Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Yanbing Lv, Yucheng Yuan, Ning Hu, Na Jin, Dangdang Xu, Ruili Wu, Huaibin Shen, Ou Chen, Lin Song Li
Summary: This study presents a synthesis method for ultra-thick shell quantum dots and their application in C-reactive protein detection, achieving nearly a 3-fold enhancement in detection sensitivity and a 2-fold increase in shelf life. The performance improvements in protein detection are attributed to the use of core/shell quantum dots with high particle stability, high PL QYs, and a large surface area conducive to surface antibody coupling.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Physical
Yu Knyazev, D. A. Balaev, S. Stolyar, O. A. Bayukov, R. N. Yaroslavtsev, V. P. Ladygina, D. A. Velikanov, R. S. Iskhakov
Summary: This study investigates the biogenic ferrihydrite nanoparticles and reveals the exceptional magnetic anisotropy formed by the highly developed surface of ferrihydrite nanoparticles. A core-shell structural model of the biogenic ferrihydrite particles is proposed based on Mossbauer data analysis.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Chemistry, Multidisciplinary
Xifang Chen, Yue Zhou, Hongwen Han, Xiaoyu Wang, Lei Zhou, Zao Yi, Zhenjin Fu, Xianwen Wu, Gongfa Li, Liangcai Zeng
Summary: This study investigated the effects of carbon shell coating on the optical and magnetic properties of small size Fe3O4 nanoparticles. The results showed that carbon coating can enhance the absorption intensity in the UV-visible light region and reduce surface uniaxial anisotropy, leading to a decrease in the average blocking temperature.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Chemistry, Analytical
Young Jae Choi, Jong Uk Lee, Sang Jun Sim
Summary: Autophagy is a crucial cellular process in various diseases, and the quantification of LC3 plays a key role in autophagy research. A gold nanoparticle-based LC3 sensing platform provides a simple, rapid, and highly sensitive method for measuring autophagy flux.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Electrical & Electronic
Mohammadrahim Kazemzadeh, Colin L. Hisey, Priscila Dauros-Singorenko, Simon Swift, Kamran Zargar-Shoshtari, Weiliang Xu, Neil G. R. Broderick
Summary: Bacterial extracellular vesicles (EVs) are nano-scale lipid-enclosed packages that play important roles in various biological processes. This study demonstrates the potential of using surface-enhanced Raman spectroscopy (SERS) and machine learning algorithms to rapidly classify and characterize bacterial EVs, which is crucial for the development of their laboratory and clinical applications.
IEEE SENSORS JOURNAL
(2022)
Article
Chemistry, Physical
Biljana Pejova, Simona Premcheska, Emel Sherif Miftar
Summary: A controllable ultrasound-assisted synthesis method is developed for depositing elemental silver and core-shell Ag@Ag2O nanoparticles in thin-film form on glass substrates. The synthesis method utilizes ultrasonic irradiation to modify the reaction mechanism and precisely control the composition and packing of the nanoparticles. The synthesized materials have nano-sized crystal radii, and the films exhibit different morphologies depending on the type of nanoparticles used. The properties and behaviors of the films are influenced by factors such as particle size, dispersion, plasmon coupling, and nanocrystal-substrate interaction.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Polymer Science
Yi-Jung Lu, Han-Yun Hsieh, Wen-Chang Kuo, Pei-Kuen Wei, Horn-Jiunn Sheen, Hidetoshi Tahara, Te-Wei Chiu, Yu-Jui Fan
Summary: The study proposed a multiplex detection system by integrating LSPR sensing array and microfluidic channels, proving successful in detecting different targets in parallel channels.
Article
Biochemical Research Methods
Wihan Adi, Dhruv Biswas, Miriam A. Shelef, Filiz Yesilkoy
Summary: The study presents a multiplexed and label-free nanoplasmonic biosensor that can be used for rapid antibody analysis. This method allows for simultaneous quantification of antigen-specific antibody response against SARS-CoV-2 spike and nucleocapsid proteins in serum, demonstrating high sensitivity and specificity for past infection diagnosis.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Physical
G. B. Oliveira-Filho, J. J. Atoche-Medrano, F. F. H. Aragon, J. C. Mantilla Ochoa, D. G. Pacheco-Salazar, S. W. da Silva, J. A. H. Coaquira
Summary: Core-shell structure Au@Fe3O4 nanoparticles were synthesized using a thermal decomposition method, with a core diameter of approximately 10.5 nm and a shell thickness of roughly 1.85 nm. The nanocomposite exhibited a successful coupling of Au and Fe3O4, possessing good crystal structure and magnetic response characteristics.
