Article
Oncology
Ilaria Nesi, Chiara Della Bella, Maria Letizia Taddei, Alice Santi, Erica Pranzini, Paolo Paoli, Mario Milco D'Elios, Matteo Ramazzotti, Massimo Genovese, Anna Caselli, Paolo Cirri
Summary: Early-stage tumors become invisible to the immune system, and clinical treatments become ineffective when the immune system fails. Restoring the natural anti-tumor role of the immune system could be a promising approach for cancer treatment.
FRONTIERS IN ONCOLOGY
(2023)
Review
Physiology
Derek W. Russell, Kristopher R. Genschmer, J. Edwin Blalock
Summary: Chronic obstructive pulmonary disease (COPD) is a complex smoking-related disease driven by interrelated processes. Extracellular vesicles (EVs) are powerful effectors that could play a significant role in COPD pathogenesis.
ANNUAL REVIEW OF PHYSIOLOGY
(2022)
Article
Oncology
Ilaria Giusti, Marianna Di Francesco, Giuseppina Poppa, Letizia Esposito, Sandra D'Ascenzo, Vincenza Dolo
Summary: Fibroblasts in the tumor microenvironment can be activated by cancer cells to become cancer-associated fibroblasts (CAFs), which promote tumor progression by remodeling the tumor stroma. Extracellular vesicles (EVs), including exosomes (EXOs) and microvesicles (MVs), play a crucial role in the communication between cancer cells and fibroblasts. Different subpopulations of EVs have different effects on fibroblasts, and the secretome of activated fibroblasts can modulate the response of bystander cells, indicating the important role of EVs in the mutual cross-talk between tumor cells and CAFs.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell & Tissue Engineering
Riccardo Bazzoni, Ilaria Tanasi, Nice Turazzi, Mauro Krampera
Summary: Extracellular vesicles (EVs) are released by cells and contain various bioactive molecules, playing a crucial role in intercellular communication and biological processes. In hematological malignancies, EVs contribute to tumor development, drug resistance, and bone marrow microenvironment modulation. EVs have potential as diagnostic biomarkers, therapeutic targets, and drug carriers.
Article
Biochemistry & Molecular Biology
Tatyana Chernova, Stefano Grosso, Xiao-Ming Sun, Angela Rubio Tenor, Joaquin Zacarias Cabeza, Andrew Craxton, Emily L. Self, Apostolos Nakas, Kelvin Cain, Marion MacFarlane, Anne E. Willis
Summary: Research has found that extracellular vesicles (CAF-EVs) derived from carcinoma-associated fibroblasts (CAFs) promote the growth and invasion of malignant mesothelioma. Treatment with Simvastatin, a statin drug, can suppress the pro-oncogenic effects of CAF-EVs, providing new targets for cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Cell Biology
Kenneth W. Witwer, Deborah C. Goberdhan, Lorraine O'Driscoll, Clotilde Thery, Joshua A. Welsh, Cherie Blenkiron, Edit Buzas, Dolores Di Vizio, Uta Erdbrugger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Jan Lotvall, Rienk Nieuwland, Takahiro Ochiya, Sophie Rome, Susmita Sahoo, Lei Zheng
Summary: The MISEV initiative by ISEV aims to advance EV science globally, with the 2018 release of MISEV2018 providing comprehensive recommendations on EV nomenclature, sample processing, separation concentration, characterization, functional studies, and reporting requirements, with input from over 400 scientists. ISEV sought feedback and opinions from the community through a survey to better formulate MISEV and address key issues.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Oncology
Kellen Wright, Thuc Ly, Matthew Kriet, Andras Czirok, Sufi Mary Thomas
Summary: Cancer cells rely on the tumor microenvironment (TME) for growth and metastasis. Among the components of TME, cancer-associated fibroblasts (CAFs) play a crucial role. CAFs are heterogeneous and can remodel the microenvironment, as well as communicate with immune cells to aid in immune evasion. This review focuses on the factors and signaling pathways of CAFs.
Review
Oncology
Larissa Kotelevets, Eric Chastre
Summary: Extracellular vesicles, such as exosomes, ectosomes, and apoptotic bodies, play a critical role in intercellular communication and can be used for noninvasive diagnosis of cancer. They can also be engineered for efficient delivery of therapeutic agents for tumor treatment. This review highlights the importance of extracellular vesicles in colorectal cancer progression, diagnosis, and potential therapeutic approaches.
