Article
Biochemistry & Molecular Biology
Shinta Wulansari, Sobroney Heng, Prattana Ketbot, Sirilak Baramee, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Ayaka Uke, Akihiko Kosugi, Chakrit Tachaapaikoon
Summary: A novel D-psicose 3-epimerase from a halophilic bacterium was characterized in this study. The enzyme was able to convert D-fructose into D-psicose, providing a potential application in food quality enhancement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jianwei Wang, Jiandong Sun, Hongqing Qi, Liang Wang, Jihui Wang, Cheng Li
Summary: The study successfully expressed d-Psicose 3-epimerase gene in Bacillus subtilis for the conversion of d-fructose to d-psicose. Immobilization of cells onto gel beads increased stability and recyclability, leading to higher d-psicose production compared to free cells. This approach shows promise for efficient synthesis of d-psicose.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
He Li, Yunjie Tu, Tao Tu, Qi Zhang
Summary: In this study, proteases were immobilized using a nanoflower-like zeolitic imidazole framework (nfZIF), which showed excellent performance in immobilizing proteases and resulted in protease@nfZIF composites with comparable or even higher activities than free proteases in solution. These composites demonstrated stability in high temperature and organic solvents, and retained almost full activity after multiple reaction cycles. This study highlights the great potential of nfZIF in protease immobilization, which could be of interest in various industrial applications.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Seyma Dadi, Nimet Temur, O. Tolga Gul, Vedat Yilmaz, Ismail Ocsoy
Summary: Organic-inorganic hybrid nanoflowers (NFs) composed of horseradish peroxidase (HRP) and copper II (Cu2+) were successfully synthesized using carbon nanotubes (CNTs) by in situ and post-modification methods. The catalytic activities of the synthesized HRP-NF@CNTs were examined, and it was found that the inclusion of CNTs greatly increased the catalytic activity and stability of the nanoflowers. The HRP-NF@CNTs were used for detecting hydrogen peroxide (H2O2) and glutathione (GSH) with colorimetric and spectrophotometric readouts.
Article
Biotechnology & Applied Microbiology
Jiaming Yang, Dexun Fan, Fengguang Zhao, Ying Lin, Suiping Zheng, Shuangyan Han
Summary: A novel D-allulose-3-epimerase (RpDAE) from Ruminiclostridium papyrosolvens was characterized in this study, which demonstrated efficient production of D-allulose under specific conditions. In addition, the immobilization of RpDAE within ZIF67 was successfully achieved, and the resulting enzyme composites maintained considerable catalytic activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Drew Jordahl, Zoe Armstrong, Qiaobin Li, Runxiang Gao, Wei Liu, Kelley Johnson, William Brown, Allison Scheiwiller, Li Feng, Angel Ugrinov, Haiyan Mao, Bingcan Chen, Mohiuddin Quadir, Hui Li, Yanxiong Pan, Zhongyu Yang
Summary: Metal-organic frameworks (MOFs) can immobilize enzymes through co-crystallization or diffusion, and biomineralization provides an effective method for enzyme immobilization under aqueous conditions. The stability of enzyme@MOF composites depends on the metal-ligand combinations and pH values. This study demonstrates the potential of expanding the library of metal-ligand selection for customized encapsulation of enzymes based on their stability, functionality, and optimal pH.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Xiao-Gang Yang, Ji-Rui Zhang, Xu-Ke Tian, Jian-Hua Qin, Xin-Ya Zhang, Lu-Fang Ma
Summary: Efficient enzyme immobilization is highly desirable for their recyclability and activity, and Zeolitic imidazolate framework-8 (ZIF-8) has been proved to be an effective platform for enzyme immobilization with its easy preparation and biocompatibility. However, the hydrophobic characteristic of ZIF-8 hinders its further development. This study developed a facile synthesis approach to immobilize pepsin (PEP) on ZIF-8 carrier using Ni2+ ions as an anchor, leading to remarkable catalytic activity for electrochemical oxygen evolution reaction (OER) with an ultralow overpotential.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Inorganic & Nuclear
Yingjie Du, Xiaotong Jia, Le Zhong, Yi Jiao, Zhijin Zhang, Ziyuan Wang, Yuxiao Feng, Muhammad Bilal, Jiandong Cui, Shiru Jia
