Article
Biotechnology & Applied Microbiology
Dragan Miscevic, Ju-Yi Mao, Teshager Kefale, Daryoush Abedi, Murray Moo-Young, C. Perry Chou
Summary: This study describes the development of a microbial bioprocess usingEscherichia colito produce high levels of 5-aminolevulinic acid (5-ALA). Through the implementation of the Shemin pathway and CRISPRi interference, metabolic engineering strategies were applied to direct carbon flux and enhance the biosynthesis of 5-ALA, achieving significant production yields.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Yuya Otaka, Kazutaka Kanai, Arisa Mori, Daiki Okada, Noriaki Nagai, Yohei Yamashita, Yoichiro Ichikawa, Kazuki Tajima
Summary: In this study, it was found that 5-ALA/SFC, administered to rats with endotoxin-induced uveitis, was able to suppress ocular inflammation by inhibiting NF-kappa B and activating the HO-1/Nrf2 pathways, showing similar effects to high-dose 5-ALA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Zhengshan Luo, Fei Pan, Yifan Zhu, Shanshan Du, Yifan Yan, Rui Wang, Sha Li, Hong Xu
Summary: Engineered synthetic scaffolds and system pathway engineering are crucial for enhancing metabolic fluxes in microbial production. In this study, the authors screened and optimized key enzymes, constructed a multienzyme complex assembly system, fine-tuned competitive metabolic pathways, and overcame obstacles to achieve efficient 5-aminolevulinic acid (5-ALA) synthesis. These strategies resulted in a significant increase in 5-ALA production.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhiyong Cui, Ziwei Zhu, Jinhong Zhang, Zhennan Jiang, Yinghang Liu, Qian Wang, Jin Hou, Qingsheng Qi
Summary: 5-ALA is a key precursor in the biosynthesis of various important compounds. In this study, engineered strains of Yarrowia lipolytica were used to produce 5-ALA efficiently. By optimizing culture conditions, the titer of 5-ALA reached 2216.8 mg/L in fed-batch fermentation, the highest achieved using yeast globally. This study demonstrates the potential of using SDH-deficient Y. lipolytica as a platform for efficient 5-ALA production.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Huaqing Chen, Biswajit Bhowmick, Yu Tang, Jesus Lozano-Fernandez, Qian Han
Summary: This study investigates the evolution of the mosquito enzyme HKT and its relationship with AGT in mosquitoes. The study identifies amino acid sites responsible for biochemical and functional evolution of HKT and builds a homology model to understand ligand-binding mechanism. The findings provide insights into the detoxification function of HKT and its potential applications in mosquito control.
Article
Biotechnology & Applied Microbiology
Anna Coenen, Manuel Ferrer, Karl-Erich Jaeger, Ulrich Schoerken
Summary: In this study, a novel enzyme cascade was developed for the synthesis of 12-aminododeceneoic acid, a precursor for nylon-12, starting from linoleic acid. The highest specific activities were found in the omega-transaminase from Aquitalea denitrificans. A one-pot enzyme cascade with TRAD and HPLCP-N achieved 59% conversions, while a 3-enzyme cascade with LOX-1, HPLCP-N, and TRAD reached up to 12% conversion to 12-aminododeceneoic acid from linoleic acid.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Meiru Jiang, Kunqiang Hong, Yufeng Mao, Hongwu Ma, Tao Chen, Zhiwen Wang
Summary: This article summarizes the biosynthetic pathways, regulatory mechanisms, and applications of 5-aminolevulinic acid (5-ALA) in the field of biotechnology. It highlights the progress in synthesizing 5-ALA via the C4/C5 pathway in microbial cells and discusses strategies for improving its biosynthesis and promoting its industrial application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Kenjiro Kaji, Tomohiro Yonezawa, Yasuyuki Momoi, Shingo Maeda
Summary: The study evaluated the efficacy of 5-aminolevulinic acid (5-ALA) in detecting canine urothelial carcinoma and its diagnostic accuracy. The results showed that 5-ALA can be used to detect urothelial carcinoma cells in dogs with relatively high diagnostic accuracy, and the fluorescence intensity correlated with the clinical condition of the cases.
