Article
Fisheries
Shany Cohen, Omer Ilouz, Rivka Manor, Amir Sagi, Isam Khalaila
Summary: In this study, silencing of the VIH gene alone or in combination with the MIH gene successfully induced ovarian maturation and development in females of Macrobrachium rosenbergii, with more pronounced effects in mature individuals. Additionally, the dsRNA used to silence VIH also affected the expression of MIH, albeit with a delay compared to the dsRNA targeting both VIH and MIH simultaneously.
Article
Biochemistry & Molecular Biology
Baojuan Zeng, Yueru Ye, Jiajie Ma, Jiasheng Song
Summary: LmSug is a zinc-finger transcription factor sensitive to juvenile hormone, playing a vital role in female insect reproduction.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shipeng Han, Da Wang, Peng Song, Shuo Zhang, Yunzhuan He
Summary: This study cloned and characterized two genes (SfVg and SfVgR) of the fall armyworm Spodoptera frugiperda and evaluated their expression profiles. The results showed that both genes were highly expressed in female adults, with SfVg specifically expressed in the fat body and SfVgR highly expressed in the ovary. Depletion of either gene hindered oocyte maturation and ovarian development, leading to a significant decrease in fecundity. These findings reveal the importance of SfVg and SfVgR in the vitellogenesis of S. frugiperda, providing a theoretical foundation for the development of pollution-free pest control strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Genyu Mano, Shin G. Goto
Summary: This study found that the circadian clock controls the photoperiodic response by altering the expression of key elements in two independent endocrine pathways.
APPLIED ENTOMOLOGY AND ZOOLOGY
(2022)
Article
Endocrinology & Metabolism
Hao Yu, Bin Yang, Liuhao Wang, Sijia Wang, Kui Wang, Qisheng Song, Hongwei Zhang
Summary: This study investigated the role of neuropeptide hormone bursicon in the ovary development and reproduction of the hemipteran insect, whitefly. The results revealed that bursicon regulates vitellogenesis and reproduction by coordinating with the juvenile hormone signaling pathway.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agronomy
Chenglong Ye, Yalin Feng, Feifei Yu, Qiqi Jiao, Jiangen Wu, Zihong Ye, Pengjun Zhang, Chuanxin Sun, Kun Pang, Peiying Hao, Xiaoping Yu
Summary: The expression of NlATG3 in brown planthoppers varies among different growth stages, and injecting dsNlATG3 has a strong lethal effect on nymphs. RNAi silencing of NlATG3 significantly reduces the number of eggs laid per female and the hatchability of eggs.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agronomy
Yong-Kang Liu, Cai-Di Xu, Xu-Song Zheng, Lei Chao, Yan-Fei Zhou, Guo-Yong Li, Yan Wu, Xue-Lian Bai, Ting Zhou, Bin Tang, Hong-Xing Xu
Summary: Excessive zinc is toxic to plants and its effect on the herbivorous insect Nilaparvata lugens is still unclear. This study assessed the impact of zinc-stressed rice plants on N. lugens. The results showed that zinc treatment altered sugar content in rice, reduced fecundity and trehalose content in N. lugens, and impacted trehalose metabolism. These findings suggest that zinc-stressed plants may have negative effects on herbivorous insects.