APPLIED SURFACE SCIENCE
(2021)
Review
Nanoscience & Nanotechnology
Ali Shafiee, Navid Rabiee, Sepideh Ahmadi, Marzieh Baneshi, Mehrdad Khatami, Siavash Iravani, Rajender S. Varma
Summary: Hybrid nanostructures and core-shell nanostructures possess unique physicochemical properties, making them suitable for various applications such as organic pollutant degradation and energy storage. Various techniques can be used to synthesize core-shell nanostructures, which offer high stability and reproducibility.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Bowen He, Xi Liu, Liwei Chen
Summary: By using advanced transmission electron microscope techniques, two alternative particle attachment growth pathways dominating the growth of Au@Ag core-shell nanocuboids are directly observed. One pathway involves the in situ reduction of AgCl nanoparticles attached to Au nanorods and the subsequent epitaxial growth of the Ag shell. The other pathway involves the adherence of Ag-AgCl Janus nanoparticles to Au nanorods with random orientations, followed by nanoparticle redispersion and the resulting formation of epitaxial Ag shells on the Au nanorods. The particle-mediated growth of Ag shells is accompanied by the redispersion of surface atoms, tending to form a uniform structure, providing a new mechanistic understanding of core-shell nanostructure synthesis.
Article
Chemistry, Analytical
Sommer Osman, Echo L. L. Claus, Robbyn K. K. Anand
Summary: In this study, a microfluidic bead-based lateral flow immunoassay (LFIA) with a novel label-free, non-optical sensing mechanism for protein binding detection was developed. The device consisted of bioconjugated microbeads as a test line and a three-dimensional (3D) electrode for sensing. By measuring the ionic conductivity shift across the bioconjugated beads at the surface of the 3D electrode, the protein binding could be detected. A model antigen, rabbit IgG, was used for quantitative evaluation with a limit of detection (LOD) of 50 nM for the LFIA. To improve the LOD, an electrokinetic preconcentration technique, faradaic ion concentration polarization (fICP), was implemented, resulting in an improvement in sensitivity and LOD.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Roshan Aggarwal, Bryn Ferris, Huiyan Li
Summary: A new microarray format, named compartmentalized linker array (CLA), was proposed, which consists of pre-prepared microarrays of chemical linkers in microliter compartments. CLA can bind and pattern bioreagents into microarrays by simply pipetting and incubating bioreagent solutions in compartments. The application of CLA was demonstrated for a multiplexed sandwich immunoassay measuring three cancer-related proteins.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Jiacheng He, Lang Zhou, Gangtong Huang, Jialiang Shen, Wu Chen, Chuanyu Wang, Albert Kim, Zhuoyu Zhang, Weiqiang Cheng, Siyuan Dai, Pengyu Chen, Feng Ding
Summary: This study presents a highly sensitive localized surface plasmon resonance imaging (LSPRi) immunoassay for label-free Interleukin 6 (IL-6) detection, achieving enhanced detection of serum IL-6, validated in vitro and in COVID-19 patients.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Oncology
Matthew T. Witkowski, Igor Dolgalev, Nikki A. Evensen, Chao Ma, Tiffany Chambers, Kathryn G. Roberts, Sheetal Sreeram, Yuling Dai, Anastasia N. Tikhonova, Audrey Lasry, Chunxu Qu, Deqing Pei, Cheng Cheng, Gabriel A. Robbins, Joanna Pierro, Shanmugapriya Selvaraj, Valeria Mezzano, Marla Daves, Philip J. Lupo, Michael E. Scheurer, Cynthia A. Loomis, Charles G. Mullighan, Weiqiang Chen, Karen R. Rabin, Aristotelis Tsirigos, William L. Carroll, Iannis Aifantis
Review
Chemistry, Multidisciplinary
Pengyu Chen, Feng Ding, Rong Cai, Ibrahim Javed, Wen Yang, Zhenzhen Zhang, Yuhuan Li, Thomas P. Davis, Pu Chun Ke, Chunying Chen
Review
Pharmacology & Pharmacy
Chao Ma, Yansong Peng, Hongtong Li, Weiqiang Chen
Summary: Organ-on-a-Chip technology has the potential to revolutionize drug development by mimicking the physiological environment and function of human organs on a chip. However, challenges remain in translating and commercializing this technology for pharmacological and medical applications.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2021)
Article
Chemistry, Physical
Zhuoyu Zhang, Lunan Liu, Chao Ma, Xin Cui, Raymond H. W. Lam, Weiqiang Chen
Summary: The PD-1 immune checkpoint-based therapy shows promise for treating GBM, but patient response varies. A computational model reveals differences in TAM-associated immunosuppression across GBM subtypes, suggesting a combination therapy targeting PD-1 and TAM-related pathways can enhance immune responses.
Article
Chemistry, Multidisciplinary
Chuanyu Wang, Chung-Hui Huang, Zhuangqiang Gao, Jialiang Shen, Jiacheng He, Alana MacLachlan, Chao Ma, Ya Chang, Wen Yang, Yuxin Cai, Yang Lou, Siyuan Dai, Weiqiang Chen, Feng Li, Pengyu Chen
Summary: Tumor-derived exosomes are crucial in cancer development, and quantitative analysis of exosomes can provide valuable predictive biomarkers. The role of exosomal PD-L1 in immune checkpoint therapy suggests the potential clinical application of exosomes.
