Article
Immunology
Alvaro Lopez-Valinas, Marta Valle, Marta Perez, Ayub Darji, Chiara Chiapponi, Llilianne Ganges, Joaquim Segales, Jose I. Nunez
Summary: Influenza A viruses (IAVs) are constantly evolving, posing a threat to animal and human health. Vaccines can help reduce the spread of swine influenza viruses (SIAV) in farms, but they do not provide complete protection and may potentially favor viral evolution. A study on vaccinated and nonvaccinated pigs challenged with H1N2 SIAV showed that vaccinated animals had a higher proportion of virus mutations, indicating the potential for vaccine-driven evolution.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Virology
Cynthia Y. Tang, Karen Segovia, Jane A. McElroy, Tao Li, Minhui Guan, Xiaojian Zhang, Shamita Misra, Jun Hang, Xiu-Feng Wan
Summary: The majority of IBV influenza strains in Missouri during the 2019-2020 season contained a three-amino acid deletion on the HA protein and were antigenically distant from the vaccine strain. Cell-adapted mutations were mainly found in the RNA polymerase. The study suggests that hCK is an effective platform for IBV isolation and culture-adapted mutations may influence virus studies and vaccine development.
Article
Multidisciplinary Sciences
Zilong Zhang, Shenwei Li, Xiaolin Zhu, Jian Hou, Hong Zhang, Baihui Zhao, Zhengan Tian
Summary: Research collected respiratory specimens of passengers with influenza-like illness at the Shanghai port during 2016/2017, identifying multiple subclades and amino acid substitutions of influenza A(H3N2) viruses. The results showed imported A(H3N2) strains were not a good match for the vaccine strain.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Sergio Ortega-del Campo, Luis Diaz-Martinez, Patricia Moreno, Esther Garcia-Rosado, M. Carmen Alonso, Julia Bejar, Ana Grande-Perez
Summary: NNV is a neurotropic virus that causes viral nervous necrosis disease in fish. It has a bisegmented (+) ssRNA genome and the variability of its genome may be associated with its virulence.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Deena R. Blumenkrantz, Thomas Mehoke, Kathryn Shaw-Saliba, Harrison Powell, Nicholas Wohlgemuth, Hsuan Liu, Elizabeth Macias, Jared Evans, Mitra Lewis, Rebecca Medina, Justin Hardick, Lauren M. Sauer, Andrea Dugas, Anna DuVal, Andrew P. Lane, Charlotte Gaydos, Richard Rothman, Peter Thielen, Andrew Pekosz
Summary: The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade, which dominated subsequent seasons. Research found that a virus with the novel NAg+F2P genotype replicated less well in human nasal epithelial cell cultures, and viruses with this genotype were associated with increased cough and shortness of breath in infected patients based on retrospective analyses of clinical data.
Article
Virology
Deena R. Blumenkrantz, Thomas Mehoke, Kathryn Shaw-Saliba, Harrison Powell, Nicholas Wohlgemuth, Hsuan Liu, Elizabeth Macias, Jared Evans, Mitra Lewis, Rebecca Medina, Justin Hardick, Lauren M. Sauer, Andrea Dugas, Anna DuVal, Andrew P. Lane, Charlotte Gaydos, Richard Rothman, Peter Thielen, Andrew Pekosz
Summary: The 2014-15 influenza season witnessed the emergence of a new variant affecting the H3N2 virus, which dominated in subsequent flu seasons. This variant, associated with increased cough and shortness of breath in infected patients, had a presence of specific mutations in the NA and PB1-F2 genes.
Article
Virology
Andres F. Ospina-Jimenez, Arlen P. Gomez, Maria A. Rincon-Monroy, Lucia Ortiz, Daniel R. Perez, Mario Pena, Gloria Ramirez-Nieto
Summary: This study reveals for the first time the existence of antigenic diversity of FLUAV in Colombia and emphasizes the impact of spatial and temporal factors on this diversity. The results are significant for monitoring the variability and antigenic characteristics of swine influenza virus under natural conditions.