Review
Medicine, Research & Experimental
Artur Slomka, Bingduo Wang, Tudor Mocan, Adelina Horhat, Arnulf G. Willms, Ingo G. H. Schmidt-Wolf, Christian P. Strassburg, Maria A. Gonzalez-Carmona, Veronika Lukacs-Kornek, Miroslaw T. Kornek
Summary: Liquid biopsies, as biomarkers in cancer diagnosis, have great potential but also limitations. This review focuses on comparing extracellular vesicles (EVs) and circulating tumor cells (CTCs) and discussing their similarities in characterization and quantification. While it is not concluded which is better between CTCs and EVs, the review provides reasons to intensify research on both.
Review
Cell Biology
Xinyi Hu, Yukang Lu, Jiajun Zhou, Lanfeng Wang, Mengting Zhang, Yiping Mao, Zhiping Chen
Summary: Colorectal cancer is a prevalent malignant tumor with high mortality and incidence rates. Small extracellular vesicles (sEVs) play a significant role in the communication and progression of cancer cells. Regulatory RNAs have emerged as potential biomarkers for the diagnosis and prognosis of colorectal cancer, influencing various cancer-related processes. This review highlights the importance of sEVs as biomarkers in colorectal cancer diagnosis and suggests their potential application in treatment, emphasizing the need for further research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Biology
Xuewei Qi, Shuzhen Chen, Huisi He, Wen Wen, Hongyang Wang
Summary: This review summarizes the role of extracellular vesicles (EVs) in the tumor microenvironment during the development and progression of liver cancer, highlighting their potential as liquid biopsy biomarkers for diagnosis and prognosis. The study also emphasizes the progress of EVs as antigen carriers and EV-based therapeutics in preclinical studies of liver cancer.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Review
Physiology
Giulia Biffi, David A. Tuveson
Summary: Efforts to develop anti-cancer therapies have mainly targeted the epithelial compartment, but recent studies have shown the significant influence of cancer-associated fibroblasts (CAFs) in tumor progression. CAFs not only promote cancer cell proliferation, therapy resistance, and immune exclusion, but may also restrain tumor progression in certain contexts. Research on CAFs has focused on their heterogeneity, plasticity, and functions across different cancer types and states, as well as advancements in therapeutic strategies targeting CAFs currently undergoing preclinical and clinical evaluation.
PHYSIOLOGICAL REVIEWS
(2021)
Article
Oncology
Kyohei Yugawa, Tomoharu Yoshizumi, Yohei Mano, Shinji Itoh, Noboru Harada, Toru Ikegami, Kenichi Kohashi, Yoshinao Oda, Masaki Mori
Summary: The study revealed that the loss of miR-150-3p in CAFs-derived exosomes significantly promotes HCC progression. Exosomal miR-150-3p may serve as a potential prognostic biomarker, and transferring miR-150-3p-loaded exosomes to HCC cells could be a novel therapeutic option.
Article
Biochemistry & Molecular Biology
Chunmei Zhang, Meiying Qin
Summary: This review examines the significant role of extracellular vesicles (EVs) in ovarian cancer, highlighting their potential as diagnostic markers and therapeutic targets. The comprehensive analysis explores the mechanisms by which EVs interact with the tumor microenvironment, including the regulation of tumor growth through EVs carrying matrix metalloproteinases (MMPs) and EV-mediated inhibition of angiogenesis. The potential clinical applications of EVs targeting the tumor microenvironment in ovarian cancer are discussed, including the development of novel treatment strategies and identification of new biomarkers for early detection and prognosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Medicinal
Marcello Casertano, Massimo Genovese, Paolo Paoli, Alice Santi, Anna Aiello, Marialuisa Menna, Concetta Imperatore
Summary: The chemical investigation of Clavelina lepadiformis, a Mediterranean ascidian, led to the discovery of a new compound named lepadin L, along with two known metabolites, lepadins A and B. The structure and configuration of lepadin L were determined using HR-ESIMS and NMR spectroscopy. Further analysis showed that lepadin A had strong cytotoxic activity and could inhibit cell migration and clonogenity in A375 cells.