Article
Biochemistry & Molecular Biology
Morena Petrini, Silvia Di Lodovico, Giovanna Iezzi, Luigina Cellini, Domenico Tripodi, Adriano Piattelli, Simonetta D'Ercole
Summary: The use of a new gel containing aminolevulinic acid and red light (ALAD-PDI) was tested to counteract bacterial biofilm growth on different titanium implant surfaces. The study evaluated the antibacterial efficacy of ALAD-PDI against biofilm growth on several titanium surfaces. Results showed that ALAD-PDI significantly reduced bacterial colony-forming units (CFUs) and biofilm biomass on titanium surfaces, and effectively killed bacteria.
Article
Biochemistry & Molecular Biology
Sara Sansaloni-Pastor, Emmanuel Varesio, Norbert Lange
Summary: 5-ALA-mediated photodynamic therapy (PDT) utilizes the heme biosynthesis pathway to selectively accumulate PpIX in cancer cells. However, the mechanism behind PpIX accumulation following PDT is still unknown. In this study, the authors evaluated the proteome changes in the heme pathway induced by 5-ALA supplementation. They found that porphyrins play a crucial role in heme biosynthesis modulation, while 5-ALA alone does not cause significant changes in the pathway. Understanding the regulation of heme enzymes could improve the design of PDT treatments.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Forestry
Zhiyin Jiao, Shuo Han, Xiao Yu, Mengbo Huang, Conglong Lian, Chao Liu, Weilun Yin, Xinli Xia
Summary: The study suggests that ALA pretreatment significantly improved the stress tolerance of poplar, decreasing membrane lipid peroxidation and promoting photosynthesis and antioxidant capacity of leaves. Additionally, the results indicate that ALA may increase poplar's salt tolerance by mediating the activity of Na+ transporters and aquaporins.
Review
Biochemistry & Molecular Biology
Pamela Pignatelli, Samia Umme, Domenica Lucia D'Antonio, Adriano Piattelli, Maria Cristina Curia
Summary: Cancer is a major global cause of death, and photodynamic therapy utilizing reactive oxygen species has shown promise as an anticancer treatment. Specifically, 5-aminolevulinic acid-photodynamic therapy (5-ALA-PDT) has been effective in treating various types of cancer. This therapy uses a photosensitizer activated by light to produce ROS, leading to apoptotic activity in malignant tissues. This review highlights the efficacy of 5-ALA-PDT in treating different cancer pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Polymer Science
Simonetta D'Ercole, Teocrito Carlesi, Tatiane Cristina Dotta, Tania Vanessa Pierfelice, Emira D'Amico, Domenico Tripodi, Giovanna Iezzi, Adriano Piattelli, Morena Petrini
Summary: The aim of this study was to discuss the use of 5-aminolevulinic acid with red LED light in endodontic treatment. It is reported that the persistence of microorganisms is the main cause of endodontic treatment failure. Photodynamic therapy has become a promising antimicrobial strategy for endodontic treatment, as it is easy to apply, quick, and does not allow microbial resistance. Recent studies have shown that the combination of 5-aminolevulinic acid and red LED light has good results against various types of bacteria, including Enterococuss faecalis. A case report showed a significant decrease in bacterial count after irrigation with a gel containing 5% of 5-aminolevulinic acid and subsequent irradiation with red LED light. The use of 5-aminolevulinic acid with red LED light is therefore concluded to be effective in endodontic treatment.
