Article
Agriculture, Dairy & Animal Science
Hongjian Yan, Wanli Sun, Jinjin Wang, Qiming Gao, Yawen Zhang, Yixin Wang, Shuang Chang, Peng Zhao
Summary: Epidemiologic investigations have found an increasing detection rate of avian hepatitis E virus (HEV) in chicken flocks in China. This study used recombinant HEV open reading frames (ORF2 and ORF3) proteins to prepare specific pathogen-free (SPF) chicken serum against HEV. An SPF chicken infection model was established and samples were collected at different ages to assess HEV load using a real-time PCR assay. The study observed the therapeutic effects of antibody and interferon application on blocking vertical HEV transmission. The results showed significant reduction in HEV positivity rate when type I interferon was used alone or in combination with antisera against ORF2 and ORF3.
Article
Veterinary Sciences
Hongjian Yan, Zengna Chi, Hui Zhao, Yawen Zhang, Yuduo Zhang, Yixin Wang, Shuang Chang, Peng Zhao
Summary: This study successfully prepared avian hepatitis E virus vaccines for laying hens and broilers, and confirmed their effectiveness through challenge protection tests. The research provides technical reserve for the prevention and control of avian hepatitis E, and opens up new possibilities for vaccine development.
VETERINARY SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Michael H. Wissing, Yannick Bruggemann, Eike Steinmann, Daniel Todt
Summary: HEV is a long-neglected RNA virus and the major causative agent of acute viral hepatitis worldwide. Understanding the interactions between HEV and host cells can provide important insights for studying the viral life cycle and help in designing new therapeutic strategies.
TRENDS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Manjita Srivastava, Prudhvi Lal Bhukya, Muneesh Kumar Barman, Neha Bhise, Kavita S. Lole
Summary: Hepatitis E virus ORF3 protein interacts with host proteins to induce autophagy and promote virus replication and release. The study found that ORF3 interacts with several proteins, including DAPK1, ATG2B, ATG16L2, and histone deacetylases (HDACs), to regulate transcriptional activity, immune response, cellular processes, and autophagy. These findings highlight a novel crosstalk between ORF3-mediated autophagy and cell survival pathways.
JOURNAL OF GENERAL VIROLOGY
(2023)
Article
Virology
Gulce Sari, Jingting Zhu, Charuta Ambardekar, Xin Yin, Andre Boonstra, Zongdi Feng, Thomas Vanwolleghem
Summary: ORF3 plays a crucial role in HEV release and persistent infection, as its absence significantly decreases HEV replication and virion release. Our findings provide a rationale for targeting ORF3 as a treatment strategy for HEV infection.
JOURNAL OF VIROLOGY
(2021)
Article
Veterinary Sciences
Hanwei Jiao, Xuehong Shuai, Yichen Luo, Zhixiong Zhou, Yu Zhao, Bowen Li, Guojing Gu, Wenjie Li, Mengjuan Li, Hui Zeng, Xiaoyi Guo, Yu Xiao, Zhenhui Song, Ling Gan, Qingzhou Huang
Summary: Through high-throughput sequencing, this study identified lncRNAs and mRNAs affected by ORF3, which are involved in various disease pathways including systemic lupus erythematosus, Staphylococcus aureus infection, and regulation of stem cells. The ORF3 of the virus affects the lncRNA profile in host cells, potentially mediating the formation of obstructive jaundice by influencing bile secretion.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Keli Chai, Zhaohuan Wang, Yali Xu, Junshi Zhang, Juan Tan, Wentao Qiao
Summary: The study found that the envelope glycoprotein of bovine foamy virus is palmitoylated at amino acid positions C58 and C59, and mutations at these sites significantly inhibit viral replication.
Article
Biotechnology & Applied Microbiology
Beibei Zhang, Jie Fan, Yuhang Luo, Huanhuan Lv, Qin Zhao, Mengnan Fan, Hong Duan, Baoyuan Liu, Yuchen Nan, Yani Sun, En-Min Zhou
Summary: The study developed a competitive ELISA for detecting anti-genotype 1 HEV antibodies in human sera, which could be useful for identifying the source of infecting virus in epidemiological investigations.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cell & Tissue Engineering
Hanwei Jiao, Yu Zhao, Zhixiong Zhou, Wenjie Li, Bowen Li, Guojing Gu, Yichen Luo, Xuehong Shuai, Cailiang Fan, Li Wu, Jixuan Chen, Qingzhou Huang, Fengyang Wang, Juan Liu
Summary: The study focused on the impact of SHEV ORF3 protein on the host cell circRNA profile, further investigating its effects on lipid metabolism in HepG2 cells.