Article
Biochemistry & Molecular Biology
Elena Michelucci, Silvia Rocchiccioli, Melania Gaggini, Rudina Ndreu, Sergio Berti, Cristina Vassalle
Summary: This study found that specific ceramide species are associated with cardiovascular risk, inflammation, and disease severity in acute myocardial infarction (AMI). A detailed analysis of ceramides may help to better understand cardiovascular pathobiology and suggest these new biomarkers as possible risk predictors and pharmacological targets in AMI patients.
Article
Biochemistry & Molecular Biology
Costanza Vanni, Francesca Clemente, Paolo Paoli, Amelia Morrone, Camilla Matassini, Andrea Goti, Francesca Cardona
Summary: The synthesis of five new multivalent derivatives of a trihydroxypiperidine iminosugar was achieved through copper catalyzed alkyne-azide cycloaddition. These multivalent compounds showed much more potent inhibitory effects on lysosomal GCase, with the trivalent derivative demonstrating a 2-fold recovery of enzyme activity on Gaucher patient fibroblasts. Furthermore, thermal denaturation experiments indicated its ability to enhance stability of the recombinant enzyme used in therapy.
Review
Biochemistry & Molecular Biology
Melania Gaggini, Rudina Ndreu, Elena Michelucci, Silvia Rocchiccioli, Cristina Vassalle
Summary: This review presents evidence on the structure and generation of ceramides, and discusses their role in cardiometabolic disease, as well as the current translational opportunities and challenges for ceramide application in the cardiometabolic clinical settings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nicoletta Di Giorgi, Antonella Cecchettini, Elena Michelucci, Giovanni Signore, Elisa Ceccherini, Francesco Ferro, Elena Elefante, Chiara Tani, Chiara Baldini, Silvia Rocchiccioli
Summary: In this study, mass spectrometry was used to analyze the salivary proteomics of patients with established primary Sjogren's syndrome (pSS) and patients with pre-clinical SS. A common protein signature was identified in their salivary fluid. Differential expression of inflammatory, immunity-related, and acinar proteins suggests that saliva may reflect exocrine gland inflammation in the early phases of the disease. Salivary proteomics can be a valuable tool for identifying reliable biomarkers for SS, even in the preclinical phase of the disease.
Article
Chemistry, Medicinal
Marcello Casertano, Massimo Genovese, Lucia Piazza, Francesco Balestri, Antonella Del Corso, Alessio Vito, Paolo Paoli, Alice Santi, Concetta Imperatore, Marialuisa Menna
Summary: Diabetes mellitus is a complex metabolic disorder that poses a global health problem. This study identified protein tyrosine phosphatase 1B and aldose reductase as important targets in the development of type 2 diabetes and its chronic complications. Through fragment-based screening and rational design of analogues, the most potent inhibitors were discovered.
Article
Biology
Elisa Ceccherini, Nicoletta Di Giorgi, Elena Michelucci, Giovanni Signore, Lorena Tedeschi, Federico Vozzi, Silvia Rocchiccioli, Antonella Cecchettini
Summary: The study revealed that human cholangiocytes exhibit reduced proliferation and cell cycle arrest in G0/G1 phase following TGF-beta treatment. Proteomic analysis identified four downregulated proteins related to Ca2+ homeostasis.