Article
Physiology
Kui Kang, Youjun Cai, Lei Yue, Wenqing Zhang
Summary: In this study, the effects of nutritional conditions on growth and reproduction indicators of the brown planthopper (BPH) were tested using BPH as a model. The results showed that as the nutrient concentration decreased, the body development and oviposition of BPH were hindered. The expression levels of vitellogenin (Vg) genes were also significantly decreased in the lower nutrient concentration groups. These findings indicate that growth and reproduction in the BPH are regulated by, or correlated with, nutrient concentration.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Y. J. Zhang, L. Jiang, S. Ahamd, Y. Chen, J. Y. Zhang, D. Stanley, H. Miao, L. Q. Ge
Summary: The study found that OA2B2 plays a role in the BPH mating system, and silencing of OA2B2 leads to decreased survival following exposure to starvation, high temperature, and induced oxidative stress. Additionally, the research further confirms the role of OA2B2 in mediating BPH resistance to biotic and abiotic stressors.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Xin-Yang Liu, Ru Yan, Sun-Jie Chen, Jin-Li Zhang, Hai-Jun Xu
Summary: The migratory brown planthopper (BPH), Nilaparvata lugens, exhibits strong olfactory responses to major rice volatile compounds, and the Orco gene plays a crucial role in BPHs. By utilizing gene editing technology, researchers generated a NlOrco-knockout mutant of BPHs, which showed no response to rice volatile compounds and lost their preference for host plants. These findings have significant implications for BPH control applications and the identification of potential target genes or chemical compounds.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xiaobin Fan, Wenqing Zhang
Summary: This study investigates the function of fatty acyl-CoA reductases (FARs) in the brown planthopper and finds that NlFAR10 is highly expressed during embryogenesis. Loss of NlFAR10 affects embryogenesis and hatching rates, indicating a role in insect reproduction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Qi Wei, Xu-Hui Zhu, Pin-Jun Wan, Jia-Chun He, Wei-Xia Wang, Feng-Xiang Lai, Qiang Fu
Summary: The brown planthopper (BPH) is one of the most destructive pests in rice-growing regions of Asia. In this study, the researchers investigated the physiological properties of the SWI/SNF chromatin remodeling ATPase Brahma (BRM) in BPH. They found that NlBRM, the homolog of BRM in BPH, was abundantly expressed in the egg and ovary. Downregulation of NlBRM significantly affected female survival and decreased the number of oviposition marks and newly hatched BPH nymphs. The researchers concluded that NlBRM plays a crucial role in ovarian development and fecundity of BPH, potentially through regulating the vitellogenin gene.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Environmental Sciences
Zhe Zhao, Qiang Zhao, Haoyu Wang, Lan Wei, Siya Wang, Shilin Li, Dengyue Yuan, Zhijian Wang
Summary: This study aims to explore the molecular mechanism and regulatory factors behind vitellogenesis in Sichuan bream. The results show that energy redistribution during vitellogenesis is regulated through glycolysis, fatty acid biosynthesis, and the citrate cycle pathway. The liver and ovary exhibit different energy metabolism strategies, with the liver promoting energy metabolism and the ovary retaining nutrients. Several key factors involved in the metabolism and transport of important yolk nutrients have also been identified. The initiation and sustained increase in vitellogenesis are associated with the surge in serum estrogen levels and upregulation of the estrogen receptor, respectively. These findings provide valuable insights into the regulation of ovarian development in cultured fish.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sheraz Ahmad, Yu Chen, Jieyu Zhang, David Stanley, Qisheng Song, Linquan Ge
Summary: Mating causes physiological and behavioral changes in female insects, such as increased egg production and refractoriness to re-mating. Research on brown planthoppers showed that mating led to increased concentrations of octopamine (OA), cAMP, and enzyme activities in females, as well as changes in gene expression in the cAMP/PKA signal transduction pathway. Silencing genes involved in OA signaling resulted in decreased fecundity, body weights, and longevity in females, with an impact on ovarian development, egg maturation, and population growth.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Environmental Sciences
Yvonne Rericha, Lisa Truong, Connor Leong, Dunping Cao, Jennifer A. Field, Robyn L. Tanguay
Summary: Ubiquitous anthropogenic contaminants of concern, per- and polyfluoroalkyl substances (PFAS) are frequently detected in the environment and human populations around the world. Diet is a predominate route of human exposure, and PFAS are frequently measured in food. However, the current amount of data to support safety assessments of these alternatives is not yet sufficient. The present study investigated the effects of a 42-day dietary exposure to 1, 10, or 100 ng/g PFHxA in juvenile zebrafish. In conclusion, PFHxA exposure induced abnormal adult anxiety behaviors that persisted transgenerationally, and abnormal behavior was also observed in the F1 generation. This study highlights the need for further investigation of this and other alternative short-chain PFAS.
