Article
Cell Biology
Wenfei Pan, Limei Deng, Haitao Wang, Vivien Ya-Fan Wang
Summary: The NF-kappa B family of dimeric transcription factors regulate various biological functions. A study has revealed that the atypical I kappa B protein, Bcl3, plays a crucial role in enhancing the population of the p52:p52 homodimer within the NF-kappa B family. Bcl3 competes with other NF-kappa B subunits for efficient p52:p52 homodimer formation, leading to the upregulation of target genes involved in cell proliferation, migration, and inflammation. The aberrant activation of Bcl3 and p52 contributes to cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Dianbo Long, Yiyang Xu, Guping Mao, Ruobing Xin, Zengfa Deng, Hongyi Liao, Zhiwen Li, Zhi Yang, Baoxi Yu, Zhijian Yang, Aishan He, Ziji Zhang, Yan Kang
Summary: tRNA-derived fragments (tRFs) have been shown to play important roles in cell metabolism by regulating gene expression. This study investigates the role of tRFs in the cellular metabolism of the anterior cruciate ligament (ACL). The findings reveal that TRF365 regulates ACL cell metabolism by targeting IKBKB.
CELL DEATH DISCOVERY
(2022)
Article
Microbiology
Tao Wang, Rui Luo, Jing Zhang, Zhanhao Lu, Lian-Feng Li, Yong-Hui Zheng, Li Pan, Jing Lan, Huanjie Zhai, Shujian Huang, Yuan Sun, Hua-Ji Qiu
Summary: In this study, MGF300-2R was identified as a key factor for replication and pathogenicity of African swine fever virus (ASFV). MGF300-2R promotes the K27-linked ubiquitination of IKKα and IKKβ for TOLLIP-dependent autophagic degradation, suppressing the activation of the NF-κB signaling pathway. These findings highlight the relevance of the inflammatory response in ASFV virulence and suggest MGF300-2R as a potential target for vaccine development.
Article
Cell Biology
Panagiotis Tsapras, Stavroula Petridi, Selina Chan, Marta Geborys, Anne-Claire Jacomin, Antonia P. Sagona, Pascal Meier, Ioannis P. Nezis
Summary: Selective autophagy regulates innate immune responses in Drosophila by removing the Tak1/Tab2 signaling complex, preventing constitutive activation of the IMD pathway.
Article
Biology
Huachun Cui, Sami Banerjee, Na Xie, Tapan Dey, Rui-Ming Liu, Yan Y. Sanders, Gang Liu
Summary: This study demonstrates that MafB is a critical negative regulator of the NLRP3 inflammasome in macrophages. By sustaining the expression of p62, MafB inhibits inflammasome activation. Loss of MafB enhances NLRP3 inflammasome activation and contributes to the development of immune disorders.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jugal Kishore Das, Yijie Ren, Anil Kumar, Hao-Yun Peng, Liqing Wang, Xiaofang Xiong, Robert C. Alaniz, Paul de Figueiredo, Xingcong Ren, Xiaoqi Liu, Alexey G. Ryazonov, Jin-Ming Yang, Jianxun Song
Summary: eEF-2K plays a critical role in regulating the fate and function of CD8(+) T cells. Loss of eEF-2K leads to increased proliferation but decreased survival of CD8(+) T cells, along with premature senescence and exhaustion. eEF-2K deficiency also affects the metabolic activity of CD8(+) T cells and the effectiveness of CAR-T therapy against cancer.