Review
Biochemistry & Molecular Biology
Yoshinori Harada, Yasutoshi Murayama, Tetsuro Takamatsu, Eigo Otsuji, Hideo Tanaka
Summary: 5-Aminolevulinic acid (5-ALA) is a natural amino acid that can accumulate in cancer cells and be used for tumor visualization. However, its diagnostic applicability is challenging due to interference from tissue autofluorescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Patricia Regina Menezes, Carlos Eduardo Madureira Trufen, Flavio Lichtenstein, Diogo Vieira da Silva Pellegrina, Eduardo Moraes Reis, Janice Onuki
Summary: 5-aminolevulinic acid (5-ALA) is the first precursor of the heme biosynthesis pathway and is accumulated in acute intermittent porphyria (AIP), which is characterized by porphobilinogen deaminase deficiency. A long-term manifestation in symptomatic AIP patients is an increased incidence of hepatocellular carcinoma (HCC). High concentrations of 5-ALA can induce deleterious pro-oxidant effects, leading to apoptosis, DNA damage, mitochondrial dysfunction, and altered expression of proteins related to carcinogenesis. This study investigated the transcriptional changes and molecular pathways in HepG2 cells exposed to 5-ALA, providing valuable insights into the toxicity of 5-ALA and the pathophysiology of AIP and HCC. The findings can contribute to the development of better therapies and biomarkers for AIP/AHP and associated HCC.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biotechnology & Applied Microbiology
Johannes Moeller, Katrin Korte, Ralf Poertner, An-Ping Zeng, Uwe Jandt
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Review
Biotechnology & Applied Microbiology
An-Ping Zeng
BIOTECHNOLOGY ADVANCES
(2019)
Review
Biotechnology & Applied Microbiology
Franziska Enzmann, Markus Stoeckl, An-Ping Zeng, Dirk Holtmann
ENGINEERING IN LIFE SCIENCES
(2019)
Article
Biotechnology & Applied Microbiology
Rebekka Schmitz, Wael Sabra, Philipp Arbter, Yaeseong Hong, Tyll Utesch, An-Ping Zeng
ENGINEERING IN LIFE SCIENCES
(2019)
Article
Chemistry, Medicinal
Philipp Nicolas Depta, Uwe Jandt, Maksym Dosta, An-Ping Zeng, Stefan Heinrich
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2019)
Article
Biotechnology & Applied Microbiology
Tyll Utesch, Wael Sabra, Christin Prescher, Julian Baur, Philipp Arbter, An-Ping Zeng
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Editorial Material
Biotechnology & Applied Microbiology
An-Ping Zeng, Ralf Takors, Thomas Bley
ENGINEERING IN LIFE SCIENCES
(2019)
Article
Biochemical Research Methods
Yujun Zhang, Chengwei Ma, Wanda Dischert, Philippe Soucaille, An-Ping Zeng
BIOTECHNOLOGY JOURNAL
(2019)
Article
Biochemical Research Methods
Matthias Wurm, Sibel Ilhan, Uwe Jandt, An-Ping Zeng
ANALYTICAL BIOCHEMISTRY
(2019)
Article
Biochemical Research Methods
Xinyi Zhang, Mei Li, Yingying Xu, Jie Ren, An-Ping Zeng
JOURNAL OF BIOLOGICAL ENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Johannes Moeller, Krathika Bhat, Kristoffer Riecken, Ralf Poertner, An-Ping Zeng, We Jandt
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Biochemical Research Methods
Chuang Wang, Jie Ren, Libang Zhou, Zhidong Li, Lin Chen, An-Ping Zeng
ACS SYNTHETIC BIOLOGY
(2019)
Article
Biochemical Research Methods
Libang Zhou, Jie Ren, Zhidong Li, Jinglei Nie, Chuang Wang, An-Ping Zeng
ACS SYNTHETIC BIOLOGY
(2019)
Article
Agricultural Engineering
Philipp Arbter, Aakanksha Sinha, Julie Troesch, Tyll Utesch, An-Ping Zeng
BIORESOURCE TECHNOLOGY
(2019)
Review
Biochemical Research Methods
Yaeseong Hong, Jie Ren, Xinyi Zhang, Wei Wang, An-Ping Zeng
CURRENT OPINION IN BIOTECHNOLOGY
(2020)