Article
Forestry
Gaoman Linghu, Zhaoyou Yu, Meng Li, Anqi Wang, Yongxiang Kang
Summary: Davidia involucrata is a rare and endangered plant species with low reproductive efficiency. Somatic embryogenesis, a biotechnological method, offers a solution to improve the reproductive efficiency of D. involucrata. RNA sequencing analysis revealed that certain hormone-related gene families were upregulated in embryogenic callus compared to non-embryogenic callus, indicating their crucial roles in cell proliferation, differentiation, and totipotency in D. involucrata. These findings provide new insights into improving the induction, culture conditions, and genetic transformation efficiency of somatic embryogenesis in D. involucrata.
Article
Plant Sciences
Fenni Lv, Peng Wang, Enliang Zhang, Lingling Ma, Lulu Gao, Rutong Yang, Qing Wang, Ya Li
Summary: Efficient and stable transformation systems were developed from embryogenic calli in five genotypes of Catalpa bungei, with a positive frequency up to 92.31%. Transgenic lines expressing Cry2A and Cry9Aa-like genes showed significantly higher resistance to Omphisa plagialis, indicating great potential for future pest management in changing climatic conditions. This transformation system could also be a valuable tool for gene function analysis and genetic improvement of forest trees.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Forestry
Yan An, Hao Dong, Wenna Zhao, Hailong Shen, Ling Yang, Shujuan Li
Summary: In this study, a novel genetic transformation method for efficient transformation of Fraxinus mandshurica embryonic callus was investigated. The optimal conditions for transformation efficiency were determined, including antibiotic concentration, infection solution concentration, co-culture time, and somatic embryo maturation. These results are of great significance for gene function verification and future genetic improvement of Fraxinus mandshurica.
Article
Plant Sciences
Dipti Tonk, Abdul Mujib, Mehpara Maqsood, Mir Khusrau, Ali Alsughayyir, Yaser Hassan Dewir
Summary: This study investigated the effect of Fusarium oxysporum on the yields of vinblastine and vincristine alkaloids in Catharanthus roseus embryos. The addition of F. oxysporum extract improved the growth of callus biomass and enhanced the accumulation of vinblastine and vincristine in the tissues. Low doses of the Fusarium treatment resulted in high germination rates and longer shoot and root lengths of somatic embryos. The yields of vinblastine and vincristine were measured, with the highest and increased yields observed in F. oxysporum-amended tissues.
Article
Biochemistry & Molecular Biology
Yaping Wang, Hao Wang, Wenquan Bao, Mingming Sui, Yu'e Bai
Summary: Picea mongolica, a rare tree species in China, plays a significant role in combating desertification and improving the harsh ecological environment. However, due to various challenges, such as low natural regeneration rate and high mortality, Picea mongolica is facing extinction. This study aimed to compare the morphological, histological, physiological, and transcriptional differences between embryogenic callus (EC) and non-embryonic callus (NEC) in Picea mongolica, providing insights into the key genes involved in EC formation.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Horticulture
Chang Ho Ahn, Jung Yeon Han, Hyeong Soo Park, Hyun Won Yoon, Jung Won Shin, Jeong Min Seo, Hana Lee, Yeoung Ryul Kim, Saeng Geul Baek, Jae Ik Nam, Jung Min Kim, Yong Eui Choi
Summary: In this study, transcriptomic analysis identified three genes (TkBBM, TkWOX, and TkSERK) that are involved in somatic embryogenesis of Thuja koraiensis Nakai. These genes were highly expressed during somatic embryogenesis and may play important roles in this process. The findings of this study can be used as a reference for comparative transcriptome analysis in related species and further functional genomics studies in conifers.
Article
Biochemistry & Molecular Biology
Fang Gao, Chunxue Peng, Yue Zhang, Hao Wang, Hailong Shen, Ling Yang
Summary: This study reveals that intracellular glutathione might be the key regulator mediating the difference in proliferative capacity between different cell lines in somatic embryogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Shuting Zhang, Chen Zhu, Xueying Zhang, Mengyu Liu, Xiaodong Xue, Chunwang Lai, Xu Xuhan, Yukun Chen, Zihao Zhang, Zhongxiong Lai, Yuling Lin
Summary: This study used high-resolution single-cell RNA sequencing analysis to investigate the cellular changes and differentiation trajectories during longan somatic embryogenesis. The EC cells were divided into different clusters, including the epidermal cell cluster where overexpression of a marker gene inhibited triacylglycerol hydrolysis. The stability of autophagy and the role of ETHYLENE RESPONSIVE FACTOR 6 as a heat-sensitive regulator were also uncovered. These findings provide new spatiotemporal insights into cell division and differentiation during longan somatic embryogenesis.