Article
Infectious Diseases
Victor Alberto Hernandez-Hernandez, Anjarath Lorena Higuera-Iglesias, Gabriel Palma-Cortes, Daniela Tapia-Trejo, Santiago Avila-Rios, Ruben Roberto Gonzalez-Fernandez, Luis Angel Perez-Moreno, Joaquin Alejandro Zuniga-Ramos, Cristobal Guadarrama-Perez, Jose Luis Sandoval-Gutierrez, Carlos Cabello-Gutierrez
Summary: This study evaluated the vaccine effectiveness (VE) and mutations of influenza viruses in the Mexican population in early 2018. The results showed that the VE for the influenza A(H3N2) subtype was the lowest, possibly due to the co-circulation of multiple viral subtypes and subclades.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Nuo Xu, Yuwei Wu, Yulian Chen, Yue Li, Yuncong Yin, Sujuan Chen, Huiguang Wu, Tao Qin, Daxin Peng, Xiufan Liu
Summary: The effects of glycosylation site modification on hemagglutinin (HA) on the biological characteristics of the H5N6 subtype avian influenza virus (AIV) were investigated. H5N6 AIVs containing a 129-glycosylation site on HA were found to be prevalent strains in China, showing increased thermostability, decreased pH stability, and attenuated pathogenicity and contact transmission in chickens. Furthermore, these H5N6 AIVs escaped the neutralization activity of serum antibodies.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Yuyun Li, Dongming Wang, Lili Zhi, Yunmei Zhu, Lan Qiao, Yan Zhu, Xin Hu, Qian Wang, Yuan Cao, Yan Gao, Yousheng Chen, Zeng Zhang, Fangjie Bi, Guangxing Yan
Summary: This study described respiratory tract infections occurred in children with allergic asthma on allergen immunotherapy during an influenza season. The results showed that children on AIT with maintenance doses had fewer symptoms and recovered quickly, suggesting that AIT with dose maintenance may enhance disease resistance of the body.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Gregory Queromes, Emilie Frobert, Elena Burtseva, Anca Draganescu, Paravaiz A. Koul, Andrey Komissarov, V. Alberto Laguna-Torres, Jason Leblanc, F-Xavier Lopez-Labrador, Snezana Medic, Alla Mironenko, Nancy A. Otieno, Guillermo M. Ruiz-Palacios, M. Tanriover, Laurence Josset, Bruno Lina
Summary: The Global Influenza Hospital Surveillance Network (GIHSN) confirms its ability to provide valuable information on influenza infection dynamics in hospital settings through standardized data collection and analysis.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Virology
Hechao Zhu, Xiangmin Li, Huanchun Chen, Ping Qian
Summary: A/swine/Heilongjiang/GN/2020 (EA-H1N1) virus was isolated from pregnant sows with miscarriage and respiratory disease in Heilongjiang province, China. The virus exhibited high pathogenicity and could cause severe respiratory tract damage in mice, with the potential of systemic spread.