Article
Fisheries
Chen Chen, Liangli Yang, Muhammad Nadeem Abbas, Deng Zou, Jun Li, Xuexia Geng, Haijun Zhang, Yuxuan Sun
Summary: This study reveals the importance of autophagy in innate immunity and its relationship with LPS-induced immune response in A. pernyi. The findings suggest that Relish gene regulates the transcription of ATG5 to eliminate Gram-negative bacteria through anti-microbial autophagy.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Cell Biology
Qiudong Yang, Wenhua Zhao, Yuyi Chen, Yue Chen, Jiali Shi, Ran Qin, Hua Wang, Ruixia Wang, Hua Yuan, Wen Sun
Summary: The study reveals that the RelA/miR-30a/NLRP3 signaling axis is involved in rheumatoid arthritis by regulating the NLRP3 inflammasome in macrophages.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Enping Liu, Jiawei Sun, Jing Yang, Lin Li, Qili Yang, Jiuqin Zeng, Jiayu Zhang, Dahua Chen, Qinmiao Sun
Summary: ZDHHC11 positively regulates NF-kappa B signaling by promoting TRAF6 oligomerization and ligase activity, subsequently activating TAK1 and IKK complexes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Yu Zhang, Zhuo-Chang Chen, Nan Li, Zhi-Hua Wang, Ya-Li Guo, Cui-Jie Tian, Dong-Jun Cheng, Xue-Yi Tang, Luo-Xian Zhang
Summary: Activated neutrophil-derived CRNDE is transferred to ASMCs through exosomes, activating the NF-kappa B pathway and promoting the proliferation and migration of ASMCs, which contributes to airway remodeling in asthma.
HUMAN MOLECULAR GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Yongguang Zhang, Dongya Cui, Miaohui Huang, Yongwei Zheng, Baijiao Zheng, Liling Chen, Qi Chen
Summary: The protein NONO is a multifunctional nuclear protein that regulates transcriptional regulation, mRNA splicing, and DNA repair. This study shows that NONO has a critical role in B-cell development and B-cell activation. Deletion of NONO impairs early B-cell development and B-cell maturation, as well as the activation of pathways involved in B-cell activation, such as ERK, AKT, and NF-kappa B.
Article
Pharmacology & Pharmacy
Wenbin Pei, Xiaojian Huang, Bowei Ni, Rui Zhang, Guangyi Niu, Hongbo You
Summary: In this study, it was found that Alantolactone (ALT) could alleviate IL-1 beta-induced inflammatory responses, protect cartilage from damage, and promote impaired autophagy through inhibiting the STAT3 and NF-kappa B signal pathways, suggesting a promising therapeutic effect for osteoarthritis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Minjun Yao, Caihua Zhang, Lingzhi Ni, Xiaoxiao Ji, Jianqiao Hong, Yazhou Chen, Jie Wang, Congsun Li, Jiyan Lin, Tingting Lu, Yihao Sheng, Menghao Sun, Mingmin Shi, Chenhe Zhou, Xunzi Cai
Summary: This study demonstrated for the first time the protective medicinal efficacy of cepharanthine (CEP) in osteoarthritis. CEP suppressed inflammation and increased protein expression in joint tissues while restoring cellular autophagy. The mouse model confirmed the therapeutic potential of CEP in osteoarthritis treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Gastroenterology & Hepatology
Tobias Riedl, Suzanne Faure-Dupuy, Maude Rolland, Svenja Schuehle, Zohier Hizir, Silvia Calderazzo, Xiaodong Zhuang, Jochen Wettengel, Martin Alexander Lopez, Romain Barnault, Valbona Mirakaj, Sandra Prokosch, Danijela Heide, Corinna Leuchtenberger, Martin Schneider, Bernd Hessling, Benjamin Stottmeier, Isabel M. Wessbecher, Peter Schirmacher, Jane A. McKeating, Ulrike Protzer, David Durantel, Julie Lucifora, Emmanuel Dejardin, Mathias Heikenwalder
Summary: The stabilization of HIF1 alpha was found to inhibit the expression of APOBEC3B and its antiviral effects against HBV. Inhibiting the expression or stabilization of HIF1 alpha could serve as an anti-HBV strategy. High levels of HIF1 alpha may provide a reservoir for HBV survival in immune-active patients, and therefore should be considered as a constraining factor in the development of immune therapies.