Summary: This review presents the contemporary advances in the development of enzyme@MOF composites with different dimensionalities. The synthesis strategies, interaction between enzymes and MOFs, and potential applications are discussed. This work provides helpful insights into the fundamental research and applications of enzyme@MOF composites.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Physical
Zixin Zhou, Hao Chao, Wenting He, Ping Su, Jiayi Song, Yi Yang
Summary: In this study, a facile strategy was presented to address the limited substrate accessibility and unfavorable interfacial interactions in metal-organic framework (MOF)-enzyme complexes. By grafting short-chain polyacrylic acid (PAA) onto the enzyme surface, the microenvironment of the enzyme inside the MOF was improved, leading to enhanced catalytic activity and resistance to extreme conditions.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Dali Wei, Mingwei Li, Fengxiang Ai, Kun Wang, Nuanfei Zhu, Ying Wang, Daqiang Yin, Zhen Zhang
Summary: The cooperation of biocatalysis and chemocatalysis in a catalytic cascade reaction has been widely studied. However, practical applications are hindered by enzyme fragility, poor compatibility between carriers and enzymes, and limited catalytic efficiency. In this study, a biomimetic cascade nanoreactor (GOx@COFs@Os) was developed, integrating glucose oxidase (GOx) and Os nanozyme with covalent organic framework (COF) capsules. The GOx@COFs@Os capsule provided a spacious microenvironment to retain GOx activity and protect it from incompatible environments. The COF capsule also improved substrate affinity and mass transfer efficiency, resulting in significantly improved catalytic efficiency compared to the free cascade system. Moreover, the biomimetic cascade capsule was successfully used for glucose monitoring, glutathione sensing, and bisphenol S detection. This strategy provides a new avenue for improving biocatalytic cascade performance and expanding its applications in various fields.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Hyeryeong Lee, Eun Mi Lee, Stacy Simai Reginald, In Seop Chang
Summary: This study compared the effects of different gold-binding peptides fused to enzymes on electrocatalytic activity, bioactivity, and material-binding behaviors, demonstrating the vital role of GBP type in the design, construction, and optimization of enzyme-modified electrodes for efficient interfacial DET in DET-based enzyme-electrode systems.
Article
Chemistry, Analytical
Yi Feng, Yuting Zhao, Jun Ge
Summary: Metal-organic frameworks (MOFs) are promising carriers for enzymes and other biomolecules due to their stability, selectivity, and controllability. However, enzyme-MOF complexes often result in decreased enzyme activity and substrate affinity. In this study, enzyme-MOF composites were synthesized using ZIF-8, with adjusted sizes leading to enhanced substrate affinity and enzyme activity, showing potential for efficient biosensors in biomedicine.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Diego Carballares, Roberto Fernandez-Lafuente, Javier Rocha-Martin
Summary: This paper presents different methodologies for the immobilization of D-amino acid oxidase (DAAO). The results suggest that immobilization on glutaraldehyde support at pH 7 provides the best stability and activity for the enzyme.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Hongxia Li, Qi Lu, Junxiao Shi, Xiaoguang Zhang, Peng Sun, Xu Yan, Geyu Lu
Summary: This study demonstrates an enzyme-engineered assembly strategy for the fusion of acetylcholinesterase into Zn-based metal-organic frameworks, which maintains the biological state of the enzyme and provides stability and improved mass transfer. Additionally, a robust biosensor is constructed for monitoring the degradation of organophosphorus pesticides in water.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biophysics
Yong Liu, Xinxin Shao, Deqiang Kong, Guangquan Li, Quanshun Li
Summary: Thermophilic lipase QLM from Alcaligenes sp. was successfully immobilized in Cu-3(PO4)2-based inorganic hybrid nanoflower through biomimetic mineralization. The immobilized enzyme exhibited enhanced stability and reusability compared with the free enzyme, making it a promising candidate for future biocatalytic applications. The optimization of temperature and pH conditions, as well as the protective effect of the nanoflower shell, were critical factors contributing to the favorable properties of the immobilized enzyme.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