Article
Immunology
Ying Wang, Xiao Xiao, Shipeng Chen, Chenjun Huang, Jun Zhou, Erhei Dai, Ya Li, Lijuan Liu, Xianzhang Huang, Zhiyuan Gao, Chuanyong Wu, Meng Fang, Chunfang Gao
Summary: This study aimed to explore the molecular mechanism of the coexistence of hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (HBsAb) serological pattern via intensive characterization of HBV s gene in both chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC) patients. Results show significant differences between HBsAg+/HBsAb+ and HBsAg+/HBsAb- in both CHB and HCC patients in terms of missense mutations, stop codon mutation changes, clustering, and random forest models. Furthermore, the study indicates that point mutations and quasispecies diversities of HBV s gene could alter the major hydrophilic region (MHR) antigenicity and CTL Se immunogenicity, contributing to different features of concurrent HBsAg+/HBsAb+ in HCC and CHB.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Infectious Diseases
Jing Xia, Yu-Wen Luo, Meng-Yi Dong, Yong-Xin Li, An-Dong Wang, Nian-Ling Li, Yu-Xi Shen, Shu-Yun Li, Min Cui, Xin-Feng Han, Song-Cheng Yu, Min Li, Yong Huang
Summary: This study identified two mutation sites (A168N and D201G) in H9N2 AIV that significantly affected antigenicity, without altering the growth kinetics of the virus. The D201G substitution not only changed antigenicity, but also caused immune escape from the parental virus. These mutation sites should be monitored during outbreaks.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Xiao-Guang Li, Jing Chen, Wei Wang, Fei Lin, Lu Li, Jing-Jin Liang, Zhong-Hua Deng, Bi-Ying Zhang, Ying Jia, Yuan-Bo Su, Yong-Feng Kang, Juan Du, Ya-Qiong Liu, Jie Xu, Qing-Bin Lu
Summary: This study conducted in northern China investigated the clinical efficacy of oseltamivir for patients with influenza virus infection. The results showed that oseltamivir therapy reduced the number of clinic visits for influenza-like illness patients, but did not have a significant effect on hospitalization frequency or the duration of fever and cough.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Infectious Diseases
Els Duysburgh, Laure Mortgat, Cyril Barbezange, Katelijne Dierick, Natalie Fischer, Leo Heyndrickx, Veronik Hutse, Isabelle Thomas, Steven Van Gucht, Bea Vuylsteke, Kevin K. Arien, Isabelle Desombere
LANCET INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Lorenzo Subissi, Nathalie Bossuyt, Marijke Reynders, Michele Gerard, Nicolas Dauby, Patrick Lacor, Siel Daelemans, Benedicte Lissoir, Xavier Holemans, Koen Magerman, Door Jouck, Marc Bourgeois, Benedicte Delaere, Sophie Quolin, Steven Van Gucht, Isabelle Thomas, Cyril Barbezange
Summary: The study in Belgium found that non-influenza respiratory viruses (NIRV) contributed significantly to severe acute respiratory infections (SARI), with a high positivity rate among children, particularly those under 15 years old. The incidence rates of NIRV-associated SARI were higher than influenza, especially in children under 5 years old. Co-infections with multiple NIRV and other respiratory viruses further contributed to the burden of SARI, highlighting the importance of early testing for NIRV in clinical management, especially for young children.
Article
Infectious Diseases
Dung Nguyen, Peter Simmonds, Maurice Steenhuis, Elise Wouters, Daniel Desmecht, Mutien Garigliany, Marta Romano, Cyril Barbezange, Piet Maes, Bram Van Holm, Joaquin Mendoza, Salvador Oyonarte, Anders Fomsgaard, Ria Lassauniere, Eva Zusinaite, Katarina Resman Rus, Tatjana Avsic-Zupanc, Johan H. J. Reimerink, Fiona Brouwer, Marieke Hoogerwerf, Chantal B. E. M. Reusken, Gunnveig Grodeland, Sophie Le Cam, Pierre Gallian, Abdennour Amroun, Nadege Brisbarre, Christophe Martinaud, Isabelle Leparc Goffart, Hubert Schrezenmeier, Hendrik B. Feys, C. Ellen van der Schoot, Heli Harvala
Summary: The research compared the performance of SARS-CoV-2 neutralising antibody testing among 12 European laboratories involved in convalescent plasma trials. It was found that there were significant differences in titres between laboratories during blind-testing, but calibration of titres reduced this variability. Harmonisation of neutralising antibody quantification is a crucial step towards determining protective and therapeutic levels of neutralising antibodies.