CELL TRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Haitao Liu, Xupeng Hong, Ji Xi, Stephan Menne, Jianming Hu, Joseph Che-Yen Wang
Summary: This study presents the first subnanometer-resolution structures of spherical subviral particles (SVPs) from hepatitis B virus (HBV) and woodchuck hepatitis virus (WHV) using cryo-electron microscopy and AlphaFold2 prediction. The structures reveal unique rhombicuboctahedral symmetry with 24 protruding spikes, consisting of dimer of small HBsAg with four helical domains. The lipid component in the SVPs is organized in a noncanonical lipid patch, which plays a role in particle stability. These findings provide insights into viral membrane organization and the structures of HBV and WHV spherical SVPs.
Article
Biophysics
Akhilesh Babu Ganganboina, Kenshin Takemura, Wenjing Zhang, Tian-Cheng Li, Enoch Y. Park
Summary: The study successfully developed a viral capsid-nanoparticle hybrid structure by incorporating quantum dots into virus-like particles for HEV antibody detection. The newly developed method provides an efficient route for highly sensitive and specific antibody detection in clinical diagnosis research, utilizing QDs-encapsulated VLPs as useful fluorescence emitters for biosensing platform construction.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Jerome Cattin-Ortola, Lawrence G. Welch, Sarah L. Maslen, Guido Papa, Leo C. James, Sean Munro
Summary: Researchers found that the C-terminal cytoplasmic tail of the Spike protein of SARS-CoV-2 binds trafficking machinery to ensure Spike accumulation at the site of viral budding in the Golgi, but some also traffic to the cell surface to induce syncytia formation.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Chuqi Liu, Dexiang Liu, Shen Wang, Lu Gan, Xiangliang Yang, Cong Ma
Summary: Exosomes play crucial roles in cellular communication and tumorigenesis. This study identifies SNARE proteins as the key regulators of exosome secretion in breast cancer cells and demonstrates their conserved function in different cancer types. Furthermore, targeting VAMP-7 may hold promise as a therapeutic strategy for breast cancer.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Review
Virology
Noemie Oechslin, Maliki Ankavay, Darius Moradpour, Jerome Gouttenoire
Summary: Hepatitis E virus (HEV) is now considered as one of the most frequent causes of acute hepatitis worldwide, but it has received relatively little attention for decades. Recent advances in the molecular virology of HEV have allowed study of the entire viral life cycle and exploration of host factors required for infection.
Article
Gastroenterology & Hepatology
Cedric Hartard, Honorine Fenaux, Alexis Gentilhomme, John M. Murray, Elma Akand, Elodie Laugel, Sibel Berger, Armand Maul, Alexis de Rougemont, Helene Jeulin, Thomas Remen, Mouni Bensenane, Jean-Pierre Bronowicki, Christophe Gantzer, Isabelle Bertrand, Evelyne Schvoerer
Summary: Research has identified that different variants of Hepatitis E virus (HEV) have distinct molecular patterns in humans and animals, which may impact their surface properties and transmission modes.
JOURNAL OF VIRAL HEPATITIS
(2021)
Article
Virology
Lars Maurer, Jihad El Andari, Kleopatra Rapti, Laura Spreyer, Eike Steinmann, Dirk Grimm, Viet Loan Dao Thi
Summary: This study explored a novel synthetic and myotropic Adeno-associated virus vector to induce the production of anti-ORF3 antibodies by expressing the small HEV phosphoprotein ORF3 in the musculature of mice, showing a modest inhibitory effect on the infection of quasi-enveloped HEV. The novel AAVMYO3 capsid used in this study can serve as a versatile platform for the continued development of vector-based vaccines against HEV and other infectious agents.
Article
Multidisciplinary Sciences
Gonzalo P. Solis, Arghavan Kazemzadeh, Laurence Abrami, Jana Valnohova, Cecilia Alvarez, F. Gisou van der Goot, Vladimir L. Katanaev
Summary: This study describes the inherent partitioning of G alpha oα subunit between the plasma membrane and Golgi and establishes the SwissKASH assay to probe the substrate specificity of PATs. The findings uncover the remarkable substrate selectivity of PATs in different membrane compartments, which is the basis for PMP compartmentalization.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Daniela Lenggenhager, Jonas Grossmann, Jerome Gouttenoire, Christine Sempoux, Achim Weber
Summary: Immunohistochemistry for HEV ORF2 protein is a useful tool for tissue-based diagnosis of hepatitis E. However, we report an unexpected immunoreactivity of the HEV ORF2 antibody to CMV proteins. The staining pattern of CMV-infected cells is limited to the nucleus, in contrast to the cytoplasmic and occasional canalicular positivity observed in hepatitis E.