Article
Multidisciplinary Sciences
Fang Gao, Xi Cao, Caiyun Qin, Shigang Chen, Jufeng Cai, Changbin Sun, Lisheng Kong, Jing Tao
Summary: Embryogenic tissue (ET) is crucial for genetic modification and plant re-generation. This study induced ET from mature zygotic embryos in blue spruce and investigated the effects of different factors on ET proliferation and somatic embryo (SE) maturation. The specific concentrations of 2,4-D and BA during the ET proliferation stage significantly affected both ET growth and the quality of ET for SE maturation. Developing genotype-specific protocols is necessary for efficient production of mature SEs or somatic plants in blue spruce.
SCIENTIFIC REPORTS
(2023)
Article
Horticulture
Amal F. M. Zein El Din, Ola H. Abd Elbar, Saleh M. Al Turki, Khaled M. A. Ramadan, Hossam S. El-Beltagi, Hemmat A. Ibrahim, Ezzeldin G. Gadalla, Ibrahim M. Shams El-Din, Ibrahim S. Ibrahim, Reham Farag, Mohamed F. M. Ibrahim, Mona S. Abd El-Aal, Ahmed Abou El-Yazied, Mohamed M. El-Mogy, Mina S. F. Samaan, Yasmin M. R. Abdellatif
Summary: The study aimed to investigate morpho-anatomical aspects, metabolic changes of proteins, antioxidant substances, and phenolic compounds in embryogenic callus (EC) and degenerative embryogenic callus (DEC). The ability to form somatic embryos was associated with the softness of the EC and higher concentrations of proteins, GSH, and ASC compared to DEC. The DEC showed a loss of embryogenic competence and had accumulations of oxidized ascorbic acid, H2O2, phenolic compounds, and naringenin.
Article
Plant Sciences
Aigul Amirova, Symbat Dossymbetova, Yeldana Rysbayeva, Bakdaulet Usenbekov, Arman Tolegen, Alibek Ydyrys
Summary: The aim of this study was to investigate the effect of plant growth regulators on callus induction and in vitro morphogenesis of Paulownia tomentosa and develop an efficient plant regeneration protocol. It was found that the addition of TDZ and NAA stimulated somatic embryogenesis, while the combination of BAP and NAA was optimal for callus induction and multiple plant regeneration. The regenerative capacity of P. tomentosa varied depending on the type of explant, occurring through both callus formation and direct regeneration. Overall, this study successfully developed a reproducible protocol for embryogenic callus formation and multiple shoot induction in vitro, leading to an increased frequency of plant regeneration.
Article
Plant Sciences
Jianhua Yue, Yan Dong, Songhu Liu, Yanan Jia, Chaoxin Li, Zhiyong Wang, Shoufu Gong
Summary: This study investigated the morphological, physiological, and molecular characteristics of the acquisition of embryogenic ability in Agapanthus praecox (A. praecox) through large-scale, quantitative proteomic and metabolomic analyses. Integrated analyses suggested that carbohydrate and energy metabolism, chromatin accessibility and DNA methylation, reactive oxygen species responses and resistance, and plant hormonal signaling potentially regulated callus differentiation direction. It was found that carbon source combination and plant hormone regulation significantly affected the acquisition of embryogenic ability. Overall, this study provided valuable insights into the mechanisms associated with somatic embryogenesis and organogenesis in A. praecox.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ying Wang, Hui-Liang Li, Yong-Kai Zhou, Dong Guo, Jia-Hong Zhu, Shi-Qing Peng
Summary: The study analyzed the transcriptomes of rubber tree late SE and identified differentially expressed genes involved in hormone signaling pathways, phenylpropanoid biosynthesis, and starch metabolism. Molecular markers for rubber tree late SE include genes related to hormone signaling and transcription factors, as well as genes related to histone modification.
Article
Multidisciplinary Sciences
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Mathew Ngugi, Richard Oduor, Wilton Mwema Mbinda
Summary: This study evaluated factors influencing protoplast isolation, callus induction, and plant regeneration in cassava, and developed a simple and highly reproducible protocol.
Article
Forestry
Xiaofeng Zeng, Xiao Wang, Yanling Zeng, Jinbo Hou, Zhiming Liu
Summary: In this study, callus induction of Catalpa bungei C. A. Mey was successfully achieved using different media and hormone concentrations. Metabolomic analysis identified the major flavonoid constituents in the callus tissue. The addition of salicylic acid and yeast extract increased the total flavonoid content in the callus, with only yeast extract promoting diosmin formation. Optimal medium formulations were determined to promote callus formation and increase the content of geraniol glucoside.