Article
Biotechnology & Applied Microbiology
Yibei Cheng, Yimin Li, Wenru Li, Yuanyuan Song, Rensen Zeng, Kai Lu
Article
Environmental Sciences
Yibei Cheng, Yimin Li, Wenru Li, Yuanyuan Song, Rensen Zeng, Kai Lu
Summary: The study demonstrates that inhibition of hepatocyte nuclear factor 4 confers imidacloprid resistance in brown planthopper by regulating cytochrome P450 and UDP-glycosyltransferase genes. Knockdown of HNF4 enhances resistance to imidacloprid in BPH, leading to up-regulation of cytochrome P450- and UGT-mediated metabolic detoxification pathways. Over-expression of UGT-1-7, UGT-2B10, and CYP6ER1 in the resistant strain indicates potential targets for pest management.
Article
Entomology
Kai Lu, Yi-Bei Cheng, Yi-Min Li, Wen-Ru Li, Yuan-Yuan Song, Ren-Sen Zeng, Zhong-Xiang Sun
Summary: This study reveals that KNRL negatively regulates Vn breakdown in brown planthoppers by suppressing the expression of hydrolase genes, influencing embryonic development. The findings suggest that KNRL controls Vn breakdown in embryos through the transcriptional inhibition of hydrolases.
Article
Biochemistry & Molecular Biology
Kai Lu, Yimin Li, Yibei Cheng, Wenru Li, Yuanyuan Song, Rensen Zeng, Zhongxiang Sun
Summary: Increased production of detoxification enzymes is a primary mechanism for insecticide resistance in crop pests. This study identified the NR2E nuclear receptor HR83 as critical for chlorpyrifos resistance in brown planthoppers, regulating the expression of detoxification genes. Knockdown of HR83 led to decreased resistance to chlorpyrifos and reduced expression of multiple detoxification genes, essential for the resistance of BPH to chlorpyrifos. This research suggests that regulation of detoxification genes may be an ancestral function of the NR2E nuclear receptor subfamily.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biology
Kai Lu, Yuanyuan Song, Rensen Zeng
Summary: The article discusses the role of insect P450-mediated detoxification in adaptation to xenobiotics and highlights recent progress in the molecular regulation of P450.
CURRENT OPINION IN INSECT SCIENCE
(2021)
Article
Environmental Sciences
Zhongxiang Sun, Rumeng Wang, Yifei Du, Binyuan Gao, Furong Gui, Kai Lu
Summary: Insecticide resistance is a major challenge in controlling agricultural pests. This study reveals that exposure to the herbicide butachlor can reduce susceptibility to insecticide chlorpyrifos in lepidopteran pests. The mechanism involves the olfactory recognition of herbicides by GOBP2, triggering insect hormone biosynthesis and leading to increased metabolic tolerance against insecticides.