Article
Chemistry, Multidisciplinary
Zhuangqiang Gao, Yujing Song, Te Yi Hsiao, Jiacheng He, Chuanyu Wang, Jialiang Shen, Alana MacLachlan, Siyuan Dai, Benjamin H. Singer, Katsuo Kurabayashi, Pengyu Chen
Summary: A machine-learning-assisted microfluidic nanoplasmonic digital immunoassay was developed for rapid and accurate monitoring of cytokine storm in COVID-19 patients, enabling simultaneous detection of multiple cytokines with wide working ranges and ultralow detection limits. The immunoassay demonstrated high throughput and efficiency, and the image processing method enhanced by CNN significantly reduced processing time while maintaining high statistical accuracy, showing promise as an intelligent immunoassay for next-generation immune monitoring.
Article
Nanoscience & Nanotechnology
Jiacheng He, Lang Zhou, Gangtong Huang, Jialiang Shen, Wu Chen, Chuanyu Wang, Albert Kim, Zhuoyu Zhang, Weiqiang Cheng, Siyuan Dai, Pengyu Chen, Feng Ding
Summary: This study presents a highly sensitive localized surface plasmon resonance imaging (LSPRi) immunoassay for label-free Interleukin 6 (IL-6) detection, achieving enhanced detection of serum IL-6, validated in vitro and in COVID-19 patients.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Zhuangqiang Gao, Chuanyu Wang, Jiacheng He, Pengyu Chen
Summary: This study reports an enhanced colorimetric ELISA method using Pd@Pt core@shell nanodendrites as peroxidase nanomimics, which can improve the detection sensitivity of cytokines to the femtomolar level. The method has a low detection limit, excellent reproducibility and specificity, and can be used for cytokine detection in human serum samples.
Article
Oncology
Lunan Liu, Chao Ma, Zhuoyu Zhang, Matthew T. Witkowski, Iannis Aifantis, Saba Ghassemi, Weiqiang Chen
Summary: The study established a computational model for CAR T-cell therapy, successfully predicting diverse responses of leukemia patients to treatment and identifying critical determinants related to patient responses. Combining peak and accumulated values of CAR T-cells or early-stage CAR T-cell dynamics can predict patient responses, which has significant implications for clinical treatment and management.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biophysics
Lang Zhou, Lunan Liu, Muammar Ali Chang, Chao Ma, Weiqiang Chen, Pengyu Chen
Summary: Real-time monitoring in the tumor microenvironment plays a crucial role in understanding cancer progression and treatment responses. However, challenges still exist in assessing limited tissue samples, establishing pre-clinical cancer models, monitoring static versus dynamic markers, and translating in vitro insights to clinical practice. This review focuses on state-of-the-art tumor-on-a-chip strategies and emerging platforms for real-time sensing and monitoring of the tumor microenvironment. The integration of sensors with tumor-on-a-chip platforms and associated challenges are also evaluated.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Pharmacology & Pharmacy
Xuejia Kang, Sanika Jadhav, Manjusha Annaji, Chung-Hui Huang, Rajesh Amin, Jianzhong Shen, Charles R. R. Ashby Jr, Amit K. K. Tiwari, R. Jayachandra Babu, Pengyu Chen
Summary: Disulfiram (DSF) is an approved drug for alcoholism treatment and has shown anticancer efficacy in preclinical studies. Supplementing DSF with copper (CuII) enhances its efficacy. However, clinical trials have not yielded promising results. Understanding the anticancer mechanisms of DSF/Cu (II) may lead to repurposing DSF as a new cancer treatment.
Article
Biochemistry & Molecular Biology
Xuejia Kang, Qi Wang, Siqi Wu, Chuanyu Wang, Manjusha Annaji, Chung-Hui Huang, Jianzhong Shen, Pengyu Chen, R. Jayachandra Babu
Summary: Therapeutic strategies for ARID1A-mutant ovarian cancers are limited. Higher basal ROS and lower basal GSH enhance the aggressive proliferation ability and metastatic property of OCCCs through EMT induction and immunosuppressive microenvironment. However, the aberrant redox homeostasis also increases the sensitivity of DQ-Lipo/Cu in mutant cells. DQ generates DDC in response to ROS, which together with Cu generates ROS cascade and targets GSH vulnerability, disrupting redox homeostasis and causing cancer cell death. The formed Cu(DDC)2 acts as a potent cytotoxic anti-cancer drug inducing immunogenic cell death, providing a promising strategy for managing cancer metastasis and drug resistance.
Article
Chemistry, Multidisciplinary
Rui Li, Walida Ali, Chao Ma, Apratim Bajpai, Ngoc Luu, Aarushi Varshney, Camden Riley Rowe, Weiqiang Chen
Summary: The strategic functionalization of the Wnt5a mimetic peptide ligand Foxy5 on a polydimethylsiloxane elastomer substrate enhances mechanotransduction and activation of cytotoxic CD8(+) T cells by triggering noncanonical Wnt signaling. This innovative mechanoregulatory ligand platform has broad applications in fundamental mechano-immunology research and the development of novel immunotherapies.
CHEMICAL COMMUNICATIONS
(2021)
Review
Nanoscience & Nanotechnology
Chuanyu Wang, Yuxin Cai, Alana MacLachlan, Pengyu Chen
IEEE NANOTECHNOLOGY MAGAZINE
(2020)