Article
Gastroenterology & Hepatology
Yasir J. Abozaid, Ibrahim Ayada, Laurens A. van Kleef, Costanza L. Vallerga, Qiuwei Pan, Willem P. Brouwer, M. Arfan Ikram, Joyce Van Meurs, Robert J. de Knegt, Mohsen Ghanbari
Summary: This study aimed to identify plasma proteomics associated with fatty liver disease (FLD) and fibrosis using population-based data. The results showed that 27 proteins were significantly associated with FLD, including FGF-21 and CES1. In addition, the expression levels of IL-18R1 and CES1 were upregulated in an FLD model of human liver organoids. These findings suggest the importance of plasma proteins in the pathogenesis of FLD and fibrosis.
Article
Biology
Wenjing Zheng, Florentina Rus, Ana Hernandez, Ping Kang, William Goldman, Neal Silverman, Marc Tatar
Article
Immunology
Ashley E. Nazario-Toole, Javier Robalino, Kwame Okrah, Hector Corrada-Bravo, Stephen M. Mount, Louisa P. Wu
JOURNAL OF IMMUNOLOGY
(2018)
Article
Microbiology
Cara West, Neal Silverman
Article
Multidisciplinary Sciences
Athanassios D. Velentzas, Panagiotis D. Velentzas, Stamatia A. Katarachia, Athanasios K. Anagnostopoulos, Niki E. Sagioglou, Eleni V. Thanou, Maria M. Tsioka, Vassiliki E. Mpakou, Zoe Kollia, Vassilios E. Gavriil, Issidora S. Papassideri, George Th. Tsangaris, Alkiviadis-Constantinos Cefalas, Evangelia Sarantopoulou, Dimitrios J. Stravopodis
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Panagiotis D. Velentzas, Lejie Zhang, Gautam Das, Tsun-Kai Chang, Charles Nelson, William R. Kobertz, Eric H. Baehrecke
DEVELOPMENTAL CELL
(2018)
Article
Virology
Cara West, Florentina Rus, Ying Chen, Anni Kleino, Monique Gangloff, Don B. Gammon, Neal Silverman
Article
Cell Biology
Elizabeth A. Allen, Clelia Amato, Tina M. Fortier, Panagiotis Velentzas, Will Wood, Eric H. Baehrecke
JOURNAL OF CELL BIOLOGY
(2020)
Article
Developmental Biology
Geanette Lam, Hyuck-Jin Nam, Panagiotis D. Velentzas, Eric H. Baehrecke, Carl S. Thummel
Summary: Research shows that the E93 gene plays a crucial role in Drosophila development and morphogenesis, especially in the regulation of salivary glands. E93 is specifically induced in late prepupae, providing a critical signal that defines the end of larval development and the onset of adult differentiation.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kendi Okuda, Miriam Maria Silva Costa Franco, Ari Yasunaga, Ricardo Gazzinelli, Michel Rabinovitch, Sara Cherry, Neal Silverman
Summary: Leishmania parasites utilize virulence mechanisms to invade and replicate in macrophages, inhibiting host defenses and altering gene expression to enhance their survival and replication.
Article
Genetics & Heredity
Lu Wang, Lauren Tracy, Weijia Su, Fu Yang, Yu Feng, Neal Silverman, Z. Z. Zhao Zhang
Summary: Activation of mdg4 retrotransposons during Drosophila metamorphosis can educate the host's innate immune system and provide long-term protection against viral infections.
Article
Biochemistry & Molecular Biology
Ravi Bharadwaj, Madison V. Anonick, Swati Jaiswal, Siavash Mashayekh, Ashley Brown, Kimberly A. Wodzanowski, Kendi Okuda, Neal Silverman, Catherine L. Grimes
Summary: NOD1 and NOD2 innate immune sensing is a critical host response, but the subcellular trafficking and transport mechanisms by which muropeptides access these cytosolic innate immune receptors are not well understood. This study synthesized and validated alkyne-modified muropeptides for use in click-chemistry reactions, providing valuable tools to decipher the underlying cell biology and biochemistry of NOD1 and NOD2 innate immune sensing.
Article
Biochemical Research Methods
Panagiotis D. Velentzas, Eric H. Baehrecke
Summary: This protocol outlines the embedding and processing of Drosophila pupae in paraffin to monitor tissue changes during development. By focusing on developmental clearance of larval salivary glands, detailed changes during metamorphosis can be observed.