Article
Multidisciplinary Sciences
Laia Simo-Riudalbas, Sandra Offner, Evarist Planet, Julien Duc, Laurence Abrami, Sagane Dind, Alexandre Coudray, Mairene Coto-Llerena, Caner Ercan, Salvatore Piscuoglio, Claus Lindbjerg Andersen, Jesper Bertram Bramsen, Didier Trono
Summary: The early diagnosis of colorectal cancer (CRC) is an unmet medical need. In this study, the authors characterized cancer-specific transposable element-driven transpochimeric gene transcripts (TcGTs) and identified the role of POU5F1B in CRC growth and metastasis. They found that the expression of POU5F1B, driven by a primate-specific endogenous retroviral promoter, is a strong negative prognostic biomarker in CRC. They also demonstrated that POU5F1B promotes CRC cell proliferation and metastatic potential by inducing intracellular signaling events and the release of trans-acting factors involved in cell growth and adhesion. These findings provide interesting leads for the development of cancer therapies.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Patrick A. Sandoz, Robin A. Denhardt-Eriksson, Laurence Abrami, Luciano A. Abriata, Gard Spreemann, Catherine Maclachlan, Sylvia Ho, Beatrice Kunz, Kathryn Hess, Graham Knott, Francisco S. Mesquita, Vassily Hatzimanikatis, F. Gisou van der Goot
Summary: The study reveals that S-acylation of CLIMP-63 regulates its function in the formation of endoplasmic reticulum sheets by mediating its oligomerisation, turnover, and localisation.
NATURE COMMUNICATIONS
(2023)
Editorial Material
Gastroenterology & Hepatology
Jerome Gouttenoire, Johan Neyts
Review
Virology
Noemie Oechslin, Maliki Ankavay, Darius Moradpour, Jerome Gouttenoire
Summary: Hepatitis E virus (HEV) is now considered as one of the most frequent causes of acute hepatitis worldwide, but it has received relatively little attention for decades. Recent advances in the molecular virology of HEV have allowed study of the entire viral life cycle and exploration of host factors required for infection.
Article
Biochemistry & Molecular Biology
Francisco S. Mesquita, Laurence Abrami, Arthur Samurkas, F. Gisou van Der Goot
Summary: S-acylation is a covalent modification of proteins with fatty acids, allowing dynamic control of protein properties and functions in association with cell membranes. It regulates a substantial portion of the human proteome and has increasingly recognized roles in protein lifespan. Recent advancements have provided new insights into its mechanisms and implications. This review focuses on the importance of S-acylation in the homeostasis, function, and coordination of integral membrane proteins.
Review
Cell Biology
Muhammad U. Anwar, F. Gisou van der Goot
Summary: S-acylation is an important posttranslational modification that regulates cellular processes. This reversible lipid modification affects cellular pathways and physiological processes, and the enzymes and proteins involved in S-acylation are still being discovered and studied.
JOURNAL OF CELL BIOLOGY
(2023)
Meeting Abstract
Medicine, General & Internal
Maliki Ankavay, Nathalie Da Silva, Noemie Oechslin, Katja Dinkelborg, Patrick Behrendt, Darius Moradpour, Jerome Gouttenoire
SWISS MEDICAL WEEKLY
(2022)
Meeting Abstract
Medicine, General & Internal
Noemie Oechslin, Nathalie Da Silva, Maliki Ankavay, Angela Pollan, Darius Moradpour, Jerome Gouttenoire
SWISS MEDICAL WEEKLY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Noemie Oechslin, Nathalie Da Silva, Maliki Ankavay, Darius Moradpour, Jerome Gouttenoire
JOURNAL OF HEPATOLOGY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Jerome Gouttenoire, Roland Sahli, Daniela Muellhaupt, Montserrat Fraga Christinet, Darius Moradpour
JOURNAL OF HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Cindy Zhang, Andrew Freistaedter, Carolin Schmelas, Manuel Gunkel, Viet Loan Dao Thi, Dirk Grimm
Summary: Researchers engineered AAV6 vectors to express shRNAs targeting HEV transcripts, identifying three potent shRNAs that reduced replication by up to 95%. Multiplexing these shRNAs further enhanced their inhibitory potency over a prolonged period, demonstrating potential for clinical translation in suppressing HEV replication.
HEPATOLOGY COMMUNICATIONS
(2022)