Letter
Medicine, Research & Experimental
Xavier Montagutelli, Sylvie van der Werf, Felix A. Rey, Etienne Simon-Loriere
Summary: SARS-CoV-2 Omicron variant has substitutions in the receptor binding domain of the spike protein, indicating its potential to infect rodents. The existence of wild animal reservoirs could facilitate the emergence of new variants with the risk of transmission to humans, and therefore should be closely monitored.
EMBO MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Meetali Singh, Maxime Chazal, Piergiuseppe Quarato, Loan Bourdon, Christophe Malabat, Thomas Vallet, Marco Vignuzzi, Sylvie van der Werf, Sylvie Behillil, Flora Donati, Nathalie Sauvonnet, Giulia Nigro, Maryline Bourgine, Nolwenn Jouvenet, Germano Cecere
Summary: A virus-derived miRNA produced during SARS-CoV-2 infection can target interferon-stimulated genes and suppress their expression, potentially aiding the virus in evading the host immune response. Detection of these viral miRNA isoforms in COVID-19 patients' samples suggests a mechanism by which the virus hijacks the host miRNA machinery.
Article
Biochemistry & Molecular Biology
Andrea Di Gioacchino, Rachel Legendre, Yannis Rahou, Valerie Najburg, Pierre Charneau, Benjamin D. Greenbaum, Frederic Tangy, Sylvie van der Werf, Simona Cocco, Anastassia Komarova
Summary: It is crucial to study the production of sgRNA in the vast amount of NGS data of SARS-CoV-2, and a user-friendly bioinformatic tool called sgDI-tector is introduced to identify and quantify these sgRNAs. Researchers successfully detected the nested set of sgRNAs in SARS-CoV-2 using this tool, and compared their production level with other types of viral RNA products.
Article
Immunology
Nathalie Vanderheijden, Annelies Stevaert, Jiexiong Xie, Xiaolei Ren, Cyril Barbezange, Sam Noppen, Isabelle Desombere, Bruno Verhasselt, Peter Geldhof, Nick Vereecke, Veerle Stroobants, Dayoung Oh, Merijn Vanhee, Lieve M. J. Naesens, Hans J. Nauwynck
Summary: Broadly protective therapeutic antibodies are urgently needed to combat SARS-CoV-2 variants. Researchers have found that antibodies formed during coronavirus infection can target multiple coronaviruses. These antibodies can bind to both alpha- and betacoronaviruses, but their neutralizing activity against SARS-CoV-2 is weak. However, they may still play a role in influencing the severity of coronavirus infection and disease through Fc-mediated effector functions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Camille Bonnet, Shirley Masse, Hayat Benamar, Ana-Maria Vilcu, Morgane Swital, Thomas Hanslik, Sylvie van der Werf, Xavier Duval, Fabrice Carrat, Alessandra Falchi, Thierry Blanchon
Summary: The study compared the evolution of viral load between patients infected with the Alpha variant and those infected with historical SARS-CoV-2 strains, finding no significant difference in viral load evolution in saliva samples when controlling for the time interval between symptom onset and sampling.
Article
Virology
Victoria Leclercq, Nayema van den Houte, Lydia Gisle, Inge Roukaerts, Cyril Barbezange, Isabelle Desombere, Els Duysburgh, Johan Van der Heyden
Summary: This study examined the prevalence of anti-SARS-CoV-2 antibodies and the factors associated with their presence in the Belgian adult population. The results showed a significant increase in antibody prevalence after the onset of the vaccination campaign, while the prevalence remained stable among the unvaccinated individuals.