ENVIRONMENTAL POLLUTION
(2021)
Article
Agriculture, Multidisciplinary
Kai Lu, Yibei Cheng, Yimin Li, Wenru Li, Rensen Zeng, Yuanyuan Song
Summary: The study revealed that pre-exposure to the flavonoid phytochemical flavone enhances larval tolerance to multiple synthetic insecticides and activates detoxification enzyme activities through the ROS/CncC pathway. The findings suggest a key role of the ROS/CncC pathway in driving detoxification gene expression responsible for insecticide tolerance after exposure to flavone.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Kai Lu, Yimin Li, Yibei Cheng, Wenru Li, Bixue Zeng, Chengzhen Gu, Rensen Zeng, Yuanyuan Song
Summary: The ingestion of xanthotoxin enhanced the tolerance of Spodoptera litura larvae to lambda-cyhalothrin by upregulating detoxification genes and transcription factors. This study also revealed the involvement of ROS/CncC pathway and 20E signaling pathway in the induction of metabolic detoxification upon exposure to xanthotoxin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Environmental Sciences
Kai Lu, Yimin Li, Tianxiang Xiao, Zhongxiang Sun
Summary: This study analyzed the expression patterns of CarE genes in chlorpyrifos-resistant strains, showing that CarE3, CarE17, and CarE19 were overexpressed. Knockdown of CarE17 or its upstream transcription factors Lim1β and C15 reduced the resistance of N. lugens to chlorpyrifos.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Kai Lu
Summary: The common cutworm, Spodoptera litura, has developed resistance to commonly used insecticides. Two P450 enzymes, CYP6AE43 and CYP6AE48, play a crucial role in detoxifying pyrethroids and promoting resistance development in S. litura.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Zhiming Yang, Tianxiang Xiao, Kai Lu
Summary: In this study, it was found that inhibiting the activity of UGT enzyme effectively increased the toxicity of chlorpyrifos to the chlorpyrifos-resistant strain of Nilaparvata lugens. The resistant strain showed higher content of UGT compared to the susceptible strain. Four UGT genes (UGT386H2, UGT386J2, UGT386N2, and UGT386P1) were significantly overexpressed in the resistant strain and could be induced by chlorpyrifos. Knockdown of UGT386H2 or UGT386P1 increased the susceptibility of the resistant strain to chlorpyrifos. These findings suggest that overexpression of these two UGT genes contributes to chlorpyrifos resistance in N. lugens.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qiong Yao, Linfa Quan, Siwei Wang, Dongxu Xing, Bingxu Chen, Kai Lu
Summary: This study investigated the effects of feeding with lambda-cyhalothrin-contaminated prey on the reproductive parameters of Eocanthecona furcellata. It was found that the insecticide affected the reproductive parameters of E. furcellata females, including prolonged premating and preoviposition durations, reduced number of egg batches and egg amount, disturbed ovarian development, and suppressed expression of reproductive related genes. Male bugs also showed reduced survival rate and altered carbohydrate metabolism parameters. These findings reveal the detrimental impacts of lambda-cyhalothrin-exposure on predators via prey intake.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhiming Yang, Tianxiang Xiao, Mengqing Deng, Wenxiu Wang, Haoxue Peng, Kai Lu
Summary: This study identified and analyzed 19 nuclear receptors (NRs) in the genome of Spodoptera litura larvae, and found that the transcriptional levels of most NRs were significantly induced in the midgut after exposure to flavone and xanthotoxin. Knockdown of four NRs (FTZ-F1, EcR, Dsf, and HR3) resulted in reduced larval tolerance to the phytochemicals. Additionally, many detoxification genes were downregulated by dsNR administrations, which may explain the high sensitivity of S. litura to phytochemicals. Molecular docking revealed that phytochemicals had high affinity to bind to NRs. This study provides insights into the mechanism of host adaptation in S. litura and supports the development of NR-targeted insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Tianxiang Xiao, Wenxiu Wang, Mengqing Deng, Zhiming Yang, Haoxue Peng, Zifan Huang, Zhongxiang Sun, Kai Lu
Summary: The induction of CYP321A subfamily members in Spodoptera litura for detoxification is mediated by the activation of CncC and MafK transcription factors, and knockdown of these transcription factors increases larval susceptibility to xenobiotics.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Zhiming Yang, Wenxiu Wang, Mengqing Deng, Haoxue Peng, Zifan Huang, Jianping Liu, Kai Lu
Summary: This study characterized twenty epsilon glutathione S-transferase genes (GSTes) in Spodoptera litura and analyzed their roles in phytochemical tolerance. Xanthotoxin, a phytochemical, induced the expression of most GSTe genes and formed stable bonds with six specific GSTes. Knockdown of these specific GSTe genes increased the larval susceptibility to xanthotoxin. Transcription factors CncC and MafK enhanced the expression of GSTe16, leading to increased xanthotoxin tolerance. The findings provide insight into the functions and transcriptional regulatory mechanisms of GSTes in the adaptation of S. litura to phytochemicals.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)