期刊
FRONTIERS IN PLANT SCIENCE
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2018.01527
关键词
maize; doubled haploids; maternal haploid inducers; marker-assisted selection; phenotyping
资金
- Bill and Melinda Gates foundation (BMGF) [OPP1028335]
- Stress tolerant Maize for Africa (STMA) project [OPP1134248]
- BMGF
- USAID
- CGIAR Research Program on Maize (MAIZE)
- MasAgro project - Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA), Govt. of Mexico
- Limagrain
- Government of Australia
- Government of Belgium
- Government of Canada
- Government of China
- Government of France
- Government of India
- Government of Japan
- Government of Korea
- Government of Mexico
- Government of Netherlands
- Government of New Zealand
- Government of Norway
- Government of Sweden
- Government of Switzerland
- Government of United Kingdom
- Government of United States
- World Bank
- Bill and Melinda Gates Foundation [OPP1028335] Funding Source: Bill and Melinda Gates Foundation
For efficient production of doubled haploid (DH) lines in maize, maternal haploid inducer lines with high haploid induction rate (HIR) and good adaptation to the target environments is an important requirement. In this study, we present second-generation Tropically Adapted Inducer Lines (2GTAILs), developed using marker assisted selection (MAS) for qhir1, a QTL with a significant positive effect on HIR from the crosses between elite tropical maize inbreds and first generation Tropically Adapted Inducers Lines (TAILs). Evaluation of 2GTAILs for HIR and agronomic performance in the tropical and subtropical environments indicated superior performance of 2GTAILs over the TAILs for both HIR and agronomic performance, including plant vigor, delayed flowering, grain yield, and resistance to ear rots. One of the new inducers 2GTAIL006 showed an average HIR of 13.1% which is 48.9% higher than the average HIR of the TAILs. Several other 2GTAILs also showed higher HIR compared to the TAILs. While employing MAS for qhir1 QTL, we observed significant influence of the non-inducer parent on the positive effect of qhir1 QTL on HIR. The non-inducer parents that resulted in highest mean HIR in the early generation qhir1+ families also gave rise to highest numbers of candidate inducers, some of which showed transgressive segregation for HIR. The mean HIR of early generation qhir1+ families involving different non-inducer parents can potentially indicate recipient non-inducer parents that can result in progenies with high HIR. Our study also indicated that the HIR associated traits (endosperm abortion rate, embryo abortion rate, and proportion of haploid plants among the inducer plants) can be used to differentiate inducers vs. non-inducers but are not suitable for differentiating inducers with varying levels of haploid induction rates. We propose here an efficient methodology for developing haploid inducer lines combining MAS for qhir1 with HIR associated traits.
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