Article
Multidisciplinary Sciences
Yu Wu, Nassim Mahtal, Elea Paillares, Lea Swistak, Sara Sagadiev, Mridu Acharya, Caroline Demeret, Sylvie Van der Werf, Florence Guivel-Benhassine, Olivier Schwartz, Serena Petracchini, Amel Mettouchi, Lucie Caramelle, Pierre Couvineau, Robert Thai, Peggy Barbe, Mathilde Keck, Priscille Brodin, Arnaud Machelart, Valentin Sencio, Francois Trottein, Martin Sachse, Gaetan Chicanne, Bernard Payrastre, Florian Ville, Victor Kreis, Michel-Robert Popoff, Ludger Johannes, Jean-Christophe Cintrat, Julien Barbier, Daniel Gillet, Emmanuel Lemichez
Summary: The compound C910 has been identified to disrupt toxin transport pathways, protecting cells against various bacterial toxins and reducing influenza and SARS-CoV-2 viral infections.
Article
Infectious Diseases
Els Tobback, Sophie Degroote, Sabine Buysse, Liesbeth Delesie, Lucas Van Dooren, Sophie Vanherrewege, Cyril Barbezange, Veronik Hutse, Marta Romano, Isabelle Thomas, Elizaveta Padalko, Steven Callens, Marie-Angelique De Scheerder
Summary: This study assessed the efficacy and safety of 300 mg camostat mesylate three times daily in a fasted state to treat early phase COVID-19. The results showed that camostat mesylate was not found to be effective as an antiviral drug against SARS-CoV-2.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Kuang-Yu Chen, Jayaprakash Karuppusamy, Mary B. O'Neill, Vaitea Opuu, Mathieu Bahin, Sophie Foulon, Pablo Ibanez, Lluis Quintana-Murci, Tatsuhiko Ozawa, Sylvie van der Werf, Philippe Nghe, Nadia Naffakh, Andrew Griffiths, Catherine Isel
Summary: Using a high-throughput single-cell droplet microfluidic system, researchers have quantitatively studied influenza viruses and found that genetic reassortment is non-random. They accurately predicted the fraction of pairwise segments and full genotypes and discovered that a large proportion of reassortant genotypes can emerge upon coinfection and be detected at different frequencies, highlighting the importance of systematic and exhaustive monitoring of the reassortment potential of influenza viruses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Respiratory System
Timothy Devos, Quentin Van Thillo, Veerle Compernolle, Tome Najdovski, Marta Romano, Nicolas Dauby, Laurent Jadot, Mathias Leys, Evelyne Maillart, Sarah Loof, Lucie Seyler, Martial Moonen, Michel Moutschen, Niels Van Regenmortel, Kevin K. Arien, Cyril Barbezange, Albrecht Betrains, Mutien Garigliany, Matthias M. Engelen, Iwein Gyselinck, Piet Maes, Alexander Schauwvlieghe, Laurens Liesenborghs, Ann Belmans, Peter Verhamme, Geert Meyfroidt
Summary: Transfusion of 4 units of convalescent plasma with high neutralising antibody titres early in hospitalised COVID-19 patients did not result in a significant improvement of clinical status or reduced mortality.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Public, Environmental & Occupational Health
Maria E. Goossens, Kristof Y. Neven, Pieter Pannus, Cyril Barbezange, Isabelle Thomas, Steven Van Gucht, Katelijne Dierick, Marie-Noelle Schmickler, Mathieu Verbrugghe, Nele Van Loon, Kevin K. Arien, Arnaud Marchant, Stanislas Goriely, Isabelle Desombere
Summary: The study aims to compare COVID-19 disease development and symptomatology between geriatric and younger population, showing that older individuals tend to have more symptoms before and after infection, and there are differences in the rate of asymptomatic cases between residents and staff members post-infection.
ARCHIVES OF PUBLIC HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Andrea Di Gioacchino, Rachel Legendre, Yannis Rahou, Valerie Najburg, Pierre Charneau, Benjamin D. Greenbaum, Frederic Tangy, Sylvie van der Werf, Simona Cocco, Anastassia V. Komarova
Summary: Researchers have developed a user-friendly bioinformatic tool sgDI-tector to detect and quantify sgRNA production in SARS-CoV-2 NGS data, providing an effective method for studying subgenomic RNAs of coronaviruses.