Meeting Abstract
Cardiac & Cardiovascular Systems
S. Rocchiccioli, N. Di Giorgi, E. Michelucci, G. Signore, A. J. H. A. Scholte, J. Knuuti, R. R. Buechel, A. Teresinska, M. N. Pizzi, A. Roque, R. Poddighe, O. Parodi, G. Pelosi, D. Neglia, C. Caselli
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Gabriele Nieddu, Elena Michelucci, Marilena Formato, Cristina Ciampelli, Gabriele Obino, Giovanni Signore, Nicoletta Di Giorgi, Silvia Rocchiccioli, Antonio Junior Lepedda
Summary: Carotid atherosclerosis is a significant healthcare problem, and there is a need for reliable molecular markers to identify high-risk patients. This study used plasma lipoprotein lipidomics to identify specific lipidomic patterns associated with carotid plaque instability. The results show promising new biomarkers of potential clinical relevance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Francesca Clemente, Maria Giulia Davighi, Camilla Matassini, Francesca Cardona, Andrea Goti, Amelia Morrone, Paolo Paoli, Tomas Tejero, Pedro Merino, Martina Cacciarini
Summary: Piperidine-based photoswitchable derivatives have been developed as potential pharmacological chaperones for Gaucher disease. The compounds inhibit human GCase in a non-competitive manner, with the interaction occurring at an allosteric site. Experiments on GD patients' fibroblasts showed a moderate activity enhancement of the mutant GCase, indicating the potential for further improvement.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Melania Gaggini, Elena Michelucci, Rudina Ndreu, Silvia Rocchiccioli, Kyriazoula Chatzianagnostou, Sergio Berti, Cristina Vassalle
Summary: This study assessed the correlation between ceramides and cardiometabolic diseases such as acute myocardial infarction and type 2 diabetes. The results showed that ceramides were not strongly associated with myocardial infarction, but specific species were elevated in type 2 diabetes-related myocardial infarction. Additionally, certain ceramides were correlated with other cardiometabolic risk factors. Thus, ceramide assessment may provide insights into the molecular mechanisms underlying acute cardiac events and cardiometabolic risk, potentially serving as prognostic predictors and therapeutic targets in type 2 diabetes-related myocardial infarction patients.
Article
Agriculture, Dairy & Animal Science
F. Scicutella, M. A. Cucu, F. Mannelli, R. Pastorelli, M. Daghio, P. Paoli, L. Pazzagli, L. Turini, A. Mantino, S. Luti, M. Genovese, C. Viti, A. Buccioni
Summary: The study tested the use of olive oil pomace (OOP) produced by a new technology in dairy cow feeding and its effects on animal performance, rumen microbiota, biohydrogenation processes, and milk quality. The results showed that adding OOP improved the nutritional quality of milk and increased important functional fatty acid content without affecting animal performance and milk production.
Article
Biochemistry & Molecular Biology
Elisa Ceccherini, Giovanni Signore, Lorena Tedeschi, Federico Vozzi, Nicoletta Di Giorgi, Elena Michelucci, Antonella Cecchettini, Silvia Rocchiccioli
Summary: In this study, a water-stable and freely dispersible nanoformulation of curcumin was prepared and characterized. The effects of this nanoformulation on cholangiocytes under fibrotic pathological conditions were investigated. The results showed that curcumin nanoformulation exhibited anti-inflammatory properties and induced programmed cell death to counteract cellular damages induced by TGF-beta. It also positively influenced the expression of proteins involved in TGF-beta-mediated fibrosis. These findings provide insights into the mechanisms associated with cholangiopathies and potential for targeted therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Costanza Cicchi, Paolo Paoli, Alessandra Modesti, Federica Mannelli, Federica Scicutella, Arianna Buccioni, Carolina Fontanarosa, Simone Luti, Luigia Pazzagli
Summary: Transitioning to sustainable food systems involves the reuse of agricultural by-products as alternative feed ingredients, such as olive oil pomace, which can be used in the diet of lactating cows. Olive oil pomace supplementation does not negatively impact animal performance or rumen microbiota and even improves the fatty acid content of milk. The peptides derived from milk proteins of cows fed with olive oil pomace have potential antioxidant, anti-inflammatory, and anti-cancer effects, making them bioactive peptides. Incorporating agro-industrial by-products into dairy cow feeding strategies is a viable option for sustainable agriculture.
Article
Chemistry, Organic
Francesca Buco, Camilla Matassini, Costanza Vanni, Francesca Clemente, Paolo Paoli, Cosimo Carozzini, Alice Beni, Francesca Cardona, Andrea Goti, Sergio Enrique Moya, Maria Grazia Ortore, Patrizia Andreozzi, Amelia Morrone, Marco Marradi
Summary: In this study, gold nanoparticles decorated with monosaccharides or sulfated ligands were designed as multivalent inhibitors for the enzyme GALNS associated with Morquio A. The beta-D-galactoside-coated AuNPs showed the most effective inhibition of GALNS activity. In the case of mixed-ligand AuNPs, alpha-D-mannosides exhibited a synergistic effect with sulfated ligands, leading to significantly stronger inhibition of GALNS.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)