecurity measures, infectious diseases will continue to challenge the sustainability of global tilapia aquaculture. Enhancing immune response and improving husbandry management can help reduce the impact of diseases.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Neetu Shahi, Bhupendra Singh, Sumanta Kumar Mallik, Debajit Sarma, Win Surachetpong
Summary: In this study, we conducted an RNA-Seq analysis on the brains of mature male and female golden mahseer to explore the different gene expression profiles. A total of 1187 differentially expressed genes were identified, with 953 upregulated genes in the female brain and 234 upregulated genes in the male brain. Additionally, 1236 genes were downregulated in the female brain and 744 genes were downregulated in the male brain. Validation of the RNA-Seq results confirmed the differential expression of these genes, providing valuable insights for potential reproductive manipulation strategies in golden mahseer.
Article
Fisheries
Dorothea V. Megarani, Lowia Al-Hussinee, Kuttichantran Subramaniam, Preeyanan Sriwanayos, Kamonchai Imnoi, Bill Keleher, Pamela Nicholson, Win Surachetpong, Puntanat Tattiyapong, Paul Hick, Lori L. Gustafson, Thomas B. Waltzek
Summary: In this study, a TaqMan RT-qPCR assay for the detection of tilapia lake virus disease (TiLVD) was developed and validated. The assay showed high efficiency and sensitivity, and did not cross-react with other RNA viruses of fish. It proved to be useful for both research and diagnostic purposes.
DISEASES OF AQUATIC ORGANISMS
(2022)