期刊
CATALYSTS
卷 8, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/catal8100460
关键词
biocatalysis; Combi-CLEAs; cascade reactions; immobilization
资金
- Guangxi Natural Science Foundation [2016GXNSFCA380011]
- Seagull Plan from Qinzhou city
Enzymes are efficient biocatalysts providing an important tool in many industrial biocatalytic processes. Currently, the immobilized enzymes prepared by the cross-linked enzyme aggregates (CLEAs) have drawn much attention due to their simple preparation and high catalytic efficiency. Combined cross-linked enzyme aggregates (combi-CLEAs) including multiple enzymes have significant advantages for practical applications. In this review, the conditions or factors for the preparation of combi-CLEAs such as the proportion of enzymes, the type of cross-linker, and coupling temperature were discussed based on the reaction mechanism. The recent applications of combi-CLEAs were also reviewed.
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